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Aptamer sensor and preparation method thereof

An aptamer sensor and nucleic acid aptamer technology, applied in instruments, scientific instruments, measuring devices, etc., can solve the problems of low sensitivity, complicated testing process, and poor reproducibility, and achieve high sensitivity and selectivity, high preparation efficiency, and detection low limit effect

Active Publication Date: 2019-10-15
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods usually have disadvantages such as low sensitivity, complicated testing process and poor reproducibility

Method used

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  • Aptamer sensor and preparation method thereof
  • Aptamer sensor and preparation method thereof
  • Aptamer sensor and preparation method thereof

Examples

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preparation example Construction

[0039] In the preparation method of the aptamer sensor of the present invention, the electrode material for the aptamer sensor is dispersed in ethanol to obtain an electrode material suspension for the aptamer sensor.

[0040] Preferably, the concentration of the electrode material suspension for the aptamer sensor is 1 mg / mL.

[0041] In the preparation method of the aptamer sensor of the present invention, the coating amount of the electrode material suspension for the aptamer sensor on the electrode surface is 5.0 µL.

[0042] In the preparation method of the aptamer sensor of the present invention, the drying time of the modified electrode is 3-8h. The drying temperature is room temperature. The drying temperature can be 20-30°C.

[0043] The water was Milli-Q water (the resistivity at 25°C was 18.2Ω·cm).

[0044] Reagents and materials:

[0045] Nickel nitrate (Ni(NO 3 ) 2 ·6H 2 O), K 3 [Co(CN) 6 ] Purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Sodium citrat...

Embodiment 1

[0054] The preparation method of the aptamer sensor of this embodiment includes the following steps:

[0055] (1) Preparation of nickel-cobalt Prussian blue analogs

[0056] At room temperature, 0.6 mM (174 mg) of nickel nitrate and 0.6 mM (265 mg) of sodium citrate were dissolved in 20 mL of deionized water to form solution A.

[0057] At room temperature, potassium (III) hexacyanocobaltate (134 mg) was dissolved in 20 mL of deionized water to form solution B.

[0058] At 37°C, while stirring, add solution B to solution A dropwise until a precipitate is formed. The precipitate is separated. The precipitate is washed three times with ethanol, and then dried in a vacuum oven at 60°C for 10 hours to obtain a nickel cobalt Prussian blue analog , Marked as NiCo PBA.

[0059] (2) Pretreatment of bare gold electrode

[0060] First, polish the bare gold electrode with 0.05mm alumina slurry and rinse with ultrapure water.

[0061] Then, in the piranha solution (v / v, H 2 SO 4 : H 2 O 2 = 7: 3) Ri...

Embodiment 2- Embodiment 6

[0070] Example 2-The preparation method of the aptamer sensor of Example 6. The difference from Example 1 is that the concentration of NiCo PBA in step (3) is different, and the rest of the steps are the same as Example 1, Example 1 and Example 2. -The concentration of NiCo PBA in Example 6 is shown in Table 1.

[0071] Table 1 Concentration of NiCo PBA in Example 1 and Example 2-Example 6

[0072]

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Abstract

The invention relates to an aptamer sensor and a preparation method thereof, and belongs to the technical field of electrochemical sensors. The invention provides the aptamer sensor comprising an electrode, an electrode material coated to the surface of the electrode and used for the aptamer sensor, and a nucleic acid aptamer attached to the electrode material used for the aptamer sensor, whereinthe electrode material used for the aptamer sensor is nickel-cobalt Prussian-blue analogue. The aptamer sensor exhibits good selectivity, stability, reproducibility, reactivity and applicability and low limit of detection. For example, the aptamer sensor obtained by a bare gold electrode, nickel-cobalt Prussian-blue analogue and carcino-embryonic antigen nucleic acid aptamer exhibits high sensitivity and selectivity on CEA (Earcino-Embryonic Antigen) detection in a range of 1.0fg.mL<-1>-5.0ng.mL<-1> (1.62fM), and the application of the nickel-cobalt Prussian-blue analogue is widened.

Description

Technical field [0001] The invention relates to an aptamer sensor and a preparation method thereof, and belongs to the technical field of electrochemical sensors. Background technique [0002] Carcinoembryonic antigen (CEA) is considered a tumor marker. Since it was first confirmed to be a tumor-associated antigen in 1965, it has played an important role in the diagnosis and screening of malignant tumors, including lung cancer, breast cancer, and gastrointestinal cancer. And ovarian cancer, especially clinical studies have shown that elevated CEA levels indicate tumor recurrence, so it plays a key role in colorectal cancer surgery. Therefore, it is particularly important to develop accurate quantitative methods for the determination of CEA. Various methods have been developed to detect CEA, including colorimetric immunoassay, electrochemical immunoassay, electrochemiluminescence immunoassay, fluorescence immunoassay, and chemiluminescence immunoassay. However, these methods usu...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/48C01C3/08
CPCC01C3/08G01N27/308G01N27/3275G01N27/3277G01N27/3278G01N27/48
Inventor 杜淼何领好贾巧娟李真真张治红王明花宋英攀
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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