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Tilapia feed additive capable of reducing proinflammatory response and enhancing immune response and preparation method and application thereof

A technology for enhancing immune response and feed additive, applied in the field of tilapia feed additive and its preparation, can solve problems such as excessive excretion and water pollution, and achieve the effects of no muscle residue, promoting immune response and reducing expression.

Active Publication Date: 2019-11-12
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tilapia eats vigorously in summer and excretes a lot, causing serious water pollution

Method used

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  • Tilapia feed additive capable of reducing proinflammatory response and enhancing immune response and preparation method and application thereof

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preparation example Construction

[0025] The invention provides the preparation method of described tilapia feed additive, comprises the following steps:

[0026] 1) mixing Acanthopanax stalks, loquat leaves, tripterygium wilfordii, hawthorn and mulberry leaves, decocting with water to obtain a decoction;

[0027] 2) Concentrating the decoction liquid, carrying out alcohol precipitation, solid-liquid separation, and collecting the liquid phase to obtain the feed additive.

[0028] The invention mixes the stems of Acanthopanax senticosus, loquat leaves, tripterygium wilfordii, hawthorn and mulberry leaves, and decocts with water to obtain decoction.

[0029] In the present invention, the volume ratio of the total mass of Chinese medicinal materials to water is preferably 0.8-1.2g:0.8-1.2mL, more preferably 1g:1mL. The decocting temperature is preferably 90-100°C, more preferably 95°C. The decocting time is preferably 1-2 hours, more preferably 1.5 hours. After decoction, it is preferred to perform solid-liqu...

Embodiment 1

[0038] A method for preparing a feed additive for reducing the pro-inflammatory response and enhancing the immune response of tilapia adult fish in water tank culture, comprising the following steps:

[0039] Accurately weigh 30g of Acanthopanax stem, 20g of loquat leaves, 20g of Tripterygium wilfordii, 15g of hawthorn and 15g of mulberry leaves. After mixing, add water according to the ratio of 1L of water per 1kg of Chinese herbal medicine, cook at 95°C for 1.5h, and separate the solid and liquid. Collect the aqueous phase. Dry the water in an oven at 55°C for 3 hours to obtain a concentrated solution. The concentrated solution was alcohol-precipitated with 95% ethanol. Suction filtration, and the filtrate was recovered under reduced pressure at 60°C and -0.08Mpa to obtain extract. The obtained extract was dried in an oven at 45°C for 2 hours, and then crushed into dry powder.

Embodiment 2

[0041] A set of feed compatibility for reducing the pro-inflammatory response and enhancing the immune response of tilapia adult fish in flow tank culture and its implementation method include the following steps:

[0042] Accurately weigh 30g of Acanthopanax stem, 20g of loquat leaf, 20g of Tripterygium wilfordii, 15g of hawthorn and 15g of mulberry leaf. The above-mentioned Chinese herbal medicines were mixed with water and decocted, and 1L of purified water was added to every 1kg of Chinese herbal medicines, and steamed at 100°C for 1.0h. Dry the water in an oven at 55° C. for 3 hours to obtain a concentrated solution. According to the volume ratio of the aqueous ethanol solution to the concentrated solution of 4:1, add an appropriate amount of 95% ethanol to the alcohol precipitation tank to carry out alcohol precipitation on the concentrated solution. Suction filtration (the pore diameter range of the suction filtration membrane is 3 μm), and the filtrate is recovered un...

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Abstract

The invention provides a tilapia feed additive for reducing proinflammatory response and enhancing immune response and a preparation method and an application thereof, and belongs to the technical field of aquaculture. The tilapia feed additive for reducing proinflammatory response and enhancing immune response comprises the following components in parts by weight: 25-35 parts of manyprickle acanthopanax stems, 15-25 parts of loquat leaves, 15-25 parts of tripterygium wilfordii, 10-20 parts of hawthorn fruits and 10-20 parts of mulberry leaves. The invention also provides a tilapia feed containing the tilapia feed additive, and an application of the tilapia feed additive or the tilapia feed in tilapia breeding. The application can effectively reduce the proinflammatory response of the headkidney tissue and the spleen tissue of the tilapia, and enhance the immune response capability of the tilapia, thereby improving the capability of the tilapia against streptococcal infection.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, relates to the technical field of aquaculture management and feed feeding, in particular to a tilapia feed additive for reducing pro-inflammatory response and enhancing immune response, and a preparation method and application thereof. Background technique [0002] Flowing tank recirculating aquaculture is to change the "open free-range culture" of traditional ponds into "intensive captive culture", and realize "flowing water" fish farming in "still water" ponds. Through mechanical waves and currents, an annular water flow is formed in the entire large pond. The water flows through the water tank, which can centrally feed aquatic products in the "runway", and use the water flow to concentrate excreta in a tank for unified treatment, thereby Play the role of purifying pond water quality. Environmental stress changes the body's non-specific immunity, leading to inflammation and cell damage in ...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A23K40/10A01K61/13A61K36/734A61P29/00A61P37/04A61P31/04
CPCA23K50/80A23K10/30A23K40/10A01K61/13A61K36/254A61K36/73A61K36/37A61K36/734A61K36/605A61P29/00A61P37/04A61P31/04A61K2236/331A61K2236/51A61K2236/53A61K2300/00Y02A40/81
Inventor 强俊何杰徐跑陶易凡包景文朱昊俊曹哲明
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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