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Biological capsule for tissue repairing and establishing method thereof

A biocapsule and tissue repair technology, applied in the field of biomedical materials, can solve the problems of lack of inflammatory cell invasion and decomposition of biocapsules, and achieve good biocompatibility, prevent loss, enhance protection and mechanical strength

Inactive Publication Date: 2019-10-01
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the embodiment of the present invention provides a biocapsule for tissue repair and its construction method to solve the problem in the prior art that there is no biocapsule that can prevent the invasion and decomposition of inflammatory cells

Method used

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  • Biological capsule for tissue repairing and establishing method thereof
  • Biological capsule for tissue repairing and establishing method thereof
  • Biological capsule for tissue repairing and establishing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of porous nanoscale honeycomb structure

[0034] The porous nanoscale honeycomb structure is synthesized by free radical emulsion polymerization multipolymer, specifically, the preparation process is: 9.9mmol isopropylacrylamide NIPAM, 0.1mmol acrylic acid AA, 0.2mmol 2-mercaptobenzoic acid MBA and 0.2mmol Sodium dodecyl sulfate SDS was dissolved in 97 ml of water. After thorough mixing, the mixed solution was transferred to a 250ml three-necked flask equipped with a condenser and a mechanical stirrer.

[0035] The mixed solution was degassed under nitrogen for 30 min before polymerization. After degassing, the three-necked flask was placed in a preheated oil bath with a temperature of 70 °C. 3.0 ml of an aqueous KPS solution containing 0.1 mmol of potassium persulfate was injected into the mixture solution to initiate polymerization. Under the protection of nitrogen environment, the mixture solution was stirred continuously for 5 hours ...

Embodiment 2

[0036] Embodiment 2, porous nanoscale honeycomb structure

[0037] A scanning electron microscope (SEM) was used to observe the cross-section of the porous nano-scale honeycomb structure in the vertical and horizontal directions. Such as figure 2 As shown, the porous nano-scale honeycomb structure has a nano-scale pore size and can support or accommodate repair cells. The repair cells are cardiac stromal cells, mesenchymal stem cells or a combination thereof. Sources of repair cells include: tissue cells from the heart, mesenchymal stem cells from bone marrow and peripheral blood, etc. The porous nano-scale honeycomb structure is loaded with repair cells to form a biocapsule, which can better prevent the invasion of inflammatory cells, allow cells to grow better, and at the same time realize the release of small molecule bioactive substances.

Embodiment 3

[0038] Example 3, detection of internal morphology of biocapsules loaded with repaired cells

[0039] The repair cells and cardiac stromal cells were collected in the culture medium and mixed with 10XPBS and the liquid of the porous nanoscale honeycomb structure at a ratio of 1:1:3, and then heated in a 37°C incubator to gel to form biological capsule.

[0040] Observe the biocapsules with a scanning electron microscope. Specifically, the biocapsules are dehydrated and fixed, encapsulated on an aluminum post with double-sided tape and coated with a thin layer of gold. Under an accelerating voltage of 15kV, the biocapsules are scanned and photographed by a scanning electron microscope. Such as image 3 As shown, the results of the biocapsules indicated that the porous nanoscale honeycomb structure encapsulated the repair cells.

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Abstract

The embodiment of the invention discloses a biological capsule for tissue repairing and an establishing method thereof. The biological capsule comprises a porous nanoscale honeycomb-shaped structuralbody and repairing cells loaded on the porous nanoscale honeycomb-shaped structural body, wherein the porous structural body is prepared from N-isopropylacrylamide, acrylic acid, 2-mercaptobenzoic acid, lauryl sodium sulfate and water through polymerization reaction by means of the method of taking N-isopropylacrylamide, acrylic acid, 2-mercaptobenzoic acid, lauryl sodium sulfate and water to be sufficiently mixed to form mixed liquid. In the materials for preparing the biological capsule, simple and easy-to-obtain synthesis polymers with small batch-to-batch differences serve as the main rawmaterial, no immunogenicity problem exists, and good biocompatibility is realized. The biological capsule is formed after repairing cells are inoculated into the temperature-sensitive controllable porous nanoscale honeycomb-shaped structural body.

Description

technical field [0001] Embodiments of the present invention relate to biomedical materials, in particular, to a biocapsule for heart repair and a construction method thereof. Background technique [0002] Myocardial infarction is one of the most common and serious vascular diseases in coronary heart disease. The prevalence rate is increasing year by year, and it can gradually develop into chronic heart failure. The resulting medical expenses are increasing year by year, and the social and family burdens are serious. Although traditional drug therapy, interventional therapy or surgical bypass surgery and device-assisted devices used in advanced heart failure can reestablish blood supply, reduce further scarring and adverse ventricular remodeling, and increase the function of undamaged myocardium, they cannot Rescuing dead heart muscle cells fails to convert scar tissue into functional, related cells and reverses the accompanying heart failure. Heart transplantation is an ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/16A61L27/54A61L27/56
CPCA61L27/16A61L27/3834A61L27/54A61L27/56A61L2300/414A61L2300/624
Inventor 唐俊楠
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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