Preparation and application method of biocontrol bacteria of mikania micrantha

Abstract

The invention discloses a preparation and application method of biocontrol bacteria of mikania micrantha. The preparation and application method comprises the steps: pure culture and acquisition of to-be-selected pathogenic bacteria; safety test of the to-be-selected pathogenic bacteria; toxicity test of a bactericide for the biocontrol bacteria; temperature test of the biocontrol bacteria; pathogenicity retention of the biocontrol bacteria; propagation expansion of the biocontrol bacteria; and preparation of a biocontrol bacterium preparation. The equipment and process requirement are simple,the obtained biocontrol bacteria of mikania micrantha are capable of effectively infecting mikania micrantha and realizing continuous control, stable in field performance, safe to non-target crops and environment-friendly. The prevention and control of the mikania micrantha are realized by taking target pathogenic bacteria screening and pathogenicity retention as cores; and due to the safety evaluation of the non-target crops and effective control on the pathogenic bacteria, the protection for the non-target crops is realized, the spread of the mikania micrantha is better delayed, the hazardsof the mikania micrantha is relieved, and the biocontrol bacteria are suitable for preventing and controlling the mikania micrantha in a forest.

Description

technical field [0001] The invention relates to a preparation and application method of a mikania micrantha biocontrol fungus, which belongs to the field of plant protection. Background technique [0002] Mikania ( Mikania micrantha Kunth) is a vine of Compositae, which covers the host plant by climbing and forming a thick blanket-like layer of branches and leaves, so that the host plant cannot perform normal photosynthesis and dies; the reproductive ability is extremely strong, and once it occurs, it can quickly become a disaster. [0003] Mikania micrantha was included in the first list of invasive alien species in China in 2002. It was first discovered in Maoming in 2009, and has spread wildly since then. By the end of 2015, the area of ​​occurrence was 31,000 mu, and the area of ​​damage was about 18,000 mu, which had a huge impact on local native species. . At present, Mikania micrantha is mainly treated by manual eradication and chemical herbicides. The cost is high,...

AI Technical Summary

Problems solved by technology

[0005] Up to now, there are many domestic reports on the utilization of Puccinia micrantha micrantha, and good results have been achieved, but it is still in the experimental stage, and the risk of invasion of alien species is still difficult to eliminate; although the introduced Anna Jane butterfly has good effects in the breeding environment However, during stocking, it was found that it could not normally settle down and form a community

The reports on the use of local native species to carry out biological control attempts mainly focus on the use of dodder, and there are also reports on the impact of Mikania micrantha and Mikania micrantha on Mikania micrantha, but they are all in the laboratory stage and have not been widely applied