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Preparation method and application of cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging life of caenorhabditis elegans

A technology of Caenorhabditis elegans and marine polysaccharide, which can be applied in application, food science, animal feed, etc., can solve the problems of limited availability and poor stability of anthocyanins, and achieve easy operation, good biocompatibility and degradability. , easy to cultivate effect

Inactive Publication Date: 2019-10-15
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the availability of anthocyanins is limited due to their poor stability

Method used

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  • Preparation method and application of cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging life of caenorhabditis elegans
  • Preparation method and application of cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging life of caenorhabditis elegans
  • Preparation method and application of cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging life of caenorhabditis elegans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Preparation of cationic marine polysaccharide-anthocyanin polyelectrolyte complex

[0044] S1: Weigh 40 mg of cranberry anthocyanin solid powder and dissolve it in 100 mL of aqueous acetic acid solution with a volume fraction of 2% (V acetic acid / V water) to obtain solution A;

[0045] S2: Add 10 mg of chitosan solid powder and 10 mg of pectin solid powder into the solution A described in step S1, and undergo ultrasonic dispersion treatment at an ultrasonic frequency of 20 KHz for 30 minutes, and homogenate for 30 minutes at a rotational speed of 4000 rpm to obtain solution B;

[0046] S3: The solution B described in step S2 is subjected to vacuum freeze-drying at a temperature of -50° C., a vacuum pressure of 40 Pa, and drying for 24 hours to obtain a cationic marine polysaccharide-anthocyanin polyelectrolyte complex.

Embodiment 2

[0047] Example 2: Preparation of cationic marine polysaccharide-anthocyanin polyelectrolyte complex

[0048] S1: Weigh 40 mg of cranberry anthocyanin solid powder and dissolve it in 100 mL of aqueous acetic acid solution with a volume fraction of 2% (V acetic acid / V water) to obtain solution A;

[0049] S2: Add 20 mg of chitosan solid powder and 20 mg of pectin solid powder into the solution A described in step S1, and undergo ultrasonic dispersion treatment at an ultrasonic frequency of 20 KHz for 30 minutes, and homogenate for 30 minutes at a rotational speed of 4000 rpm to obtain solution B;

[0050] S3: The solution B described in step S2 is subjected to vacuum freeze-drying at a temperature of -50° C., a vacuum pressure of 40 Pa, and drying for 24 hours to obtain a cationic marine polysaccharide-anthocyanin polyelectrolyte complex.

Embodiment 3

[0051] Example 3: Preparation of cationic marine polysaccharide-anthocyanin polyelectrolyte complex

[0052] S1: Weigh 40 mg of cranberry anthocyanin solid powder and dissolve it in 100 mL of aqueous acetic acid solution with a volume fraction of 2% (V acetic acid / V water) to obtain solution A;

[0053] S2: Add 30 mg of chitosan solid powder and 30 mg of pectin solid powder into the solution A described in step S1, and undergo ultrasonic dispersion treatment at an ultrasonic frequency of 20 KHz for 30 minutes, and homogenate for 30 minutes at a rotational speed of 4000 rpm to obtain solution B;

[0054] S3: The solution B described in step S2 is subjected to vacuum freeze-drying at a temperature of -50° C., a vacuum pressure of 40 Pa, and drying for 24 hours to obtain a cationic marine polysaccharide-anthocyanin polyelectrolyte complex.

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Abstract

The invention discloses a preparation method of a cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging the life of caenorhabditis elegans. The preparation method comprises the steps that bilberry anthocyanin is taken to be dissolved into an acetic acid aqueous solution with the volume fraction being 1%-2%, and thus a solution A with the bilberry anthocyanin concentration being 0.2-2 mg / ml; a wall material is added into the solution A, and ultrasonic dispersion and slurry homogenizing are conducted to obtain a solution B; the wall material is prepared from chitosan and pectin according to the weight ratio of (1:1) to (1:3); the weight ratio of the bilberry anthocyanin to the wall material in the solution A is (2:1) to (2:4); and the solution B is subjected to vacuum freeze-drying, and thus the cationic marine polysaccharide-anthocyanin polyelectrolyte compound is obtained. Through the prepared cationic marine polysaccharide-anthocyanin polyelectrolyte compound, the life of the caenorhabditis elegans can be prolonged, and the reproductive capacity can be enhanced; and the marine polysaccharide-anthocyanin polyelectrolyte compound can be used for preparation of food and health care products, drug loading, drug slow release and the like.

Description

technical field [0001] The invention belongs to the technical fields of food, health products and animal models, and in particular relates to a preparation method and application of a cationic marine polysaccharide-anthocyanin polyelectrolyte complex capable of prolonging the lifespan of Caenorhabditis elegans. Background technique [0002] As a model organism, Caenorhabditis elegans (C. elegans) is called a nematode for short because it is a non-toxic and harmless nematode that can live independently. Its body is small, the adults are only 1.2mm long and 0.08mm wide, and the whole body is transparent. N2 wild-type Caenorhabditis elegans is a commonly used species in the laboratory. The normal life cycle is only about 3 weeks, divided into 6 stages, embryonic stage, L1 stage, L2 stage, L3 stage, L4 stage, adult stage, L4 stage after a molt That is to enter the adult stage to lay eggs. It feeds on uracil-deficient Escherichia coli (E.coli) OP50. It has a simple structure an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L29/30A23P10/30A01K67/033A23K50/00A23K20/121A23K20/163
CPCA23L33/105A23L29/30A23P10/30A01K67/033A23K50/00A23K20/121A23K20/163
Inventor 谭明乾赵雪张雪迪铁珊珊刘赫非
Owner DALIAN POLYTECHNIC UNIVERSITY
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