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Immunogenic composition containing rabbit staphylococcal antigen, and preparation method and application thereof

A technology of rabbit staphylococcus and staphylococcus, which is applied in the field of veterinary biological products, can solve the problems of storage space, vaccine strain failure, and reduced protection rate, and achieve the effect of easy storage, high porosity, and guaranteed firmness

Active Publication Date: 2019-11-08
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the vaccines in the prior art are injections and freeze-dried powders, which are large in size and take up space for storage, and it is easy to cause the failure of the vaccine strain during storage, resulting in a reduction in the protection rate

Method used

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  • Immunogenic composition containing rabbit staphylococcal antigen, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] (1) Preparation of Rabbit Staphylococcus Antigens

[0030] 1) Resuscitate the intestinal bacteria and respiratory bacteria isolated and cultured from the liver and lungs of dead rabbits infected with Staphylococcus aureus as bacterial strains. After passing the pure bacteria inspection, inoculate the two bacterial strains into ordinary broth medium respectively, Incubate at 37°C for 20 hours to make a bacterial solution with a predetermined concentration;

[0031] 2) Use turbidimetry and ultraviolet spectrophotometry to determine the concentration of respiratory tract bacteria to be 300 million / mL, add formaldehyde solution at 0.3% of the amount of bacteria solution, and inactivate at 37°C for 20 hours; determine the concentration of intestinal bacteria to be 1 billion / mL mL, first bathed at 100°C for 2.5h, then add 0.3% formaldehyde solution to inactivate at 37°C for 20h;

[0032] 3) Inoculate the intestinal bacteria and respiratory bacteria treated in step 2) on the ...

Embodiment 2

[0043] This embodiment is basically the same as Embodiment 1, the difference is:

[0044] The dispersion carrier in this embodiment is chitosan nanoparticles loaded with dispersin B. Disperse protein B can promote the overall diffusion of biofilm, and is beneficial to disperse and release rabbit staphylococcus antigenic strains.

[0045] The preparation method of the disperse carrier is as follows: add the dispersin B solution to the chitosan acetic acid solution with a concentration of 4.0mg / ml until the final concentration of the dispersin B is 2.0mg / ml, under the condition of 30-35℃ and magnetic stirring Mix well, let stand at 25°C for 22 hours, add phosphate ester with a final concentration of 1.5mg / ml under the condition of 200rpm low-speed magnetic stirring, to activate chitosan nanoparticle, and facilitate the loading of dispersin B, keep stirring for 35 minutes, The obtained chitosan nanoparticle loaded with dispersin B has the characteristic of firm loading of dispers...

Embodiment 3

[0051] This embodiment is basically the same as Embodiment 2, the difference is:

[0052] The aqueous printing adjuvant of the present embodiment adopts commercially available Montanide TM Gel's aqueous vaccine adjuvant.

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Abstract

The present invention discloses an immunogenic composition containing a rabbit staphylococcal antigen, and a preparation method and an application thereof. The immunogenic composition uses a mixture of a rabbit staphylococcal antigen, a dispersion carrier and an aqueous printing adjuvant as printing spray liquid and freeze-dried protective powder as accessory powder, and the printing spray liquidis repeatedly sprayed on each layer of the accessory powder by a 3D printing technique to obtain the immunogenic composition containing the rabbit staphylococcal antigen with a three-dimensional structure. The immunogenic composition prepared by the method has good immunogenic activity and immunoprotection, can be effectively used for prevention and treatment of rabbit staphylococcus aureus, and has an immune protection rate up to 100%, and the vaccine is good in stability and not liable to be inactivated.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an immunogenic composition containing rabbit staphylococcus antigen and its preparation method and application. Background technique [0002] Humans are the natural reservoir of Staphylococcus aureus (S.aureus). Healthy individuals can be continuously (10-35%), intermittently (20-75%) or in a non-carrier state (5-70%) colonized with S. aureus on the skin, nostrils and throat without associated disease. Large human reservoirs increase the chances of evolution and spread of adapted pathogenic clonal types. Of particular concern are invasive staphylococcal infections from the gram-positive cocci Staphylococcus aureus and S. epidermidis, as they are an increasing global public health problem. Specifically, Staphylococcus aureus is responsible for most nosocomial-acquired (hospital) infections, and its prevalence in community-onset infections is in...

Claims

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Application Information

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IPC IPC(8): A61K39/085A61K9/20A61K47/42A61K47/36A61P31/04
CPCA61K39/085A61K9/2063A61K9/205A61K9/2054A61P31/04A61K2039/552Y02A50/30
Inventor 孙晴刘言娟张桂芝葛艳艳王美欣
Owner LINYI UNIVERSITY
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