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Potassium ion detection kit

A detection kit and potassium ion technology are applied in the field of serum potassium ion detection kits, which can solve problems such as poor stability and inaccurate detection results, and achieve the effect of improving sensitivity

Inactive Publication Date: 2019-12-20
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Evaluation: This method is easy to operate, has good linearity, repeatability, analytical sensitivity and anti-interference ability, and is suitable for automatic biochemical analyzers. However, the stability of the kits used to determine potassium ions on the market will become worse after long-term storage. Poor, eventually leading to inaccurate detection results

Method used

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Embodiment 1

[0049] Potassium ion detection kit of the present invention is made up of reagent R1 and reagent R2, wherein:

[0050] The composition and content of reagent R1 are as follows:

[0051]

[0052] The composition and content of reagent R2 are as follows:

[0053]

[0054]

[0055] Calibrator and quality control substance used in the kit test of embodiment 1 are respectively:

[0056] Calibrator: The serum potassium content of RANDOX926 is 4.22mmol / L.

[0057] Quality control product: the target value of RANDOX1005 serum potassium is 4.22mmol / L, and the target value range: 3.88~4.56mmol / L.

Embodiment 2

[0059]

[0060] The composition and content of the reagent R2 are as follows:

[0061]

[0062] Calibrators and quality control substances used in the kit test of embodiment 2 are respectively:

[0063] Calibrator: The serum potassium content of RANDOX926 is 4.22mmol / L.

[0064] Quality control product: the target value of RANDOX1005 serum potassium is 4.22mmol / L, and the target value range: 3.88~4.56mmol / L.

[0065] When the kit of above-mentioned embodiment 1 and embodiment 2 is in use, its assay method is to adopt the Hitachi 7180 full-automatic analyzer etc. with double reagent function, utilize rate method to measure, and operate as follows:

[0066] Add 5 μl of purified water, sample or calibrator, then add 180 μl of R1 reagent for pre-incubation for 5 minutes, then add 60 μl of reagent R2, start recording the absorbance value A1 after 30 seconds, and read the absorbance A2 after 5 minutes of reaction, and calculate ΔA / min .

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Abstract

The invention discloses a potassium ion detection kit and relates to the technical field of biology. The potassium ion detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 isprepared from the following components of a buffer solution, a cryptand, phosphoenolpyruvate, adenosine diphosphate, alpha-ketoglutaric acid, reductive coenzyme I, magnesium chloride, propylene glycol-monomethyl ether and 4-methyl phenylboric acid; and the reagent R2 is prepared from the following components of the buffer solution, glutamate dehydrogenase, pyruvate kinase, lactic dehydrogenase, albumin bovine serum, calcium chloride and the propylene glycol-monomethyl ether. According to the potassium ion detection kit, the stability of the potassium ion detection kit can be improved on the premise of not affecting the accuracy and the analysis sensitivity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a serum potassium ion detection kit. Background technique [0002] Potassium in the human body mainly comes from food, more than 90% of the potassium in the food is absorbed in the intestinal tract within a short period of time, and 90% of the potassium absorbed into the blood is excreted from the kidney within 4 hours. Most of the potassium ions (98%) exist in the cells, and a small amount exists in the extracellular fluid, and the concentration is constant. Average K content in tissue cells + 150mmol / L, red blood cells contain K + About 105mmol / L, containing K in serum + About 4~5mmol / L. Potassium ions in the body are constantly exchanged between cells and body fluids to maintain a dynamic balance. Potassium is the main cation to maintain the physiological activities of cells, and plays an important role in maintaining normal osmotic pressure and acid-base balance of the body,...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/31
CPCG01N21/278G01N21/31G01N33/54393
Inventor 史建国王珍李倩甘宜梧李静谢清华胡晓飞
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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