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Cryoprotectant solution for mesenchymal stem cells and preparation method thereof

A technology for cryoprotective solution and quality stem cells, which is applied in the field of stem cell cryopreservation and can solve problems such as cytotoxicity and adverse effects on cell growth.

Active Publication Date: 2021-08-24
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But DMSO is easy to cause serious toxicity to cells; the research results show that when the DMSO concentration in the culture medium is 10%, the cell growth inhibition rate is nearly 100%; the inhibition rate is 35% when the ‰ concentration is 0.04‰ Cell growth can also be adversely affected

Method used

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  • Cryoprotectant solution for mesenchymal stem cells and preparation method thereof
  • Cryoprotectant solution for mesenchymal stem cells and preparation method thereof
  • Cryoprotectant solution for mesenchymal stem cells and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0038]

Embodiment 2

[0040]

[0041] Wherein, the succinylated gelatin microspheres consist of the following components in parts by weight: 0.15 parts by weight of succinic anhydride; 2.5 parts by gelatin; and 1.5 parts by weight.

[0042] Described preparation method comprises:

[0043] 1) Preparation of succinylated gelatin microspheres

[0044] adding water to dissolve the gelatin by weight, adding an appropriate amount of sodium chloride to the dissolved gelatin solution to adjust the pH value to 8-10; adding the succinic anhydride to acylate the gelatin to obtain succinylated gelatin solution;

[0045] The cichoric acid of described weight part is dissolved in succinylated gelatin solution, and is dissolved in the liquid paraffin of 4% w / v mixed emulsifier, stirs evenly, with the rotating speed that is 200r / min rotating speed and stirring 25min, forms emulsion; Stir with propanol, filter with suction to obtain a filter cake; add 5 times the volume of the filter cake to an isopropanol sol...

Embodiment 3

[0050]

[0051] Wherein, the succinylated gelatin microspheres consist of the following components in parts by weight: 1.34 succinic anhydride; 4.79 gelatin; 2.24 cichoric acid.

[0052] Described preparation method comprises:

[0053] 1) Preparation of succinylated gelatin microspheres

[0054] adding water to dissolve the gelatin by weight, adding an appropriate amount of sodium chloride to the dissolved gelatin solution to adjust the pH value to 8-10; adding the succinic anhydride to acylate the gelatin to obtain succinylated gelatin solution;

[0055] Dissolve the cichoric acid in said parts by weight in succinylated gelatin solution, and dissolve in 4% w / v liquid paraffin mixed with emulsifier, stir evenly, and stir at a speed of 350r / min for 25min to form an emulsion; Stir with propanol, filter with suction to obtain a filter cake; add 5 times the volume of the filter cake to an isopropanol solution containing a crosslinking curing agent to solidify, filter with suc...

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Abstract

The invention provides a cryoprotective solution for mesenchymal stem cells, the cryoprotective solution mainly consists of the following components in parts by weight: 60-80 of normal saline; 2.2-5.87 of succinylated gelatin microspheres; L-group Amino acid hydrochloride 1.40‑3.11; Mannitol 0.3‑0.8. The cryoprotectant solution can protect mesenchymal stem cells for a long time and maintain cell viability.

Description

technical field [0001] The invention belongs to the technical field of stem cell cryopreservation, and in particular relates to a cryoprotective solution for mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are a kind of stem cells derived from mesoderm with multi-lineage differentiation ability, which widely exist in various tissues of the body, can be cultured and expanded in vitro, and can differentiate into cardiomyocytes, nerve cells and fat under specific conditions cells etc. With the in-depth research on the biological characteristics and functions of mesenchymal stem cells by scholars at home and abroad, mesenchymal stem cells can be isolated from various tissues. [0003] In the prior art, physiological saline or cell culture medium is usually used as the solution medium for clinical application of mesenchymal stem cells. If the human mesenchymal stem cells are isolated, cultured, and expanded, and imme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 董明珠李霞云潘新刘世红卢家堃
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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