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Anti-tumor composition and pharmaceutical application thereof

A composition and anti-tumor technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as being unsuitable for surgery, radiotherapy, chemotherapy, preparation or high cost, and large toxic and side effects, and achieve rapid drug efficacy. , Easy to take, high content of active ingredients

Active Publication Date: 2019-12-20
QINGDAO WOMEN & CHILDREN HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. Those who are not suitable for surgery, radiotherapy, chemotherapy and those who are unwilling to be treated by western medicine. 3. Those who are ineffective in advanced cancer pain by western medicine
The most effective drug classes for targeted therapy of anaplastic thyroid carcinoma (ATC) are paclitaxel (paclitaxel or docetaxel), benzophenones, and platinum (cisplatin or carboplatin), but there are some sources that are not easy to prepare or cost less. High or toxic side effects and other issues

Method used

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  • Anti-tumor composition and pharmaceutical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Codonopsis polysaccharide extraction:

[0040] Dried Codonopsis pilosula was purchased from Shuyu Mining Pharmacy in Jinan, Shandong, and was identified as the dried root of Cdoonopisis pilosula (Famch.) Nannf, a plant of the Campanulaceae family, by the Department of Pharmacognosy, School of Pharmacy, Shandong University.

[0041] The preparation method of Codonopsis polysaccharide is as follows: take a certain amount of Codonopsis medicinal materials, remove impurities and mildew, rinse with clean water, and dry; chop, cut Codonopsis into 3-5cm sections, put them in a cooking tank for decoction, add 6 times the volume of cyclohexane, heated to reflux for 5 hours, discarded the extract, then added 6 times the volume of cyclohexane, heated and refluxed for 5 hours, discarded the extract, evaporated the remaining filter residue to dry the solvent; took 500g of filter residue, and used distilled water as the extraction solvent , under the condition that the extraction tem...

Embodiment 2

[0047] Codonopsis polysaccharides were prepared as described in Examples, and azadirachtin was purchased from SigmaAldrich.

[0048] Codonopsis polysaccharides and azadirachtin were prepared into 100 μM stock solutions with dimethyl sulfoxide (DMSO) respectively, aliquoted in small quantities, stored at -20°C for later use, and diluted to the required final concentration with corresponding cell culture medium before use . Prepare the final concentration as shown in the table below (μM):

[0049] Codonopsis polysaccharide and azadirachtin composition solution prepared in the laboratory of table 1

[0050]

[0051] 1. Cell culture: PTC-1 cells were cultured according to conventional methods in the art.

[0052] 2. Select PTC-1 cells in the logarithmic growth phase, digest with trypsin, make cell suspension with RPMI1640 medium containing calf serum, inoculate in a culture plate, inoculate 1 mL per well, 37 ° C, 5% CO 2 Cultivate in the incubator for 24h.

[0053] 3. The ...

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Abstract

The invention provides an anti-tumor composition with synergistic effect and pharmaceutical application thereof. The anti-tumor composition comprises a radix codonopsis extract and an azadirachta indica extract. The radix codonopsis extract is selected from radix codonopsis polysaccharide, the azadirachta indica extract is selected from azadirachtin, and the molar ratio of the radix codonopsis extract to the azadirachta indica extract is 10:(3-20). Experimental results show that the components of the traditional Chinese medicine composition provided by the invention have synergistic function and can effectively inhibit the proliferation of PTC-1 cells and induce the apoptosis of tumor cells. Meanwhile, the traditional Chinese medicine composition has wide raw material sources, can be conveniently prepared into various dosage forms together with a pharmaceutical carrier, and is convenient to take clinically. The prepared radix codonopsis extract has the advantages of high content of active ingredients, less impurities, quick absorption after being compounded with the azadirachtin, capability of rapidly exerting drug effect and low clinical dosage, and is stable in process and suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition with antithyroid cancer activity and its pharmaceutical application. Background technique [0002] Tumor is a new organism formed by the body under the action of various carcinogenic factors, a certain cell in the local tissue loses its normal regulation of its growth at the gene level, resulting in abnormal clonal proliferation. It is generally believed that tumor cells are monoclonal, that is, all cells in a tumor are descendants of a mutant cell. Tumors are generally divided into benign and malignant. All malignant tumors are collectively called cancer. Tumor cells have abnormal morphology, metabolism and function, and have lost the ability to differentiate and mature to varying degrees. Tumors grow vigorously and are relatively autonomous. Even if the tumorigenic factors no longer exist, they can still grow continuousl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K31/343A61P35/00A61K36/58A61K36/344
CPCA61K31/715A61K31/343A61P35/00A61K36/344A61K36/58A61K2300/00Y02A50/30
Inventor 陈旭伟房辉
Owner QINGDAO WOMEN & CHILDREN HOSPITAL
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