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Paenibacillus peoriae and application thereof

A technology of bacillus and bacterial wilt, applied in the application, bacteria, fungicides and other directions, can solve the problems of chemical prevention and control of environmental hazards, tomato bacterial wilt outbreak, not suitable for long-term use, etc., to achieve human and animal safety, resistance to drug resistance, The effect of strong heat resistance and stress resistance

Active Publication Date: 2019-11-08
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tomato planting area is constantly expanding. Once infected with bacterial wilt, the number of bacterial wilt bacteria in the soil will continue to accumulate, especially in the southern region, where the humid and rainy climate provides conditions for the spread of the disease, resulting in a large-scale outbreak of tomato bacterial wilt. Under normal circumstances, the loss can reach 10% to 30%, and in severe cases, it can cause failure.
[0005]Chemical control methods are currently used for the two diseases, but chemical control will have harmful effects on the environment and should not be used for a long time

Method used

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  • Paenibacillus peoriae and application thereof
  • Paenibacillus peoriae and application thereof
  • Paenibacillus peoriae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Identification of Paenibacillus Peirui

[0027] (1) Isolation of strains

[0028] The strain was isolated from grassland soil in Tibet. Use a 50mL centrifuge tube to weigh 5g of soil sample, add 45mL of 0.1% sterile Tween water, mediate and shake for 10min, then let it stand for 30min until the soil particles settle down to the bottom of the centrifuge tube, and dilute the suspension gradient to 10 -2 、10 -3 、10 -4 Concentration, coated on LB medium plate, each gradient coated three plates. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium were picked and streaked and purified on the LB medium plate, and the growth of the colonies was observed regularly.

[0029] (2) Identification of strains

[0030] Using a single colony as a template, the amplification primers are 16SrRNA universal primer 27F:

[0031] 5'-AGAGTTTGATCCTGGCTCAG-3 (shown in SEQ ID NO: 2) and 1492R:

[0032] 5'-GGTTACCTTGTTACGACT...

Embodiment 2

[0034] Embodiment 2 Pear Rui Russian paenibacillus culture method

[0035] (1) Solid LB medium is used for laboratory solid culture.

[0036] (2) Laboratory liquid medium: 30g sucrose, 1.2g potassium nitrate, 1g disodium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g beef extract, distilled water to 1L, pH7.2~7.5, high temperature sterilization; Culture conditions 37° C. 200 rpm.

Embodiment 3

[0037] Example 3 Determination of the Effect of Paenibacillus Peirui on Controlling Cucumber Blight

[0038] (1) Preparation of Paenibacillus fermented liquid

[0039] Pick a single colony and insert it into the above liquid medium, culture it in a shaker flask at 37°C and 200 rpm for 3 days, take it out and count the number of bacteria in the fermentation broth.

[0040] (2) Pot experiment

[0041] The experimental treatment agents include: clear water (blank control), 1000-fold diluted thiophanate-methyl solution (chemical drug), and Paenibacillus perryus fermentation broth.

[0042] Pot test method: indoor potting of cucumber seedlings was carried out in plastic pots with a caliber of 10 cm, 3 plants were planted in each pot, 30 plants in total for each treatment, and repeated three times. When the melon seedlings grew to 7 days, the plants with the same growth were selected for the test, and each plant was irrigated with 2mL of the treatment agent. After the first appli...

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Abstract

The invention relates to paenibacillus and application thereof, in particular to Paenibacillus peoriae and application thereof. The Paenibacillus peoriae is deposited to the Guangdong MicrobiologicalCulture Collection Centeron on November 16, 2018, and the preservation number is GDMCC No.60482. The Paenibacillus peoriae has a good prevention and treatment effect on pathogenic fungi and bacteria,especially can be used for inhibiting rhizoctonia solani and rastonia solanacearum, and has the advantages of high growth speed, simple nutrition demand, high heat resistance and stress resistance andthe like; and the Paenibacillus peoriae is safe for human and livestock, poisonless and harmless, environmentally friendly and free of pollution, and the pathogenic bacteria are not prone to producing drug resistance, so that the requirements of people for green food are met, and guarantee for the sustainable development of agriculture is provided.

Description

technical field [0001] The invention relates to a Paenibacillus and application thereof, in particular to a Paenibacillus pierreus and the application thereof. Background technique [0002] my country is a big consumer of fruits and vegetables. Along with the demand for vegetables, more and more seasonal vegetables are put on people's tables. With the cultivation of vegetables, the occurrence of diseases of fruits and vegetables has become an inevitable problem. . Diseases will not only affect the appearance of vegetables, but also affect their growth, yield and quality. Therefore, the prevention and control of diseases is the key to cultivation. However, in order to control the harm of diseases, vegetable farmers continue to increase the use of chemical pesticides, resulting in serious pesticide residues in melon and fruit products. Using bacteria to treat bacteria instead of using medicines to reduce the use of chemical pesticides is an important aspect of agricultural dev...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/01
CPCA01N63/00C12R2001/01C12N1/205
Inventor 余昕彤王科晶李得盛
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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