He staining process of automatic staining machine

A dyeing machine and automatic technology, applied in the field of automatic dyeing machine He dyeing process, can solve the problems of poor dyeing stability, uneven staining of cell nucleus, affecting the judgment of test operators, etc., to increase the dyeing strength and dyeing stability. , The effect of high test accuracy and clear dyeing boundary

Inactive Publication Date: 2019-12-10
宋建华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing He staining process, the cells and tissues are stained with only one tank of hematoxylin staining solution and one tank of eosin solution, and there is no complementary color measure for the undyed color, and the dyeing strength is not enough
[0004] In addition, the existing method has the problems of different shades of nuclear staining and cytoplasmic staining, too dark or too shallow staining, poor staining stability, insufficiently bright staining of the nucleus and cytoplasm, and insufficient contrast, which affects test operators. judgment, thus affecting the accuracy of the final experimental judgment

Method used

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  • He staining process of automatic staining machine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Automatic dyeing machine and dyeing process, including the following steps:

[0023] (1) Dewaxing: dewax the tissue sections in xylene for four times, each dewaxing time is 5 min;

[0024] (2) Elution of xylene: after dewaxing, put the slice into absolute ethanol to elute xylene three times, each time for 1 min;

[0025] (3) Tissues into water: After elution of xylene, use high-concentration to low-concentration alcohols to carry out the tissue watering process sequentially, soak in each concentration of alcohol for 1 min, and then wash with water. High-concentration to low-concentration alcohol refers to the volume Concentrations of 95%, 85%, and 75% gradient alcohol;

[0026] (4) Cell nucleus staining: After washing with water, put the slices into two tanks of hematoxylin staining solution for staining, soak for 5 minutes in each tank, put the slices into the newer tank of hematoxylin staining solution first, and then put them into the older tank. Hematoxylin staini...

Embodiment 2

[0033] Automatic dyeing machine and dyeing process, including the following steps:

[0034] (1) Dewaxing: Dewax the tissue sections in xylene for four times, each dewaxing time is 6 min;

[0035] (2) Elution of xylene: after dewaxing, put the slice into absolute ethanol to elute xylene three times, each time for 2 minutes;

[0036] (3) Tissues into water: After elution of xylene, use high-concentration to low-concentration alcohols to carry out the tissue watering process sequentially, soak in each concentration of alcohol for 2 minutes, and then wash with water. High-concentration to low-concentration alcohol refers to the volume Concentrations of 95%, 85%, and 75% gradient alcohol;

[0037] (4) Cell nucleus staining: after washing, put the slices into two tanks of hematoxylin staining solution for staining, soak for 7 minutes in each tank, put the slices into the newer tank of hematoxylin staining solution first, and then put them into the older tank. Hematoxylin staining ...

Embodiment 3

[0044] Automatic dyeing machine and dyeing process, including the following steps:

[0045] (1) Dewaxing: Dewax the tissue sections in xylene for four times, each dewaxing time is 8 min;

[0046] (2) Elution of xylene: after dewaxing, put the slice into absolute ethanol to elute xylene three times, each time for 3 minutes;

[0047] (3) Tissues into water: After elution of xylene, use high-concentration to low-concentration alcohols to carry out the tissue watering process sequentially, soak in each concentration of alcohol for 3 minutes, and then wash with water. High-concentration to low-concentration alcohol refers to the volume Concentrations of 95%, 85%, and 75% gradient alcohol;

[0048] (4) Cell nucleus staining: After washing with water, put the slices into two tanks of hematoxylin staining solution for staining, soak for 8 minutes in each tank, put the slices into the newer tank of hematoxylin staining solution first, and then put them into the older tank. The hemato...

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Abstract

The invention discloses a He staining process of an automatic staining machine. The He staining process comprises the following steps: (1) dewaxing; (2) eluting xylene; (3) putting tissue into water;(4) staining cell nucleuses; (5) performing cytoplasm staining; (6) cleaning with alcohol; (7) achieving tissue transparency; (8) sealing and fixing. When the process provided by the invention is usedfor staining slice tissues, the cell nucleuses and cytoplasm are bright in coloring, uniform in coloring, good in coloring stability and clear in color contrast of the cell nucleuses and the cytoplasm, so that the judgment of test results is more accurate.

Description

technical field [0001] The invention relates to the technical field of biological tissue sample processing, in particular to an automatic dyeing machine and a dyeing process. Background technique [0002] Hematoxylin-eosin staining, referred to as HE staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm stain purple blue; eosin is an acid dye, which mainly makes the components in the cytoplasm and extracellular matrix red. HE staining is the most basic and widely used technical method in the teaching and scientific research of histology, embryology and pathology. [0003] In the existing He staining process, the cells and tissues are stained with only one tank of hematoxylin staining solution and one tank of eosin solution, and there is no complementary color measure for those that are not dyed, and the dyeing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 宋建华杨达平李雄刘勇青
Owner 宋建华
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