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Seedling-stage identification method for zucchini root rot resistance

A technology of anti-root rot and identification method, which is applied in the direction of using spores, methods based on microorganisms, gardening methods, etc., can solve the problems of not being able to reflect the resistance level of root rot of gourd rootstocks, and achieve accurate and reliable identification results. Improved screening efficiency and simple operation

Active Publication Date: 2019-10-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the specialized identification of pathogen and host, the screening results of Fusarium solani can not reflect the root rot resistance level of cucurbit rootstock

Method used

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  • Seedling-stage identification method for zucchini root rot resistance
  • Seedling-stage identification method for zucchini root rot resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Isolation and identification of cucurbit root rot pathogen:

[0023] Collect diseased plants with typical root rot symptoms (the aboveground part of the plant wilts and dies, the roots and hypocotyls rot, leaving only vascular bundles), take the roots and hypocotyl tissues of the diseased plants, wash them with running water for 5 minutes, absorb the water with filter paper, and transfer In the aseptic operating table, sterilize with 70% absolute ethanol for 30 seconds, 10% sodium hypochlorite for 4 minutes, wash with sterile water for 3 times, place on sterile filter paper to control the moisture, inoculate in PDA medium, and incubate in the dark at 28°C for 3 to 5 days Until the hyphae grow out, the hyphae around the diseased block are picked and transferred to PDA medium, and cultivated in the dark at 28°C for 5 days. The spores were washed out with sterile water for single spore purification. After the obtained pure culture was cultured in the dark at 28°C for 5 da...

Embodiment 2

[0025] Identification of root rot resistance at seedling stage on 37 gourd rootstock germplasm resources:

[0026] (1) Prepare cucurbit root rot fungus and prepare root rot fungus spore suspension: get the cucurbit root rot fungus bacterial strain after purification and identification in Example 1 and inoculate it on the potato slant medium, store it at 4°C, and pick the root with a sterilized inoculation loop Mycelium of rot fungus, inoculated on potato solid medium containing 50ng / mL streptomycin, placed in an incubator at 25°C, cultivated in the dark for 7 days, cut 1.0×1.0cm bacterial block and inoculated it in liquid mung bean soup medium (mung bean soup) 20g, deionized water 1000mL), cultivated on a shaker at 28°C, 150-200rpm / min for 2 days, collected and diluted the spore liquid, and adjusted the concentration of the spore liquid to 1x10 6 individual / mL to obtain root rot fungus spore suspension;

[0027] (2) There are 37 parts of gourd rootstock germplasm resource mat...

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Abstract

The invention relates to a seedling-stage identification method for zucchini root rot resistance, and belongs to the technical field of crop disease resistance identification. The method comprises thefollowing steps of: firstly, preparing a root rot fungus spore suspension by taking zucchini root rot fungus, wherein the concentration is 10<6> / mL; after zucchini seedling cotyledons are flattened,injecting the zucchini root rot fungus spore suspension at the root for inoculation; and after 20 days, investigating the disease stages of each plant, calculating the disease index, and evaluating the disease resistance of zucchini rootstock germplasm resources against the root rot fungus. The invention establishes a seedling-stage identification method for zucchini rootstock root rot resistance.The identification method is seedling-stage identification, is simple to operate, fast and efficient, has accurate and reliable identification result, can obviously distinguish the difference of theresistance of different zucchini rootstock materials to root rot, is suitable for identifying and screening root rot disease resistance of mass materials, and improves the screening efficiency of germplasm resource materials.

Description

technical field [0001] The invention relates to a method for identifying root rot resistance of gourds at the seedling stage, and belongs to the technical field of crop disease resistance identification. Background technique [0002] Grafting technology is an important means to effectively control watermelon wilt caused by Fusarium oxyxporumf.sp.niveum infection. At present, the commonly used watermelon rootstock types in production mainly include gourds, pumpkins, wax gourds and wild watermelons. However, with the continuous increase of the cultivation area of ​​grafted watermelon facilities, in recent years, in different main watermelon producing areas in my country, the root of grafted watermelon plants has been infected frequently, and the leaves withered until the plants died, resulting in a decline in watermelon production and serious economic losses. . Symptoms of the disease are similar to those of watermelon wilt, but they are different from Fusarium wilt. Its char...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12N1/14C12N3/00A01G7/00C12R1/645
CPCC12Q1/18C12Q1/04C12N1/14C12N3/00A01G7/00G01N2333/37
Inventor 张曼羊杏平徐锦华刘广姚协丰
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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