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Preparation and application method of double network hydrogel for tumor microsphere invasion detection

An intrusion detection, hydrogel technology, applied in material analysis using radiation diffraction, measuring devices, material analysis using radiation, etc., to achieve the effect of reducing variance

Active Publication Date: 2020-11-20
江苏艾玮得生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on tumor microsphere invasion detection

Method used

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  • Preparation and application method of double network hydrogel for tumor microsphere invasion detection
  • Preparation and application method of double network hydrogel for tumor microsphere invasion detection
  • Preparation and application method of double network hydrogel for tumor microsphere invasion detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Preparation and application of alginate / methacrylic acid modified gelatin (Alginate / Gel-MA) double network hydrogel

[0052] Dissolve the gelatin in a sodium carbonate-sodium bicarbonate buffer solution with a pH of 9.5 and a concentration of 0.25 mol / L, and stir evenly at 60°C. The gelatin concentration is 10% (W / V), methacrylic anhydride is passed into the dissolved gelatin, the ratio of methacrylic anhydride to gelatin is 0.58mL / 10g, and the reaction is carried out at 50°C for 2 hours. After the reaction, dialysis was performed at 40° C. for 7 days, and the dialysate was freeze-dried at -80° C. to obtain methacrylic acid-modified gelatin with a molar substitution degree of 0.6.

[0053] Take 1 g of the above-mentioned methacrylic acid-modified gelatin and dissolve it in 0.01 M sodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, so that the percentage concentration of the methacrylic acid-modified gelatin is 20% (W / V). Dissolve lithium phenyl (2,4,...

example 2

[0059] Preparation and application of type I collagen / methacrylic acid modified gelatin (Collagen / Gel-MA) double network hydrogel

[0060] Dissolve the gelatin in a sodium carbonate-sodium bicarbonate buffer solution with a pH of 9.5, and stir evenly at 60°C. The concentration of gelatin is 10% (W / V), methacrylic anhydride is passed into the dissolved gelatin, the ratio of methacrylic anhydride and gelatin is 0.4mL / 10g, and the reaction is carried out at 50°C for 2 hours. After the reaction, dialysis was performed at 40°C for 7 days, and the dialysate was freeze-dried at -80°C to obtain methacrylic acid-modified gelatin with a molar substitution degree of 0.35.

[0061] Take 1 g of the methacrylic acid-modified gelatin and dissolve it in 0.01 M sodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, so that the concentration of the methacrylic acid-modified gelatin is 20% (W / V).

[0062] Dissolve lithium phenyl (2,4,6-trimethylbenzoyl) phosphate in ultrapure wa...

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Abstract

The invention provides a preparation method and an application method of bioactive specific double-network hydrogel for 3D tumor intrusion detection. Self-prepared methyl propylene modified gelatin iscompounded with natural macromolecules and synthetic macromolecules to obtain the double-network hydrogel. Through proportion regulation of the two materials, and through substitution degree and concentration regulation of the methyl propylene modified gelatin, the double-network hydrogel has multiple mechanical properties and can conform to a variety of real human tissue environments in a targeted way. The double-network hydrogel can be used for tumor microsphere intrusion detection. The two different mechanical properties of the double-network hydrogel, biocompatibility and tumor microsphere intrusion detection are tested, represented and illustrated through two embodiments, proving that test results are effective.

Description

technical field [0001] The invention relates to a method for preparing a specific double network hydrogel, which is used for detecting the 3D invasion ability of tumors, belongs to the technical fields of cell biology and biomedical material preparation, and in particular relates to the field of composite preparation of biological materials. Background technique [0002] Tumor is currently the most important factor threatening human health. 90% of the mortality rate of clinical tumor patients is caused by tumor invasion into other organs and tissues. The process of tumor invasion is complex and difficult to control. To some extent, preventing tumor invasion can control the death caused by tumor. Therefore, tumor invasion detection becomes particularly important. In vitro three-dimensional culture is particularly suitable for simulating the tumor microenvironment, and can better simulate the biological characteristics of tumors. It is very necessary to construct a suitable ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24C08J3/28C08L89/00C08L5/04G01N23/04G01N23/20G01N23/20008
CPCC08J3/075C08J3/246C08J2389/00C08J2405/04G01N23/2251
Inventor 顾忠泽陈早早王菲张静孙晓玮葛健军
Owner 江苏艾玮得生物科技有限公司
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