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Method for measuring contents of chlorogenic acid, caffeic acid and galuteolin in ixeris chinensis by quantitative assay of multi-components by single-marker

A technology of luteolin and chlorogenic acid, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of high practicability, simple method, comprehensive and effective evaluation and control

Active Publication Date: 2019-10-01
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few relevant researches on the quality analysis of chrysanthemum, after reviewing the literature, there is no report on the determination of chlorogenic acid, caffeic acid and luteolin in chrysanthemum at home and abroad.

Method used

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  • Method for measuring contents of chlorogenic acid, caffeic acid and galuteolin in ixeris chinensis by quantitative assay of multi-components by single-marker
  • Method for measuring contents of chlorogenic acid, caffeic acid and galuteolin in ixeris chinensis by quantitative assay of multi-components by single-marker
  • Method for measuring contents of chlorogenic acid, caffeic acid and galuteolin in ixeris chinensis by quantitative assay of multi-components by single-marker

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Experimental program
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Effect test

Embodiment 1

[0024] Using the above-mentioned method of one test and multiple evaluations to determine the content of chlorogenic acid, caffeic acid and luteolin in C. chinensis, 10 batches of C. cicadae were tested. The specific determinations are as follows:

[0025] 1 Experimental materials, instruments and reagents

[0026] 1.1 Experimental materials

[0027] See Table 2 for the harvesting area and date of the Chinese herbal medicines, and it was identified by Professor Wei Songji of Guangxi University of Traditional Chinese Medicine as a plant of the genus A. Ixeridium chinense (Thunb.) Tzvel.) Whole plant.

[0028]

[0029] 1.2 Instruments

[0030] U3000 high-performance liquid chromatography (Thermo Fisher, USA); 1260 high-performance liquid chromatography (Agilent, USA); column CAPCELLPAK TYPEⅡSIZE C18 (4.6mmI.D.×250mm, 5µm), AgilentEclipse plus C18 (4.6×250mm, 5µm), Waters Atlantis T3 (4.6×250mm, 5µm); XS-205Du electronic analytical balance (Switzerland METTLER TOLEDO company)...

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Abstract

The invention relates to the technical field of Chinese herbal medicinal ingredient detection, in particular to a method for measuring contents of chlorogenic acid, caffeic acid and galuteolin in ixeris chinensis by quantitative assay of multi-components by single-marker. The method comprises the following steps of (1) preparing a reference solution; (2) preparing a test solution; (3) performing chromatographic condition and system applicability experiment; and (4) performing measurement. The chlorogenic acid is used as an internal reference substance for multi-components by single-marker, a relative correction factor among the chlorogenic acid, the caffeic acid and the galuteolin is built, and the contents of the chlorogenic acid, the caffeic acid and the galuteolin in the ixeris chinensis are calculated by the relative correction factor. The method is simple, is convenient to operate and is high in practicability and accurate in result; and by the method, synchronous measurement of the contents of the chlorogenic acid, the caffeic acid and the galuteolin in the ixeris chinensis can be achieved, so that the quality of an ixeris chinensis medicine is effectively evaluated and controlled, and scientific basis is provided for guarantee of clinical medication safety and effectiveness and development of medicinal resources.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicine components, in particular to a method for measuring the contents of chlorogenic acid, caffeic acid and luteolin in chrysanthemum by adopting a one-test and multiple-evaluation method. Background technique [0002] Mountain bittern ( Ixeridium chinense (Thunb.) Tzvel.) is a perennial herbaceous plant of the family Asteraceae. The whole herb is used as medicine. It is also known as Chinese bitter vegetable, mountain bitter vegetable, small bitter vegetable, etc. It is distributed in northern, southern and eastern provinces and regions. It can be used as medicine It is edible and edible. It has the effects of clearing heat and detoxifying, reducing inflammation and cooling blood, relieving pain and swelling, and anti-tumor. It is used to treat unknown swelling and pain, abdominal abscess, dysentery, appendicitis, pneumonia, arthritis and other diseases. [0003] Liu...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8679G01N30/88G01N2030/8809
Inventor 梁爽朱华赵立春黄健军卢森华陈洁银吴秀彩
Owner GUANGXI UNIV OF CHINESE MEDICINE
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