Method for bioremediation of crude oil polluted soil by utilizing plants and cryogel
A contaminated soil and bioremediation technology, applied in the restoration of contaminated soil, etc., can solve the problems of low degradation and removal rate of crude oil pollutants, unsatisfactory effect, and certain difficulties, and achieve industrial scale-up and easy application, reducing the repair process Cost, effect of improving survivability
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Embodiment 1
[0026] Put 1 kg of CSETIA-BR series starch compound crystal gel microbial restoration agent for immobilizing Rhodococcus and yeast into 1.25m 3 In the crude oil polluted soil, mix evenly in situ, and the oil content of the polluted soil is 4.8% (w / w, the same below) as detected by gravimetric method; add the compound fertilizer containing N, P, K, etc. required for plant growth and the microbial The required nutrients such as carbon source and nitrogen source substrate are 0.1 kg, and then flattened to form a mixed soil with a thickness of 20-30 cm; sow the seeds of Carnation double-petal in the mixed soil, supplement water to maintain the seeds germination; maintain suitable light and ventilation , moisturizing and other degradation conditions, the natural temperature is about 20~35 o C. Maintain plant growth and microbial metabolism; after one month of restoration, through the synergistic effect of plant growth and microbial degradation, the oil content of the polluted soil ...
Embodiment 2
[0028] Put 6 kg of CSETIA-BR series starch compound crystal gel microbial restoration agent for immobilizing Rhodococcus and yeast into 3 m 3 In the crude oil contaminated soil, mix evenly in situ, and the oil content of the contaminated soil is 3% by gravimetric method; add the compound fertilizer containing N, P, K, etc. required for plant growth and the carbon source and nitrogen source required for microbial growth 0.3 kg of food and other nutrients, and then flattened to make a mixed soil with a thickness of 20-30 cm; sow alfalfa seeds in the mixed soil, supplement water to maintain seed germination; maintain suitable degradation conditions such as light, ventilation, and moisture retention, and the natural temperature is about 20 ~35 o C. Maintain plant growth and microbial metabolism; after one month of restoration, through the synergistic effect of plant growth and microbial degradation, the oil content of the polluted soil was detected by gravimetric method to be 0.6%...
Embodiment 3
[0030] Put 20 kg of CSETIA-BR series starch compound crystal gel microbial restoration agent for immobilizing Rhodococcus and yeast into 2.5m 3 In the crude oil contaminated soil, mix evenly in situ, and the oil content of the contaminated soil is 0.3% by gravimetric method; add the compound fertilizer containing N, P, K, etc. required for plant growth and the carbon source and nitrogen source required for microbial growth 10 kg of food and other nutrients, and then flattened to form a mixed soil with a thickness of 20-30cm; sow the seeds of Carnation double-petal in the mixed soil, supplement water to maintain seed germination; maintain suitable degradation conditions such as light, ventilation, and moisture, and natural temperature about 20~35 o C, maintain plant growth and microbial metabolism; after 3 months of restoration, through the synergistic effect of plant growth and microbial degradation, the oil content of the polluted soil was detected by gravimetric method to be...
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