Streptococcus thermophilus and enrichment culture method and application thereof

A technology of streptococcus thermophilus and proliferation medium, applied in the field of streptococcus thermophilus DMST-H2 and its proliferation and cultivation, can solve the problems of insufficient optimization conditions, achieve potential probiotic value, low cost, and increase the number of viable bacteria

Active Publication Date: 2019-12-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above technologies pointed out the optimization direction of proliferation culture, mainly including the optimization of medium components and the optimization of culture conditions such as temperature, pH, inoculum size, etc., but there are still problems such as insufficient optimization conditions

Method used

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  • Streptococcus thermophilus and enrichment culture method and application thereof
  • Streptococcus thermophilus and enrichment culture method and application thereof
  • Streptococcus thermophilus and enrichment culture method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 A strain of Streptococcus thermophilus DMST-H2 derived from traditional fermented dairy products in China

[0035] This example is a strain of Streptococcus thermophilus DMST-H2 derived from traditional Chinese fermented milk, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC for short) with the preservation number GDMCC 60642. The biological characteristics of the Streptococcus thermophilus (Streptococcus thermophilus) strain DMST-H2 are: streaked and separated on the MRS medium plate, cultivated at 37°C for 48 hours, the colonies are round, raised, with neat edges, milky white colonies, and the surface is wet and smooth . Gram-stained microscopic examination shows that it is a Gram-positive bacterium, spherical in shape, paired in pairs, with long chains and no spores. Physiological and biochemical test results showed that the strain KOH test was negative, catalase test was negative, facultative anaerobic, able to hydrolyze starch, ...

Embodiment 2

[0036] The separation and purification of embodiment 2 Streptococcus thermophilus DMST-H2

[0037] This example is a method for the isolation and purification of Streptococcus thermophilus DMST-H2 derived from traditional Chinese fermented milk. The method is carried out as follows:

[0038] (1) Shake the home-made yogurt sample in Inner Mongolia with a vortex shaker for 5 minutes. Under sterile conditions, draw 0.5mL milk sample and mix it in 4.5mL sterile saline. This is 10 -1 Diluent, so, diluted to 10 -7 , select 10 -5 、10 -6 、10 -7 For dilution, draw 100 μL and spread it on the MRS plate, and place it in an anaerobic culture tank for 48 hours at 37°C;

[0039] (2) Observe the growth of the colony, and select the characteristic single colony after the characteristic bacteria grow on the plate;

[0040] (3) Streak inoculate a single colony onto the MRS solid medium, incubate at 37°C for 24 hours, pick a single colony and streak again, and perform 6 purifications in thi...

Embodiment 3

[0044] The optimization of embodiment 3 Streptococcus thermophilus DMST-H2 substratum

[0045] (1) Single factor test and results

[0046] In the carbon source single factor test, based on the MRS medium, two parts of mannose, lactose, maltose, sucrose, and soluble starch were used to replace the carbon source (glucose) in the MRS medium, and the medium without carbon source was set as Blank control, MRS medium as negative control. Inoculate Streptococcus thermophilus DMST-H2 in logarithmic growth phase, culture at 37°C for 24 hours, measure OD 600 , choose the best carbon source according to the pros and cons of cell growth. The results are shown in Figure 2(a), the cell density obtained by culturing Streptococcus thermophilus DMST-H2 with lactose was the highest, followed by sucrose and mannose. The cell density of the lactose test group was significantly higher than that of other sugars (p<0.05). Therefore, lactose was selected for further research.

[0047] In the nit...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses streptococcus thermophilus and an enrichment culture method and application thereof. A strain DMST-H2 is separated from homemade yogurt of the Inner Mongolia family, and is preserved in the Guangdong microbial culture collection center (GDMCC) at April 16, 2019, and the preservation number is GDMCC 60642. By optimizing an enrichment culture medium, the viable count of the strain can be increased to 10<9> CFU/mL or above. The viable count under optimal conditions can reach 4.2*10<9> CFU/mL, and is 37.8 times that before the conditions are optimized. The strain is more suitable for intestines of Chinese, and lower in cost. In addition, the strain has the capacity of resisting acid, cholate and artificial gastrointestinal fluid, and meanwhile has the outstanding antioxidant capacity, especially DPPH removal capacity.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Streptococcus thermophilus DMST-H2 derived from traditional Chinese fermented milk and a method for multiplying and cultivating the same. Background technique [0002] Streptococcus thermophilus is a starter strain of various fermented products, and it is the second most important industrial lactic acid bacteria species after Lactococcus lactis. Humans consume about 10 21 Streptococcus thermophilus live bacteria, resulting in a market value of this strain reached 40 billion US dollars. In the dairy industry, Streptococcus thermophilus is usually used together with Lactobacillus delbrueckii subsp. bulgaricus as the main fermentation strain of fermented milk direct starter starter. During the fermentation process, Streptococcus thermophilus can quickly acidify the medium, inhibiting the growth of bacteria, yeast and other bacteria. Most Streptococci thermophilus p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12C12R1/46
CPCC12N1/20A23C9/12C12R2001/46C12N1/205
Inventor 刘冬梅胡金双黄泳尧邝嘉华
Owner SOUTH CHINA UNIV OF TECH
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