Hybridoma cell strain GTY for secreting anti-isoprocarb monoclonal antibody and application thereof

Abstract

The invention relates to a hybridoma cell strain GTY for secreting an anti-isoprocarb monoclonal antibody and an application thereof, and belongs to the field of food safety immune detection. The hybridoma cell strain GTY has been preserved in the China General Microbiological Culture Collection Center with a preservation number CGMCC No.17393. The complete antigen of isoprocarb and the same amount of Freund's adjuvant are mixed and emulsified to immunize BALB / c mice. The first immunization uses complete Freund's adjuvant, the multiple reinforcement immunization uses incomplete Freund's adjuvant, and the last sprint immunization is carried out. The hybridoma cell strain GTY is prepared by the following steps of: fusing high-potency low-IC50 mouse spleen cells with mouse myeloma cells by aPEG method, and performing culturing and screening; and screening cells by indirect competitive enzyme-linked immunosorbent assay, and performing subcloning three times. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity on the isoprocarb, can realize the detection of the isoprocarb residue in fruits and vegetables, provides raw materials for the immunodetection of the isoprocarb residue in foods, and has practical application value.

Description

technical field [0001] The invention relates to a hybridoma cell line GTY secreting anti-isoprocarb monoclonal antibody and application thereof, which belongs to the field of food safety immune detection. Background technique [0002] Isoprocarb, also known as yechansan and mepocarb, is a carbamate pesticide. Because of its stomach poisoning, contact killing and fumigation effects, it is widely used to control rice pests and has been widely used all over the world. However, it is toxic to both animals and humans. In recent years, due to a large number of unscientific use, human and animal poisoning has occurred from time to time, so many countries and regions have set strict limits on the residues of this pesticide in fruits and vegetables. The analytical methods commonly used for the determination of carbamate pesticides include high performance liquid chromatography (HPLC), gas chromatography (GC) and HPLC-MS. Each method has its advantages, but also disadvantages. For ...

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Problems solved by technology

For example, although these methods have good selectivity and high sensitivity, the analysis time is long, the consumption of organic solvents is large, and the instruments used are expensive.

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