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Mesoporous/hollow silica nanocarrier delivery system for targeted activation of CD44 molecules, its preparation method and use

A technology of nano-carrier and delivery system, which can be used in preparations for in vivo tests, non-active components of polymer compounds, nano-drugs, etc., and can solve problems such as non-existence

Active Publication Date: 2021-02-26
BEIJING INNO MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the following problems will be encountered in the research and development of targeted drug delivery systems targeting CD44: if the affinity between CD44 and HA on the surface of target cells is not enough to provide significant specificity, then such targeted drug delivery systems will be difficult to obtain. There will be no specific targeting properties
[0014] So far, there is no report on the expression status of CD44 on the surface of macrophages, monocytes, endothelial cells, lymphocytes and smooth muscle cells mainly present in vulnerable plaques and its affinity for HA, nor does it exist. Any targeted drug delivery capable of stable and sustained release of drugs designed for the diagnosis or treatment of vulnerable plaques or diseases associated with vulnerable plaques using the interaction of HA and CD44 and the specific microenvironment of vulnerable plaques system prior art

Method used

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  • Mesoporous/hollow silica nanocarrier delivery system for targeted activation of CD44 molecules, its preparation method and use
  • Mesoporous/hollow silica nanocarrier delivery system for targeted activation of CD44 molecules, its preparation method and use
  • Mesoporous/hollow silica nanocarrier delivery system for targeted activation of CD44 molecules, its preparation method and use

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specific Embodiment approach

[0122] The present invention will be further illustrated by specific examples below, however, it should be understood that these examples are only used for more detailed description, and should not be construed as limiting the present invention in any form.

[0123] This section provides a general description of the materials and test methods used in the tests of the present invention. While many of the materials and methods of manipulation which are employed for the purposes of the invention are well known in the art, the invention has been described here in as much detail as possible. It will be apparent to those skilled in the art that, in the context and context, the materials used and methods of operation used in the present invention are known in the art unless otherwise indicated.

Embodiment 1

[0124] Example 1 Hyaluronic acid (HA) modified loaded rosuvastatin (R) mesoporous silica nanocarrier (mSiO 2 - Preparation of (R)-HA)

[0125] Mesoporous / hollow nanosilicon spheres are made of SiO 2 Nanoparticles composed of materials with mesoporous or hollow structures. The mesoporous / hollow structure can be used to load hydrophilic, hydrophobic or amphiphilic drug molecules, and various targeted enrichment and microenvironmental stimulus-responsive drug release can be achieved by designing its outer surface. This patent selects mesoporous nano-silica spheres (mSiO 2 NPs) are drug carriers, loaded with the therapeutic drug rosuvastatin, and linked with hyaluronic acid (HA) targeting molecules on their surface to achieve targeted recognition and drug delivery.

[0126] 1.1 Mesoporous silica nanocarriers (mSiO 2 NPs) preparation

[0127]Reference Methods Synthesis of Hollow Mesoporous Silica Nanoparticles with Very Large Pores for Enhanced Adsorption and Release of DNA....

Embodiment 2

[0131] Example 2 selectin (SP) modified loaded rosuvastatin (R) mesoporous silica nanocarrier (mSiO 2 - Preparation of (R)-SP)

[0132] Prepare mSiO by a kind of method of embodiment 1 2 Nanocarriers, 1mg mL -1 Rosuvastatin (R)DMSO solution was added dropwise to mSiO 2 NP solution (10mg mL -1 PBS, pH=7.4), after ultrasonication for 30 minutes to mix evenly, stir at room temperature for 24 hours. The nano-drug carriers were precipitated by centrifugation, the supernatant was removed and the pellet was lyophilized to obtain mSiO 2 -(R) nano drug carrier.

[0133] Fully dissolve 1mg of selectin (SP) in PBS buffer solution, add 0.5mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) and 0.5mg of N - Hydroxysulfosuccinimide (sulfo-NHS) coupling reagent activates the carboxyl group. After stirring the reaction at room temperature for 1 hour, the unreacted small organic molecules were purified by ultrafiltration. Prepare the activated SP to 1 mg mL -1...

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Abstract

A mesoporous / hollow silica nanocarrier delivery system for targeting activated CD44 molecules, the surface of the nanocarrier is partially modified by a targeting ligand capable of interacting with activated CD44 A ligand to which a molecule specifically binds. A preparation method and application of the mesoporous / hollow silica nanocarrier delivery system. The mesoporous / hollow silica nanocarrier delivery system can be used for the diagnosis, prevention and treatment of vulnerable plaques or diseases related to vulnerable plaques.

Description

[0001] Cross References to Related Applications [0002] This application claims the priority of the Chinese invention patent application No. CN201810060265.9 submitted on January 22, 2018, which is incorporated herein by reference. technical field [0003] The invention belongs to the technical field of targeted drug delivery, and in particular relates to a nano-carrier for targeting and activating CD44 molecules, especially for targeting vulnerable plaques, especially a mesoporous / hollow silica nano-delivery system. The present invention also relates to the preparation and use of said nanocarriers, especially mesoporous / hollow silica delivery systems, especially in the diagnosis, prevention and treatment of vulnerable plaques or diseases associated with vulnerable plaques use. Background technique [0004] At present, acute cardiovascular events, mainly acute myocardial infarction and sudden cardiac death, have become the number one killer of human health. According to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K47/04A61P7/00A61P9/00
CPCA61P7/00A61P9/00A61K51/1234A61K49/1812A61K49/0409A61K47/6911A61K47/6913A61K9/1271A61K9/1272A61K9/0019A61K9/127A61K47/10A61K47/42A61K47/36A61P9/10A61K49/0002A61K49/0084A61K49/04A61K45/06B82Y5/00
Inventor 马茜孙洁芳
Owner BEIJING INNO MEDICINE CO LTD
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