Ralstonia pickettii M1 strain and application thereof in degrading phenanthrene and biphenyl

A strain and biphenyl technology, applied in the field of microorganisms, can solve problems such as environmental pollution and chlorinated biphenyl leakage, and achieve the effect of good application potential

Active Publication Date: 2019-10-11
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Halogenated biphenyl derivatives, especially polychlorinated biphenyls, are widely produced at home and abroad as the main components of industrial raw materials. During the early application of chlorinated biphenyls, a large amount of chlorinated biphenyls leaked into the environment, causing environmental pollution. long-term pollution

Method used

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  • Ralstonia pickettii M1 strain and application thereof in degrading phenanthrene and biphenyl
  • Ralstonia pickettii M1 strain and application thereof in degrading phenanthrene and biphenyl
  • Ralstonia pickettii M1 strain and application thereof in degrading phenanthrene and biphenyl

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Embodiment 1

[0018] The domestication, screening and isolation of embodiment 1 Ralstonia pickettii (Ralstonia pickettii) M1 bacterial strain

[0019] 1. Sample source

[0020] Sewage samples were collected from the sewage of an electronic waste recycling plant in Qingyuan, Guangdong, and high-concentration phenanthrene and biphenyl were used as carbon sources for long-term domestication, and efficient phenanthrene and biphenyl-degrading bacteria were obtained through multiple screening and separation and purification.

[0021] 2. Medium

[0022] (1) Inorganic salt medium: Inorganic salt medium is used for the enrichment culture of microorganisms in the sample and the degradation experiment of phenanthrene and biphenyl under the condition of pure bacteria. The medium formula is shown in Table 1 (containing phenanthrene or biphenyl solution).

[0023] Table 1 Inorganic salt medium formula

[0024]

[0025] (2) Nutrient medium: Nutrient medium is used for the cultivation of conventional...

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Abstract

The invention discloses a Ralstonia pickettii M1 strain and application thereof in degrading phenanthrene and biphenyl. The M1 strain is a degraded strain obtained through acclimation and separation of sewage from a certain electronic waste recycling plant in Qingyuan, Guangdong Province by taking the phenanthrene and the biphenyl as carbon sources. According to the morphology, physiological characteristics, Gram reaction, 16S rDNA gene sequencing analysis and phylogenetic analysis of the strain, the strain is identified as Ralstonia pickettii M1. The strain can utilize the phenanthrene and the biphenyl as the carbon sources, and the degradation rate can reach 60% or above after culture is performed for 3 days in an inorganic salt culture solution with an initial concentration of 100 mg / Lof the phenanthrene and biphenyl. Therefore, the Ralstoniapickettii M1 strain has a better application potential in the bioremediation of polycyclic aromatic hydrocarbon and polychlorinated biphenyl.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to Ralstonia pickettii M1 strain and its application in degrading phenanthrene and / or biphenyl. Background technique [0002] With the rapid development of modern industrial processes, the pollution of industrial wastewater is becoming more and more serious. Various persistent organic pollutants (POPs), such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), exist in industrial wastewater. PAHs are ubiquitous and accumulating in the environment, and have received widespread attention. Due to factors such as industrial mining, agriculture and other human activities and high background value of soil environment, PAHs in the environment have been seriously polluted and seriously exceeded, and the main pollutants in chemical parks and surrounding soils, oil production areas, mining areas, and sewage irrigation areas are all is caused by PAHs. Phen...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/30
CPCB09C1/10C02F3/34C02F2101/30C12N1/205C12R2001/01
Inventor 李继兵罗春玲李军张干
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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