Method for constructing DNA library and application of method

Abstract

The invention discloses a method for constructing a DNA library and application of the method. The method for constructing the DNA library comprises the steps: providing DNA of a chimeric marker, wherein the DNA of the chimeric marker has three-dimensional structure information; carrying out transposition treatment on the DNA of the chimeric marker to obtain a transposition product; carrying out capture treatment on the transposition product to obtain captured DNA; and carrying out amplification treatment on the captured DNA so as to obtain the DNA library. The library construction method is simple in steps, short in consumed time, particularly suitable for library construction of trace DNA samples, high in effective data proportion of sequencing and low in noise single-end suspension value.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular, to a method for constructing a DNA library and its application, more specifically, to a method for constructing a DNA library, a method for obtaining chromatin interaction information in individual cells, and a method for obtaining A method for information, a method for three-dimensional genome research, a method for prenatal diagnosis or cancer screening, a kit, and the use of the kit in three-dimensional genome assembly library or prenatal diagnosis or cancer screening. Background technique [0002] There are many steps in the existing next-generation sequencing library construction technology, especially the multi-step operations from the final end repair to PCR are likely to cause the loss of effective fragments. It is more significant in the three-dimensional genomic Hi-C library construction. The biotin-labeled chimeric DNA is relatively small as a library construction te...

AI Technical Summary

Problems solved by technology

[0002] There are many steps in the existing next-generation sequencing library construction technology, especially the multi-step operations from the final end repair to PCR are likely to cause the loss of effective fragments

It is more significant in the three-dimensional genome Hi-C library construction. The biotin-labeled chimeric DNA is relatively small as a library construction template, so the loss of effective fragments after fishing will directly affect the quality of the final library.

In addition, the fragment screening step is set to make the library adapt to the sequencing-by-synthesis principle of illumina, but too long fragments will lead to poor quality of sequencing data

At the same time, a considerable part of the available library fragments will be screened out due to fragment length issues, especially under the conditions of nanogram-level templates, screening will lead to a reduction in the number of effective libraries, directly affecting the effective data ratio

[0003] Therefore, existing library construction methods need to be improved

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