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Method for preparing thymosin injection

A technology of injection and thymosin, which is applied in the field of biological product preparation, can solve the problems of ineffective removal of miscellaneous proteins, endotoxins and exogenous viruses, destruction of active ingredients of raw materials, and low utilization rate of active ingredients, so as to achieve removal of exogenous viruses and exogenous viruses. Effects of endotoxin, exogenous virus and endotoxin, shortening production cycle

Pending Publication Date: 2019-11-26
SHANDONG SINDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Thymosin injection is produced by traditional production methods. The raw materials are made by pretreatment, mincing, homogenization, freeze-thawing, concentration, ultrafiltration membrane ultrafiltration and other processes. Traditional production processes cannot effectively remove impurities, endotoxins and exogenous viruses, etc. , will destroy the effective active ingredients of raw materials, the utilization rate of active ingredients is low, and the production cycle is long

Method used

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  • Method for preparing thymosin injection
  • Method for preparing thymosin injection
  • Method for preparing thymosin injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method for veterinary thymosin injection, the specific steps are as follows:

[0032] S1: Collection and inspection of calf thymus

[0033] Raw materials are selected from standardized raw cattle breeding and slaughtering companies to collect calf thymus. The breed of calf used for collecting calf thymus should be meat-eating fattening cattle, and the calf should be younger than 24 months when slaughtered. Slaughtering enterprises should have the "Animal Epidemic Prevention Certificate", the slaughterhouse should comply with the provisions of GB / T19477-2004 "Operational Regulations for Cattle Slaughter" and NY467-2001 "Standards for Sanitary and Quarantine of Livestock and Poultry Slaughter", and the hygiene requirements should comply with GB12649 "Meat Processing Factory Hygienic Standards.

[0034] Calf thymus can be used as raw material only after passing the appearance inspection, pathogenic microorganism inspection, and exogenous virus inspection. F...

Embodiment 2

[0047] A preparation method for veterinary thymosin injection, the specific steps are as follows:

[0048] S1: Collection and inspection of calf thymus

[0049] Raw materials are selected from standardized raw cattle breeding and slaughtering companies to collect calf thymus. The breed of calf used for collecting calf thymus should be meat-eating fattening cattle, and the calf should be younger than 24 months when slaughtered. Slaughtering enterprises should have the "Animal Epidemic Prevention Certificate", the slaughterhouse should comply with the provisions of GB / T19477-2004 "Operational Regulations for Cattle Slaughter" and NY467-2001 "Standards for Sanitation and Quarantine of Livestock and Poultry Slaughter", and the hygiene requirements should meet the requirements of GB12649 "Meat Processing Factory Hygienic Standards.

[0050]Calf thymus can be used as raw material only after passing the appearance inspection, pathogenic microorganism inspection, and exogenous virus ...

Embodiment 3

[0063] A preparation method for veterinary thymosin injection, the specific steps are as follows:

[0064] S1: Collection and inspection of calf thymus

[0065] Raw materials are selected from standardized raw cattle breeding and slaughtering companies to collect calf thymus. The breed of calf used for collecting calf thymus should be meat-eating fattening cattle, and the calf should be younger than 24 months when slaughtered. Slaughtering enterprises should have the "Animal Epidemic Prevention Certificate", the slaughterhouse should comply with the provisions of GB / T19477-2004 "Operational Regulations for Cattle Slaughter" and NY467-2001 "Standards for Sanitary and Quarantine of Livestock and Poultry Slaughter", and the hygiene requirements should comply with GB12649 "Meat Processing Factory Hygienic Standards.

[0066] Calf thymus can be used as raw material only after passing the appearance inspection, pathogenic microorganism inspection, and exogenous virus inspection. F...

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Abstract

The invention belongs to the technical field of biological product preparation, and particularly relates to a method for preparing a thymosin injection. A calf thymus which passes inspection is removed of inner and outer packaging, and after pretreatment, primary mincing is conducted for 1 to 3 times; the primarily minced calf thymus is subjected to fine mincing and grinding for 3 to 8 minutes tobe prepared into homogenate; the homogenate is subjected to supersonic decomposing in an ultrasonic crushing device, the pH value of a lysate solution is adjusted with an HCl solution to 2.0 to 2.5; the lysed slurry is placed in a cold storage at -20 DEG C for freeze thawing for 1 to 2 times; after the last freeze thawing, heating is conducted to 80 DEG C + / - 2 DEG C, the temperature is kept for 10 to 20 minutes, a filter cloth is used for filtering, and an extraction solution is prepared; the extraction solution is subjected to centrifugal separation, filtration, and ultra filtration to obtain a semi-finished product; and water for injection is added to the qualified semi-finished product to obtain a finished product. The method for preparing the thymosin injection has the advantages thatthe technology is simple, the cost is low, the production cycle can be greatly shortened, manpower and material resources are saved, the production efficiency of thymosin injection is improved, and the application prospect is wider.

Description

technical field [0001] The invention belongs to the technical field of preparation methods of biological products, in particular to a preparation method of thymosin injection. Background technique [0002] At present, under the condition of increasing food nutrition and safety requirements and increasing awareness of environmental protection, it is urgent to seek ways to improve animal body endocrine to promote growth, improve carcass quality and flavor, and then solve the problem of using additives to promote growth and meat quality decline, and drug residues. The effective way of the contradiction. [0003] Thymosin is a kind of polypeptide hormone secreted by epithelial cells of thymus tissue, which can promote the maturation of T cells, participate in the interaction between neuroendocrine system and immune system, and activate cellular immunity. Since Roberts first used calf thymus tissue to prepare thymosin crude liquid in 1949, many scholars at home and abroad have d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/22A61K9/08A61P31/12A61P35/00C07K14/575C07K1/14C07K1/34
CPCA61K38/2292A61K9/08A61K9/0019A61K47/02A61P35/00A61P31/12C07K14/57581
Inventor 薛希娟乔彦良王学波李朝阳
Owner SHANDONG SINDER TECH
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