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Preparation and application of a same-energy rare earth luminescent probe based on change of luminescence lifetime

A technology of rare earth luminescence and fluorescence lifetime, which is applied in the direction of luminescent materials, fluorescence/phosphorescence, nanotechnology for materials and surface science, etc., and can solve problems such as failure to realize detection

Active Publication Date: 2019-10-25
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this patent application, the rare earth nano-luminescent materials excited and emitted at the same energy level are only used in imaging and tracking, but do not respond to the external environment, nor can they detect in simple or complex environments.

Method used

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  • Preparation and application of a same-energy rare earth luminescent probe based on change of luminescence lifetime
  • Preparation and application of a same-energy rare earth luminescent probe based on change of luminescence lifetime
  • Preparation and application of a same-energy rare earth luminescent probe based on change of luminescence lifetime

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: Nanoparticle NaYF 4 : Synthesis of Tm

[0095] Take 0.99mmol of YCl 3 and 0.01 mmol of TmCl 3 Add the solid powder to a 100mL three-neck flask containing 6mL oleic acid and 15mL octadecene, add a magnet and a thermometer to build an air condensation device. The temperature was raised to 110° C. under a nitrogen atmosphere, and then lowered to room temperature after the chloride was completely dissolved. While stirring, 10 mL of methanol solution dissolved with 4 mmol of ammonium fluoride and 2.5 mmol of sodium hydroxide was added dropwise into the reaction flask, and the reaction solution gradually became turbid. After stirring at room temperature for 15 minutes, the temperature was gradually raised to 110° C., and methanol was removed in an atmosphere of flowing nitrogen. Then, after removing the remaining low-boiling point substances by vacuuming, the temperature was rapidly raised to 300° C. for 1 hour under nitrogen atmosphere. After the reaction, tu...

Embodiment 2

[0097] Example 2: Nanoparticle NaYF 4 :Yb, NaYF 4 :Nd, NaYF 4 : Synthesis of Er

[0098] NaYF 4 :Yb, NaYF 4 :Nd, NaYF 4 : Synthesis steps of Er nanoparticles and NaYF 4 : The synthesis of Tm nanoparticles is the same, where TmCl 3 Solid powder replaced by YbCl 3 , NdCl 3 or ErCl 3 .

[0099] figure 2 is NaYF in this example 4 : The transmission electron micrograph of the Yb luminescent probe, the material size is 10nm, the material has good dispersion and uniform particle size.

[0100] image 3 is NaYF in this example 4 : Transmission electron micrograph of Nd luminescent probe, the material size is 6.5nm, the material has good dispersion and uniform particle size.

[0101] Figure 4 is NaYF in this example 4 : Transmission electron micrograph of Er luminescent probe, the material size is 38nm, the material has good dispersion and uniform particle size.

Embodiment 3

[0102] Example 3: Nanoparticle LiYF 4 :Nd, KYbF 3 , CaYbF 4 , BaYbF 4 Synthesis

[0103] YF 4 :Nd, KYbF 3 , CaYbF 4 , BaYbF 4 Nanoparticle synthesis steps and NaYF 4 : The synthesis of Tm nanoparticles is the same, where TmCl 3 Solid powder replaced by YbCl 3 or NdCl 3, replace NaOH solid with LiOH, KOH, Ca(OH) 2 or Ba(OH) 2 .

[0104] Figure 5 is LiYF in this example 4 : The transmission electron micrograph of the Nd luminescent probe, the material size is 31nm, the material has good dispersion and uniform particle size.

[0105] Image 6 is KYbF in this example 3 Transmission electron micrograph of the luminescent probe, the size of the material is 5nm, the material has good dispersion and uniform particle size.

[0106] Figure 7 is CaYbF in this example 4 Transmission electron micrograph of the luminescent probe, the size of the material is 10nm, the material has good dispersion and uniform particle size.

[0107] Figure 8 is BaYbF in this example ...

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Abstract

The invention relates to the technical field of bio-imaging, in particular to preparation and application of a same-energy rare earth luminescent probe based on change of luminescence lifetime. The rare earth luminescence lifetime probe comprises a luminescent donor Ln, an energy acceptor Qe and a linking material Link, the general structural formula is Ln-Link-Qe, and the luminescent donor is composed of rare earth nanoparticles, wherein energy absorption and fluorescence emission occur at the same electronic state energy level; the energy acceptor is matched with the luminescence wave band of the rare earth nanoparticles, and the energy transfer process occurs, so that the fluorescence lifetime is changed; and combination of the luminescent donor and the energy acceptor is performed through the linking material in an electrostatic adsorption, covalent coupling or twining mode, so that energy transfer is promoted. The luminescence process of the rare earth luminescent donor of the probe conforms to the first-order reaction kinetics, and the number of the energy acceptor of the probe relative to the luminescent donor is linear to the fluorescence lifetime of the rare earth material, so that the lifetime signal is utilized, and target detection in a complex environment is achieved; and great application prospects in the field of biological detection is achieved.

Description

technical field [0001] The invention relates to the technical field of biological imaging, in particular to the preparation and application of a rare earth luminescence probe of the same energy level based on the change of luminescence lifetime. Background technique [0002] Bioimaging technology has been widely used in many fields such as medical detection, including X-ray imaging, nuclear magnetic resonance imaging, fluorescence imaging and other technologies. Bioimaging technology has the advantages of non-invasiveness and intuitive results. Among them, fluorescence imaging technology occupies an important position in the field of biological imaging due to its advantages such as low cost, simple operation, and intuitive results. Therefore, a variety of fluorescent probes have been developed for in vivo and in vitro fluorescence imaging tracing and detection, such as organic dyes, inorganic quantum dots, carbon nanotubes, inorganic rare earth nanomaterials, etc. In order ...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/02C09K11/06C09K11/61B82Y30/00B82Y40/00G01N21/64
CPCC09K11/7773C09K11/7705C09K11/02C09K11/06C09K11/616B82Y30/00B82Y40/00G01N21/6486G01N21/6428G01N2021/6432
Inventor 李富友孔梦涯顾昱飏吴娜冯玮
Owner FUDAN UNIV
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