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Preparation method of EPA-containing nannochloropsis oil

A technology for Nannochloropsis algal oil and Nannochloropsis dry algal powder, which is applied in the directions of fat oil/fat production, fat production, etc., can solve the problem of low extraction rate, affecting the appearance of EPA products, and black color of EPA-containing algal oil, etc. problem, to achieve the effect of improving the extraction rate

Inactive Publication Date: 2019-12-06
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine microalga Nannochloropsis is rich in EPA, but the EPA-rich crude algae oil extracted from microalgae contains more impurities and pigments, which affect the appearance of EPA products and reduce the quality level of EPA products
[0003] At present, the methods for extracting EPA algae oil at home and abroad mainly include organic solvent extraction, pressing, CO 2 Supercritical extraction method, etc., but the above-mentioned extraction process is limited by the technical conditions of the existing extraction process conditions, the extracted EPA-containing algae oil is black in color, and the extraction rate is low

Method used

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preparation example Construction

[0017] The invention provides a method for preparing EPA-containing Nannochloropsis algae oil, comprising the steps of:

[0018] Step 1. Centrifuge the Nannochloropsis cell culture solution in a centrifuge with a rotating speed of 4000-8000 r / min for 20-40 minutes to obtain a Nannochloropsis micro-dehydration liquid. The water content of the Nannochloropsis micro-dehydration liquid is The range is 2~10%;

[0019] Wherein, the preparation method of the Nannochloropsis cell culture solution is as follows: NaCl with a concentration of 20.0 g / L, KCl with a concentration of 0.6 g / L, and NaCl with a concentration of 4 g / L 2 SO 4 , NaHCO with a concentration of 0.2g / L 3 , KBr with a concentration of 0.1g / L, and H with a concentration of 0.02g / L 3 BO 3 , NaF with a concentration of 0.004 g / L, and MgCl with a concentration of 10 g / L 2 , a concentration of 1.5 g / L CaCl 2 Mixture, under the light of 3000~10000 lux and the temperature of 25-30 ℃, pass through the 2 Concentration of...

Embodiment 1

[0029]The Nannochloropsis cell culture solution was centrifuged in a centrifuge at a speed of 4000 / min for 40 minutes to obtain a Nannochloropsis micro-dehydration liquid, and the water content range of the Nannochloropsis micro-dehydration liquid was 10%; The micro-dehydration liquid of Nannochloropsis is spray-dried, and the spray-drying conditions are as follows: the air inlet temperature is 120°C, and the air outlet temperature is 60°C to obtain Nannochloropsis dry algae powder; the Nannochloropsis dry algae powder is mixed with The decolorization solution was mixed at a weight ratio of 0.5:30, and reacted at 30°C for 240 minutes to obtain the Nannochloropsis decolorization solution; the Nannochloropsis decolorization solution was evaporated at a temperature of 40°C and a pressure of -0.12Mpa in a low-temperature vacuum for 6 Remove ethanol within 1 hour to obtain Nannochloropsis decolorized dry algae powder; perform subcritical extraction on the Nannochloropsis decolorized...

Embodiment 2

[0031] Centrifuge the Nannochloropsis cell culture solution in a centrifuge with a rotating speed of 8000 r / min for 20 minutes to obtain a Nannochloropsis micro-dehydration liquid, the water content of the Nannochloropsis micro-dehydration liquid is 2%; The micro-dehydration liquid of Nannochloropsis is spray-dried, and the spray-drying conditions are as follows: the air inlet temperature is 160°C, and the air outlet temperature is 80°C to obtain Nannochloropsis dry algae powder; the Nannochloropsis dry algae powder Mix with the decolorizing solution at a weight ratio of 5.0:50, react at 80°C for 60 minutes to obtain the Nannochloropsis decolorizing solution; evaporate the Nannochloropsis decolorizing solution at a temperature of 50°C and a pressure of -0.05Mpa in a low-temperature vacuum Remove ethanol for 4 hours to obtain Nannochloropsis decolorized dry algae powder; carry out subcritical extraction of the Nannochloropsis decolorized dry algae powder: the weight ratio of Nan...

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Abstract

The invention discloses a preparation method of eicosapentaenoic acid (EPA)-containing nannochloropsis oil. The method includes the following steps: step 1, performing centrifugation on a nannochloropsis cell culture liquid in a centrifuge at a rotating speed of 4,000 to 8000 r / min for 20-40 min to obtain a nannochloropsis microdehydrated solution; step 2, performing spray drying treatment on thenannochloropsis microdehydrated solution to obtain dried nannochloropsis powder; step 3, mixing the dried nannochloropsis powder and a decoloring solution, and performing a reaction at 30-80 DEG C for60-240 min to obtain a nannochloropsis decolorized solution; step 4, performing low-temperature vacuum evaporation on the nannochloropsis decolorized solution to remove ethanol to obtain nannochloropsis decolorized dried powder; and step 5, performing subcritical extraction on the nannochloropsis decolorized dried powder to obtain the EPA-containing nannochloropsis oil. The preparation method ofthe EPA-containing nannochloropsis oil is safe in process and convenient in preparation.

Description

technical field [0001] The invention relates to the technical field of Nannochloropsis algae oil, in particular to a preparation method of Nannochloropsis algae oil containing EPA. Background technique [0002] Eicosapentaenoic acid (EPA) is an important ω-3 long-chain polyunsaturated fatty acid. It has remarkable effects in preventing human metabolic disorders, arteriosclerosis, cardiovascular embolism, lowering blood pressure and cholesterol, and inhibiting platelet aggregation. Its importance to the human body has been clearly verified in clinical and epidemiological studies. Most of the EPA products currently on the market are derived from deep-sea fish oil, which have disadvantages such as high cholesterol content and strong fishy smell that affects the taste. Marine microalgae are the initial producers of EPA and other polyunsaturated fatty acids (Polyunsaturated fatty acids PUFAs). Compared with fish oil, the composition of fatty acids in microalgae is more stable an...

Claims

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Application Information

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IPC IPC(8): C11B1/04C11B1/10
CPCC11B1/04C11B1/10
Inventor 郑竟成田华雷芬芬何东平贺瑶曹健赵康宇
Owner WUHAN POLYTECHNIC UNIVERSITY
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