Method for detecting antibacterial performance of antibacterial plastics by ATP bioluminescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting antibacterial performance of antibacterial plastics. The method comprises detection steps as follows: model selection of equipment and preparation of a reagent and a culture medium; collection and activation of strains and preparation of a bacterial suspension; construction of an ATP concentration log value lgCA-relative fluorescence intensity log value igIA standard curve and viable bacteria ATP concentration CA calibration of an inoculation bacterial solution; sample inoculation, culture and elution recovery; IA determination of a recovery solution and calculation of viable bacteria ATP concentration CA and TA of the recovery solution; calculation of an antibacterial rate R or antibacterial activity value A; and result evaluation. The method is characterized by applying an ATP fluorophotometer to precisely and quantitatively test the antibacterial performance of plastics represented by R or A. The method elutes and recovers control samples and antibacterial samples after 0h contact and 24 h culture, determines IA of the recovery solution and represents and calculates R or A by lgIA and provides result evaluation basis. The researched and developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the antibacterial performance of antibacterial plastics will support product quality improvement and market order regulation by the advanced detection technology.

Description

technical field [0001] The invention relates to an antibacterial performance testing method of antibacterial plastics, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antibacterial performance of antibacterial plastics characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of plastic antibacterial function detection. Background technique [0002] Plastic products are widely used in the fields of industry and people's livelihood. Because the additives used in the processing process provide the necessary nutrient source for the growth of bacteria, molds and other microorganisms, the sterility of plastics and their surfaces is very important for reducing the spread of diseases and improving people's health. level is very important. Since the 1990s, developed countries represented by Japan and the United States have ...

AI Technical Summary

Problems solved by technology

However, the existing detection methods have the following common problems in technical content and practical application: first, the number of experimental bacteria involved is too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; second, the experimental process is cumbersome and related operations Affected by the professional experience of the experimenter, the test error is large and lacks comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic cost are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of Textile products (textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content after sample inoculation is a fluorometric method to characterize the anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacteria / mold growth value > 0. In the recovery mode of live bacteria, test Key technical aspects such as effective conditions are not applicable to materials such as plastics and glass with non-absorbent hard surfaces and control bacterial growth values ​​< 0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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