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Trichoderma aculeatus fj069 and its application

A technology of FJ069 and Trichoderma aculeatus, which is applied in the fields of application, chemicals for biological control, and biocides, and can solve problems such as pesticide residue hazards, chemical pesticide pollution, and resistance of plant pathogens

Active Publication Date: 2021-07-27
贵州益百亿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the prevention and control of plant diseases is still mainly based on the control of chemical agents. The use of chemical agents leads to the resistance of plant pathogens, which increases the difficulty of prevention and control. Moreover, chemical pesticides will pollute and damage the environment, leading to the destruction of the biological chain. Pesticide residues are extremely harmful to the human body. big hazard

Method used

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  • Trichoderma aculeatus fj069 and its application
  • Trichoderma aculeatus fj069 and its application
  • Trichoderma aculeatus fj069 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Isolation and preservation of Trichoderma aculeatus FJ069

[0034] 1. Trichoderma aculeatus strain FJ069, collected from ginger fields in Xiamen City, Fujian Province.

[0035] Soil sample collection: Soil collection adopts the five-point diagonal sampling method. That is, select 5 equally divided points on the diagonal of the collection plot, and then use a soil picker with a depth of 25cm to drill a soil sample of about 15-20cm at each point, and then mix the 5 parts of soil evenly, and take about 50g of soil samples were packed into ziplock bags. Dispensed into 50ml plastic bottles in the laboratory and stored at 4°C for later use.

[0036] Isolation of Trichoderma: Using the dilution plate method, take 10g of soil samples from the refrigerator and put them into a triangular flask filled with 90ml of sterilized water and 5-10 glass beads with a diameter of 4mm, and then shake them on a shaker at 200r / min for 30min. Take 5ml and pour it into a triangu...

Embodiment 2

[0040] Example 2: Determination of β-1,3-glucanase enzyme activity

[0041] Preparation of DNS reagent: Weigh 91g of potassium sodium tartrate and dissolve it in 500mL of distilled water, add 3.15g of 3,5-dinitrosalicylic acid and 20g of NaOH to the solution in turn, heat and stir to dissolve, then add 2.5g of phenol , 2.5g of anhydrous sodium sulfite, stirred to dissolve it, cooled to 1000ml, and stored in a brown bottle.

[0042] Preparation of β-1,3-glucanase production medium: yeast powder 3g, NaNO 3 0.3g, MgSO 4 ·7H 2 O 0.05g, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.001g, KCl 0.05g, dilute to 100ml with distilled water, and sterilize by autoclaving at 121°C.

[0043] Preparation of glucose standard curve: Accurately weigh 1000mg of anhydrous glucose dried at 105°C to constant weight, dissolve in distilled water, and set the volume to 1000ml to obtain a 1mg / ml glucose standard solution. Take 7 graduated test tubes, add 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2ml of glucose solution...

Embodiment 3

[0049] Embodiment 3: assay of chitinase enzyme activity

[0050] Preparation of colloidal chitin: Take 10 g of chitin and add a little distilled water to grind it, then dissolve it in 375 ml of concentrated hydrochloric acid. Place in the refrigerator at 4°C for 24 hours to completely dissolve the chitin, then filter with gauze, and distill the filtrate to 1000ml with distilled water, and keep stirring. Centrifuge at 3000rpm for 10min, discard the supernatant, wash the precipitate with distilled water, dissolve and then centrifuge, repeat the operation 10-15 times until the pH value of the solution is neutral, and finally set the volume to 1000ml.

[0051] Chitin Enzyme Production Medium: Chitin 0.5g, MgSO 4 ·7H 2 O 0.06g, FeSO 4 ·7H 2 O 0.01g, NH 4 NO 3 0.3g, KH 2 PO 4 0.2g, dilute to 100ml with distilled water, and sterilize at 121°C.

[0052] Establishment of N-acetylglucose standard curve: Prepare N-acetylglucosamine (NAG) 1mg / ml standard solution, take 7 test tu...

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Abstract

What the present invention relates to is Trichoderma aculeatus FJ069 and application thereof, this strain Trichoderma aculeatus ( Trichodema asperellum ) FJ069, the preservation number of this strain is CGMCC No.17976. The Trichoderma aculeatus strain provided by the present invention has a good effect of promoting seed germination, promoting root growth of crops and preventing and controlling tomato gray mold, increases the weight of pepper roots by 117%, and has a control effect on tomato gray mold of 84.74%.

Description

[0001] 1. Technical field [0002] The present invention discovered a strain of Trichoderma aculeatus FJ069 for the first time. The Latin taxonomic name of the strain is: Trichodema asperellum. CGMCC, address: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, deposit number: CGMCCNo.17976. [0003] The invention relates to Trichoderma aculeatus for agricultural production, in particular to a Trichoderma aculeatus FJ069 and its application. [0004] 2. Background technology [0005] At present, the prevention and control of plant diseases is still mainly based on the control of chemical agents. The use of chemical agents leads to the resistance of plant pathogens, which increases the difficulty of prevention and control. Moreover, chemical pesticides will pollute and damage the environment, leading to the destruction of the biological chain. Pesticide residues are extremely harmful to the human body. great harm. Therefore, more and more attention has been paid to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCC12R2001/885C12N1/145A01N63/30
Inventor 刘铜薛鸣陈迪刘震侯巨梅邢梦玉
Owner 贵州益百亿生物科技有限公司
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