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Preparation method and culture medium of binary acid diaminopentane salt

A dibasic acid ammonium salt, dibasic acid technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of reducing treatment work and costs, having a lot of pressure, generating a large amount of inorganic salts, etc. problem, to reduce environmental damage, less inorganic ion content, and meet the effect of the preparation process

Pending Publication Date: 2019-12-06
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that a large amount of inorganic salts will be produced in the process of preparing pentamethylenediamine by biological fermentation, which will put a lot of pressure on the subsequent treatment, the present invention provides a variety of environment-friendly culture media especially for the fermentation of pentamethylenediamine, and the use of the culture medium Method for preparing dibasic acid pentamethylenediamine salt by fermentation
The method for preparing dibasic acid pentamethylenediamine salt by fermentation of the present invention avoids the problem of producing a large amount of inorganic ions in the process of preparing pentamethylenediamine by the existing biological fermentation method, thereby avoiding the subsequent treatment process of inorganic ions and greatly reducing the subsequent The processing work and cost reduce the damage of inorganic ions to the environment, and the obtained dibasic acid pentamethylenediamine salt can be directly polymerized to obtain a specific polymer after treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0075] Mainly use three kinds of medium in the specific embodiment:

[0076] 1. Seed medium

[0077] Contains the following ingredients: KH 2 PO 4 0.2-0.5%, MgSO 4 ·7H 2 O 0.15-0.45%, MnSO 4 ·H 2 O 10-20ppm, dibasic acid ammonium salt 0.1%-2.5%, glucose 5%-15%, corn steep liquor 0.05%-0.30%, threonine 0.015-0.045%, leucine 0.010-0.025%;

[0078] 2. Fermentation medium

[0079] Contains the following ingredients: KH 2 PO 4 0.02%-0.06%, MgSO 4 ·7H 2 O 0.10%-0.50%, MnSO 4 ·H 2 O0.010%-0.050%, dibasic acid ammonium salt 0.1%-1.0%, glucose 1.0%-6.0%, corn steep liquor 0.10%-0.50%, threonine 0.010%-0.030%;

[0080] 3. Feeding medium:

[0081] The following ingredients are included: glucose 10%-80%, dibasic acid ammonium salt 5%-60%.

[0082] Fermentation culture method:

[0083] (1) Seed tank cultivation: 10L fermenter (working volume 4-7L), inoculum ratio 0.5-2%, aeration ratio 0.4-0.8vvm, temperature 35-39°C, speed 300-800rpm, tank pressure 0.05-0.15MPa, Control...

preparation Embodiment 1

[0087] Preparation of ammonium salt of dodecanedibasic acid

[0088] 1. Strain activation:

[0089] Take the glycerol tube strain of Candida and inoculate it in a seed bottle equipped with YPD culture, at natural pH, at 29°C, 220rpm, amplitude 26-50mm shaker culture for 1 day; YPD medium includes: 20g / L glucose, 10g / L yeast extract powder, 20g / l peptone; pH7.0-7.5;

[0090] 2. Cultivate in seed tanks and prepare seed solution:

[0091] Take the seeds from the seed bottle and put them into the seed tank with the seed medium. The initial pH value of the fermentation system after inoculation is 6.0. At 29°C, the ventilation ratio is 0.5vvm, and the tank pressure is 0.1MPa. Dissolved oxygen in the cultivation process is greater than or equal to 10%, cultivated for 18 hours, and the standard for cultivating mature seeds is OD620 of 15 after dilution of 30 times;

[0092] Seed medium includes: 20g / L sucrose, 8g / L corn syrup, 5g / L yeast extract, 8g / L KH 2 PO 4 , 3g / L urea, 15mL...

preparation Embodiment 2

[0098] Preparation of ammonium sebacate

[0099] 1. Strain activation:

[0100] Take the glycerol tube strain of Candida and inoculate it into the seed bottle with YPD culture, the pH is natural, at 29°C, 220rpm shaker culture for 1 day; YPD medium includes: 20g / L glucose, 10g / L yeast extract powder , 20g / l peptone; pH7.0;

[0101] 2. Cultivate in seed tanks and prepare seed solution:

[0102] Take the seeds from the seed bottle and put them into the seed tank with the seed medium. The initial pH value of the fermentation system after inoculation is 6.0. At 29°C, the ventilation ratio is 0.5vvm, and the tank pressure is 0.1MPa. Dissolved oxygen in the cultivation process is greater than or equal to 10%, cultivated for 18-20 hours, and the standard for cultivating mature seeds is OD620 of 15 after dilution of 30 times;

[0103] Seed medium includes: 20g / L sucrose, 8g / L corn syrup, 5g / L yeast extract, 8g / L KH 2 PO 4 , 3g / L urea, prepared with water, and sterilized at 121°C ...

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Abstract

The invention discloses a preparation method and a culture medium of a binary acid diaminopentane salt. In the fermentation culture medium, a binary acid ammonium salt serves as a nitrogen source, andfurther serves as a substrate to be added in a mode of supplementing the culture medium. In the fermentation process, the inoculation amount of fermentation is 5-20%, the temperature is 35-39 DEG C,and the ventilation quantity is 0.4-0.8 vvm; and / or the tank pressure is 0.05-0.2 MPa, the pH value is 6-7, and after fermentation for 2-6 h, the culture medium is subjected to fed-batch cultivation.According to the method, generation of a large number of inorganic ions in a fermentation system is avoided, subsequent treatment work and the subsequent treatment cost are greatly reduced, and damageof the inorganic ions to the environment is reduced.

Description

technical field [0001] The invention relates to an environment-friendly culture medium and a method for preparing dibasic acid pentamethylenediamine salt by fermentation of the culture medium. Background technique [0002] Diaminopentane (DN5), also known as cadaverine, is widely used in agriculture, medicine and industry. In agriculture, exogenous application of pentamethylenediamine can improve fruit setting and promote fruit ripening; in medicine, pentamethylenediamine can be used as an effective drug for treating dysentery; in industry, pentamethylenediamine prepared by biological fermentation can replace Diamines from petrochemical products undergo polymerization reactions to produce various polymers with different properties, thereby avoiding the disadvantages of high cost of petrochemical products and adverse effects on the environment, and can be widely used in aerospace, automotive parts, machinery Parts, electronic appliances and packaging materials and other fiel...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/72
CPCC12P13/001
Inventor 路飞徐敏刘修才
Owner CATHAY R&D CENT CO LTD
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