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A kind of method utilizing sodium chloride to regulate the synthetic stability of polyhydroxyalkanoate of mixed flora

A technology of polyhydroxyalkanoate and mixed flora, which is applied in the direction of using microorganisms, fermentation, etc., and can solve the problems of high input cost, long adjustment period, and poor operability

Active Publication Date: 2020-12-11
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the traditional recovery techniques and theories are based on the activated sludge method. By adjusting process parameters and optimizing environmental factors, the adjustment period is long, the operability is poor, and the input cost is high.

Method used

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  • A kind of method utilizing sodium chloride to regulate the synthetic stability of polyhydroxyalkanoate of mixed flora
  • A kind of method utilizing sodium chloride to regulate the synthetic stability of polyhydroxyalkanoate of mixed flora

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specific Embodiment approach 1

[0023] Specific embodiment one: the sodium chloride of the present embodiment is used for the method for rapid recovery destabilizing polyhydroxyalkanoate mixed flora production process, and concrete steps are as follows:

[0024] 1. Add 3-5g / L sodium chloride to the domesticated SBR reactor of PHA mixed flora caused by the expansion of filamentous bacteria to produce sludge loss. The volume load of the SBR reactor is 1000-2000mgCOD / L / d, The substrate carbon source is a combination of acetic acid, propionic acid, butyric acid, valeric acid and ethanol, the SRT is 10d, the HRT is 1d, and the operation cycle is 12h;

[0025] 2. Take 100mL of the mixed solution at the end of the period from the SBR reactor, and observe the daily reactor sludge sedimentation ratio (SV 30 ) and mixed liquor suspended solids concentration (MLSS), calculate the sludge volume index (SVI);

[0026] Three, such as SV 30 Not higher than 50%, SVI not greater than 200, MLSS value not lower than 2500mg / L,...

specific Embodiment approach 2

[0033] Embodiment 2: This embodiment is different from Embodiment 1 in that: the SBR reactor is under the condition of 15-25° C., and the aeration is continued. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the operation period of the SBR reactor is 6-12 hours. Others are the same as in the first embodiment.

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Abstract

The invention provides a method for controlling synthesis stability of polyhydroxyalkanoate of the mixed flora through sodium chloride and relates to a method for restoring stability of a production technology for the mixed flora with polyhydroxyalkanoate. The method for controlling the synthesis stability comprises the steps that sodium chloride is added to a PHA synthesis bacterium gathering reactor where sludge bulking occurs, the sludge settling ratio SV30 and MLSS of the reactor are detected, and the sludge volume index SVI is calculated; if the SV30 is lower than 50%, the SVI is lower than 200, and the duration time exceeds one SRT, normal salt-free operation can be restored; if bulking is not obviously improved, the addition amount of the sodium chloride continues to be increased until all indexes reach the demand, and normal operation is restored. According to the method, the situation can be quickly relieved in short time that due to filamentous fungus swelling, the domestication reactor is unstable, and even the severe sludge loss is caused. The situation is prevented in time that due to an unstable technology, the biomass and PHA yield are reduced. The operation loss isreduced.

Description

technical field [0001] The invention belongs to the field of synthesis of biodegradable plastics and recovery of waste resources, and relates to a method for recovering and stabilizing the production process of polyhydroxyalkanoate (PHA) mixed flora, specifically using sodium chloride to quickly restore the unstable polyhydroxyalkanoate mixture The flora production process is a process method that is low-cost and quickly inhibits sludge loss caused by sludge bulking and can still maintain PHA production during the recovery stage, reducing losses caused by system instability. Background technique [0002] Traditional industrialization and modernization have given birth to polymer materials represented by petrochemical plastics. Although their large-scale application has brought great convenience to human society, it has also brought serious damage to the natural environment. Traditional plastic products that are difficult to biodegrade that have been produced and used by huma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C12N1/36
CPCC12N1/36C12P7/625
Inventor 温沁雪季业陈志强
Owner HARBIN INST OF TECH
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