Photosynthetic bacterial strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application thereof

A technology of photosynthetic bacteria and biocontrol agents, applied in biochemical equipment and methods, microbe-based methods, botany equipment and methods, etc., to achieve the effects of no phytotoxicity, short cultivation time, and environmental friendliness of crops

Active Publication Date: 2020-10-13
HUNAN PLANT PROTECTION INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Based on this, it is necessary to provide a photosynthetic bacterial strain, a biocontrol agent, a biocontrol fermentation liquid, a preparation method and an application for the prevention and treatment of citrus canker.

Method used

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  • Photosynthetic bacterial strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application thereof
  • Photosynthetic bacterial strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application thereof

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preparation example Construction

[0032] A preparation method for a photosynthetic bacteria biocontrol agent for the prevention and treatment of citrus canker, comprising the following steps:

[0033] Activation: culture the preserved species of photosynthetic bacteria strains according to the plate culture method, and cultivate them until a brown-red single colony appears;

[0034] Seed culture: insert maroon single colony into photosynthetic bacteria liquid medium for seed culture until logarithmic growth phase to obtain bacterial liquid;

[0035] Production culture: Bacteria liquid is inoculated to the photosynthetic bacteria production medium according to the inoculation amount of 5% of the total volume of the photosynthetic bacteria strains, and the photosynthetic bacteria biocontrol agent is obtained by carrying out production and cultivation until the logarithmic growth phase, such as figure 2 shown.

[0036] During activation, the preserved species of Rhodobacter capsularis A18 strain was cultured ac...

Embodiment 1

[0051] A photosynthetic bacterial strain named Rhodobacter capsularis A18 strain.

[0052] The main biological characteristics are: cultured on double-layer solid plate medium for 2d to 4d, a maroon circular colony is formed, the colony edge is neat and smooth, and the diameter of the colony is 0.2mm to 0.60mm; cultured in a liquid medium for 4d is brownish red; It grows well under aerobic conditions; the physiological and chemical characteristics are V-P reaction and methyl red reaction negative, H 2 S reaction, gelatin liquefaction, urease test, and indole test were all positive, and the optimum growth temperature was 28°C-32°C, pH=7.0.

Embodiment 2

[0054] A kind of photosynthetic bacteria biocontrol bacterium agent, adopts the capsulated rhodobacter A18 of embodiment 1 as raw material, prepares through activation, seed cultivation, production cultivation, and its specific preparation method comprises the following steps:

[0055] A kind of photosynthetic bacteria biocontrol bacterium agent, adopts the capsulated rhodobacter A18 bacterial strain of embodiment 1 as raw material, obtains through activation, seed cultivation, production cultivation, and its specific preparation method comprises the following steps:

[0056] (1) Activation: the preserved species of Rhodobacter capsularis A18 bacterial strain is cultivated by the double-layer plate culture method, and cultivated until red single colonies appear; Glucose 1g / L, YE2.5g / L, (NH 4 ) 2 SO 4 1.8g / L, Na 2 CO 3 1.0g / L, K 2 HPO 4 0.4g / L, MgSO 4 0.5g / L, agar powder 18g / L, pH=7.0.

[0057] (2) Seed culture: Put the maroon single colony cultivated in step (1) in...

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Abstract

The invention discloses a photosynthetic bacterial strain, a biocontrol bacterial agent, a biocontrol fermentation liquid, a preparation method and an application. The photosynthetic bacteria strain is Rhodobacter capsulata A18. (1) The production process of the photosynthetic bacterium Rhodobacter capsula A18 of the present invention is simple, the culture time is short, and the cost is low, and the bacterial strain can be prepared by a simple, fast, and low-cost operation method to obtain the biocontrol method for the prevention and control of citrus canker. Bacteria and biological control fermentation liquid. (2) The biocontrol agent and the biocontrol fermented liquid prepared by the photosynthetic bacterium Capsule Rhodobacter A18 of the present invention have the characteristics of environmental friendliness, nontoxicity to humans and animals, no phytotoxicity to crops, simple and convenient application, and are not easy to cause damage by diseases and insect pests. develop drug resistance.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a photosynthetic bacterial strain, a biocontrol agent, a biocontrol fermentation liquid, a preparation method and an application for preventing and treating citrus canker. Background technique [0002] As a fruit tree widely planted in the world, citrus has strong adaptability and is widely planted in various countries in the world. In the citrus production, due to the damage of diseases and insect pests, the citrus industry has brought huge economic losses. Among them, citrus canker is one of the most dangerous diseases of citrus, and it is also one of the most important and common diseases in citrus producing areas in my country, especially in Guangdong, Guangxi, Hunan, Hubei and Fujian provinces. Moreover, in the past ten years, the occurrence and prevalence of citrus canker has been increasing. Citrus canker, as a bacterial disease, is a citrus disease caused by Xanth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00C12R1/01
CPCA01N63/00C12R2001/01C12N1/205A01N63/10
Inventor 程菊娥苏品刘勇张德咏郑立敏王忠勇周波
Owner HUNAN PLANT PROTECTION INST
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