Bacillus amyloliquefaciens with inhibiting effect on fusarium oxysporum and application of bacillus amyloliquefaciens
A kind of technology of amylolytic spores and watermelon specialization, applied in the field of agricultural biology, can solve the problems of labor and time consumption, low genetic diversity of watermelon, and destruction of ecological balance, etc., and achieve significant antibacterial effect, great development and application value Effect
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[0036] Example 1 Screening and identification of Bacillus amyloliquefaciens LZN01 and its application in inhibiting Fusarium oxysporum (FON)
[0037] 1Materials and methods
[0038] 1.1 Test materials
[0039] Test drug: phosphate buffer (pH 7.2): prepare Na 2 HPO 4 And NaH 2 PO 4 Stock solution, weigh Na 2 HPO 4 138g and NaH 2 PO 4 142g, respectively dissolve in appropriate amount of water, and dilute to 1L after dissolving; measure 72mL Na 2 HPO 4 Solution and 28mLNaH 2 PO 4 Solution, after mixing, obtain a phosphate buffer solution with pH 7.2, store in a refrigerator at 4℃; 2.5% glutaraldehyde buffer solution: 0.2mol / L phosphate buffer 50mL plus 25% glutaraldehyde solution 10mL, double distilled water to make the volume to 100mL .
[0040] Test medium: PDA medium (g / L): potato 200g, glucose 20g, distilled water 1000mL, agar 15-20g, sterilized at 120℃ for 20min; LB medium (g / L): peptone 10g, yeast extract 5g, NaCl 8g, distilled water 1000mL, 120℃ sterilized for 20min.
[0041] Te...
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