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Method for separating and purifying main carotenoids in orange peel

A technology for separation and purification of carotene, which is applied in the field of separation and purification of main carotenoids in orange peel, can solve the problems of single species, low purity, and inability to obtain high-purity carotenoid monomer components, etc., and achieves a simple and practical method, good separation effect

Inactive Publication Date: 2019-11-22
SOUTHWEST UNIVERSITY
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Problems solved by technology

[0005] The current research on the separation and purification of carotenoids from orange peel usually adopts a single separation procedure of silica gel column chromatography (OCC). Carotenoid component analysis cannot obtain high-purity carotenoid monomer components

Method used

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  • Method for separating and purifying main carotenoids in orange peel
  • Method for separating and purifying main carotenoids in orange peel
  • Method for separating and purifying main carotenoids in orange peel

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Embodiment

[0058] 1. Materials, Reagents and Instruments

[0059] 1.1 Materials: extract to obtain carotenoid saponification extract.

[0060] 1.2 Reagents and instruments

[0061] Table 1 Experimental Reagents

[0062]

[0063] Table 2 Experimental Instruments

[0064]

[0065] 2. Experimental method

[0066] 2.1 Separation and purification process of carotenoids: saponified orange peel carotenoids (SCE) prepared by the optimized microwave saponification method, the separation and purification process of carotenoids is as follows figure 2 shown.

[0067] 2.2 Acetone precipitation: The acetone precipitation method can effectively remove some neutral lipid impurities in the crude carotenoid extract and enhance the subsequent chromatography efficiency. Concentrate the pigment concentrate obtained in 2.1 to dryness under reduced pressure and weigh it, add acetone solution at 20 mL / g, place at -80°C for 12 hours, centrifuge to remove the precipitate, and concentrate the supernata...

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Abstract

The invention belongs to the technical field of separation and purification. The invention discloses a separation and purification method of main carotenoids in orange peel. An orange peel carotenoidsaponification extract liquid is used as a raw material; acetone precipitation-silica gel column chromatography-magnesium oxide column chromatography / semi-preparative liquid phase-crystallization andother means are combined to purify the orange peel carotenoids to obtain crystals of four high-purity carotenoids which are R1, R2, R3 and R4; and the purified carotenoids obtained are subjected to structure identification through thin-layer chromatography, a C30 column, high performance liquid chromatography-diode array detection-atmospheric pressure chemical ionization tandem mass spectrometry and nuclear magnetic resonance spectrometry. The purities of beta-carotene, beta-cryptoxanthin, xanthophyll and zeaxanthine obtained by the method reach 98.79%, 99.11%, 96.59% and 96.77% respectively,and the yields are 0.0025 per mill, 0.0275 per mill, 0.0066 per mill and 0.0092 per mill respectively.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of main carotenoids in orange peel, and in particular relates to a method for separation and purification of main carotenoids in orange peel. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Since natural carotenoids to be applied in the fields of food, cosmetics and pharmaceuticals usually have certain purity requirements, separation and purification steps after carotenoid extraction are essential. At present, the separation and purification techniques of plant-derived carotenoids mainly include thin-layer chromatography (TLC), column chromatography (OCC), preparative or semi-preparative liquid chromatography (HPLC), and crystallization. The TLC method is only suitable for the separation of a small amount of samples (the sample amount is less than 1mg); the column chromatography (OCC) method is the pref...

Claims

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Application Information

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IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 孙志高方明马亚琴黄林华郭莉谭祥
Owner SOUTHWEST UNIVERSITY
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