Method for detecting antibacterial performance of antibacterial stainless steel with ATP (adenosine triphosphate) bioluminescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting antibacterial performance of antibacterial stainless steel. The method comprises detection steps as follows: sample preparation and pretreatment; selection of equipment type and preparation of reagents and culture media; strain preservation and activation and preparation of a bacterial suspension; establishment of an ATP (adenosine triphosphate) concentration logarithm value lgCA-relative fluorescence intensity logarithm value lgIA standard curve and calibration of viable bacteria ATP concentration CA of an inoculant liquid; inoculation, culture and eluting recovery of samples; IA determination of a recovery liquid and calculation of viable bacteria ATP concentration CA and TA of the recovery liquid; calculation of antibacterial ratio R or antibacterial activity value A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative test of the antibacterial performance of R or A characterized antibacterial stainless steel. According to the method, a control sample and an antibacterial sample after 0 h contact and 24 h culture are eluted and recovered, and IA of the recovery liquid is measured and R or A is characterized by lgIA and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the antibacterial performance of antibacterial stainless steel, product quality improvement is supported with a scientific and advanced detection technology.

Description

technical field [0001] The invention relates to an antibacterial performance test method of antibacterial stainless steel, specifically an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antibacterial performance of antibacterial stainless steel characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of stainless steel antibacterial function detection. Background technique [0002] Stainless steel is closely related to human life, and is widely used in many aspects such as industry and people's livelihood; in recent years, driven by technological progress and consumer demand, coated antibacterial stainless steel, surface modified antibacterial stainless steel, Composite antibacterial stainless steel and alloy antibacterial stainless steel came into being. According to the forecast of authoritative organizations, more than 70% of the ...

AI Technical Summary

Problems solved by technology

However, the existing detection methods have the following common problems in technical content and practical application: first, the number of experimental bacteria involved is too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; second, the experimental process is cumbersome and related operations Affected by the professional experience of the experimenter, the test error is large and lacks comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic cost are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 Textiles-Determination of antibacterial activity of textile products (textiles - Determination of antibacterial properties of antibacterial finishing textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence measurement method for anti-microbial / mold performance by ATP content change, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacterial / mold growth value > 0, in the recovery mode of viable bacteria and effective conditions of the test It is not applicable to materials such as stainless steel and glass with a non-absorbent hard surface and a bacterial growth value of the control sample < 0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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