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Blood linezolid drug concentration monitoring kit and detection method thereof

A linezolid and kit technology, applied in the field of linezolid therapeutic drug monitoring kits in blood, can solve the problems of high technical requirements for experimenters, inaccurate qualitative and quantitative analysis, long analysis time, etc., and reduce errors and personnel operations The effect of error, simple and fast detection process, and reduced experimental cost

Inactive Publication Date: 2019-10-22
苏州艾迪迈医疗科技有限公司
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  • Claims
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Problems solved by technology

Previous methods have problems such as complicated pre-treatment, inaccurate qualitative and quantitative results, high cost, long analysis time, or high technical requirements for experimenters.

Method used

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  • Blood linezolid drug concentration monitoring kit and detection method thereof
  • Blood linezolid drug concentration monitoring kit and detection method thereof
  • Blood linezolid drug concentration monitoring kit and detection method thereof

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Embodiment Construction

[0035] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with illustrations and specific embodiments.

[0036] 1. Preparation of Linezolid Therapeutic Drug Monitoring Kit:

[0037] Standard curve reagent: Linezolid is prepared into a series of concentrations with animal serum containing 2% stabilizer and 2% plasticizer, respectively 0.3mg / L, 1.0mg / L, 2.0mg / L, 4.0mg / L, 8.0mg / L, 12.0mg / L, 1.0ml per bottle. The above components were freeze-dried and stored at the bottom of the assay bottle.

[0038] Quality Control Reagent: Linezolid is formulated with animal serum containing 2% stabilizer and 2% plasticizer to three concentrations of high, medium and low, respectively 0.5mg / L, 4.0mg / L, and 10.0mg / L. The bottle is filled with 1.0ml. The above components were freeze-dried and stored at the bottom of the assay bottle.

[0039] Samp...

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PUM

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Abstract

The invention relates to the field of medical detection and in particular to a blood linezolid therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquidchromatography analytical technology, more specifically to a method for accurately and quantitatively detecting linezolid drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. The therapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has various methodological indicators that satisfy the requirement for monitoring the blood linezolid therapeutic drugs, and is easy to generalize.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a linezolid therapeutic drug monitoring kit in blood developed based on multi-dimensional on-line solid-phase extraction liquid chromatography analysis technology, more specifically, an online solid-phase extraction Chromatographic analysis technology is a method for accurate and quantitative monitoring of linezolid drug concentration in blood. Background technique [0002] Linezolid is a synthetic oxazolidinone antibiotic used to treat infections caused by aerobic Gram-positive bacteria. Linezolid is a bacterial protein synthesis inhibitor, which acts on the bacterial 50S ribosomal subunit, inhibits the connection of mRNA to ribosomes, prevents the formation of the 70S initiation complex, and thus inhibits bacterial protein synthesis. The effective blood concentration of linezolid is 1.0-4.0mg / L, and when the blood concentration is higher than 4.0mg / L, the toxic and side effects ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/16G01N30/34G01N30/86
CPCG01N30/06G01N30/16G01N30/34G01N30/8631
Inventor 杨晓洪石功名何伶秀管燕
Owner 苏州艾迪迈医疗科技有限公司
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