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Metal framework compound nanocarrier delivery system for targeted activation of cd44 molecule, preparation method and use thereof

A technology of nano-carrier and delivery system, which can be applied to inactive components of polymer compounds, preparations for in vivo tests, nano-drugs, etc., and can solve problems such as non-existence

Active Publication Date: 2021-02-26
BEIJING INNO MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the following problems will be encountered in the research and development of targeted drug delivery systems targeting CD44: if the affinity between CD44 and HA on the surface of target cells is not enough to provide significant specificity, then such targeted drug delivery systems will be difficult to obtain. There will be no specific targeting properties
[0014] So far, there is no report on the expression status of CD44 on the surface of macrophages, monocytes, endothelial cells, lymphocytes and smooth muscle cells mainly present in vulnerable plaques and its affinity for HA, nor does it exist. Any targeted drug delivery capable of stable and sustained release of drugs designed for the diagnosis or treatment of vulnerable plaques or diseases associated with vulnerable plaques using the interaction of HA and CD44 and the specific microenvironment of vulnerable plaques system prior art

Method used

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  • Metal framework compound nanocarrier delivery system for targeted activation of cd44 molecule, preparation method and use thereof
  • Metal framework compound nanocarrier delivery system for targeted activation of cd44 molecule, preparation method and use thereof
  • Metal framework compound nanocarrier delivery system for targeted activation of cd44 molecule, preparation method and use thereof

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specific Embodiment approach

[0120] The present invention will be further illustrated by specific examples below, however, it should be understood that these examples are only used for more detailed description, and should not be construed as limiting the present invention in any form.

[0121] This section provides a general description of the materials and test methods used in the tests of the present invention. While many of the materials and methods of manipulation which are employed for the purposes of the invention are well known in the art, the invention has been described here in as much detail as possible. It will be apparent to those skilled in the art that, in the context and context, the materials used and methods of operation used in the present invention are known in the art unless otherwise specified.

Embodiment 1

[0122] Example 1 Preparation of loaded rosuvastatin (R) metal framework compound (MOF-(R)-HA) nano-delivery system coupled with hyaluronic acid (HA)

[0123] This patent uses terephthalic acid (H 2 BDC) and magnesium ions (Mg 2+ ) Prepare MOF-7 drug carrier, load therapeutic drugs and contrast agents, and connect hyaluronic acid targeting molecules on its surface to realize target recognition and drug delivery.

[0124] 1.1 Preparation of blank MOF-7 carrier: H 2 BDC (5g) was dissolved in 10mL DMF, and 10mg mL was added dropwise under stirring at 50°C -1 Mg(NO 3 ) 2Aqueous solution, a white emulsion can be seen, continue to react under these conditions for 24 hours, and centrifuge at high speed to obtain a white product of MOF-7. figure 1 It is the X-ray diffraction peak (XRD) of blank carrier MOF-7.

[0125] 1.2 Preparation of rosuvastatin (R) metal framework compound (MOF-(R)) nano-delivery system: 10mgmL -1 Mg(NO 3 ) 2 Aqueous solution and 10mg mL -1 Rosuvastatin...

Embodiment 2

[0127] Example 2 Preparation of rosuvastatin (R) MOF-7 nanocarrier (MOF-(R)-SP) delivery system coupled with selectin (SP)

[0128] MOF-(R) loaded with rosuvastatin was prepared according to the method in Example 1.

[0129] Dissolve 1 mg of selectin SP in PBS buffer solution, add 0.5 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) and 0.5 mg of N-hydroxy The sulfosuccinimide (sulfo-NHS) coupling reagent activates the carboxyl group. After stirring the reaction at room temperature for 1 hour. Configure it to 1mg mL -1 1mL solution was taken and dissolved in the purified MOF-(R) solution to realize the coupling of SP on MOF-(R) to obtain the targeted recognition nanocarrier MOF-(R)-SP. image 3 Infrared spectra of blank MOF-7 and MOF-(R)-SP metal framework nanocarriers.

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Abstract

A metal framework compound nano-carrier delivery system for targeting activated CD44 molecules, its preparation method and use, the surface of the nano-carrier is partially modified by a targeting ligand, and the targeting ligand is capable of interacting with the activated The CD44 molecule specifically binds to the ligand. The metal framework compound nanocarrier delivery system can be used for the diagnosis, prevention and treatment of vulnerable plaques or diseases related to vulnerable plaques.

Description

[0001] Cross References to Related Applications [0002] This application claims the priority of the Chinese invention patent application No. CN201810060265.9 submitted on January 22, 2018, which is incorporated herein by reference. technical field [0003] The invention belongs to the technical field of targeted drug delivery, and in particular relates to a nano-carrier for targeting and activating CD44 molecules, especially for targeting vulnerable plaques, in particular to a metal framework compound nano-delivery system. The present invention also relates to the preparation method and application of the nano-carrier, especially the metal framework compound nano-delivery system, especially the application in the diagnosis, prevention and treatment of vulnerable plaque or diseases related to vulnerable plaque. Background technique [0004] At present, acute cardiovascular events, mainly acute myocardial infarction and sudden cardiac death, have become the number one killer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/00A61K47/06A61K49/00A61P7/00A61P9/00
CPCA61P7/00A61P9/00A61K51/1234A61K49/1812A61K49/0409A61K47/6911A61K47/6913A61K9/1271A61K9/1272A61K9/0019A61K9/127A61K47/10A61K47/42A61K47/36A61P9/10A61K49/0002A61K49/0084A61K49/04A61K45/06B82Y5/00
Inventor 马茜孙洁芳
Owner BEIJING INNO MEDICINE CO LTD
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