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Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and microbial strains, applied in the field of microorganisms, can solve the problems of unmarketable and rotten fruits, weak processing ability, unsound service system, etc., and achieve good antibacterial effect

Active Publication Date: 2019-09-20
厦门元之道生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] With the popularization of the e-commerce network era, the traditional fruit wholesale sales system has been greatly challenged, and the sales of fruit that are only delivered to the wholesale market have declined year by year
Due to the fact that the fruit planting areas are basically located in remote mountainous areas, the transportation costs are extremely high, the network signal is poor, the cultural level of the fruit farmers is limited, and other factors, most fruit farmers are not suitable for the sales channels of e-commerce, and the service system is not perfect. Storage, processing Weak ability and other comprehensive factors eventually lead to many fruit growers' fruit being seriously unsalable and rotten, and the sales price is low, which has a serious impact on fruit growers and the regional economy

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The domestication screening and isolation of bacteria

[0024] Draw 1mL sample from the mixture of passion fruit pulp and honey after 21 days of constant temperature culture at 37°C, dilute 10 -1 ,10 -2 For the next step, take 0.1ml of the stock solution and the diluted solution and spread them on the plate containing the MRS solid medium. After the diluted solution is absorbed by the plate, culture it upside down at 42°C for 24 hours. Pick out the milky white single colony suspected of lactic acid bacteria and place it on the MRS solid plate. The upper line was purified and cultured for 3 times, and at the same time, a single colony was cultured in the MRS liquid medium at 37°C for 24 hours, and 1ml of the fermentation broth was mixed with 1ml of passion fruit pulp and honey mixture, and fermented at 37°C for 24 hours to generate acidity from the fermentation liquid And pH and the number of lactic acid bacteria colonies in the fermented liquid are used as in...

Embodiment 2

[0028] The identification of embodiment 2 strains

[0029] 2.1 Morphological observation of bacteria

[0030] Colony morphology observation was carried out on the bacterial cell BXM2 ( figure 1 ), observation of bacterial cell morphology by transmission electron microscope ( figure 2 ). The main morphological characteristics of BXM2 bacteria are as follows: the colony is round, milky white and opaque on the MRS medium, and the bacteria are rod-shaped, with a width of 0.6-0.8 μm and a length of 1.3-2.5 μm.

[0031] Bacteria identification system for identification and physiological and biochemical detection and analysis of bacteria

[0032] The sugar utilization and enzyme production of bacteria BXM2 were analyzed by using the API50 CHL V5.1 kit, and the results are shown in Table 1 and Table 2.

[0033] Table 1. Sugar utilization by bacteria BXM2

[0034]

[0035]

[0036]

[0037] Table 2. Analysis of enzyme production of bacteria BXM2

[0038]

[0039] No...

Embodiment 3

[0049] Example 3 Antibacterial properties of Lactobacillus plantarum BXM2

[0050] Lactobacillus plantarum BXM2 was inoculated in 100ml of MRS liquid medium, fermented and cultivated at 37°C for 24 hours to prepare the mother liquor of the strain. At the same time, the basal medium was used to inoculate and culture Escherichia coli DH5ɑ, Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Shigella sonnei, Salmonella typhimurium, and Listeria monocytogenes to prepare the bacterial mother liquid, using PDA medium Inoculate and cultivate red mold, green mold and black mold respectively, prepare fungal mother liquid, take 0.1mL and spread it on the basic medium and PDA plate medium, and use a hole puncher with a diameter of 7mm to separate the plates coated with various bacteria. Drill three wells, fill the wells with BXM2 fermented yeast solution, and incubate at a constant temperature of 37°C for 48 hours, and calculate the antibacterial effect of BXM2 on other bacteria....

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Abstract

The invention provides a lactobacillus plantarum strain, which is a Lactobacillus plantarum BXM2 and is deposited in the China General Microbiological Culture Collection Center on September 6, 2018 with the preservation number of CGMCC No. 16436. The Lactobacillus plantarum BXM2 has a better bacteriostatic effect on Proteus mirabilis, staphylococcus aureus, Shigella sonnei and Escherichia coli, can ferment and produce passion fruit fermented juice by taking sterilized or unsterilized passion fruit meat as a raw material, and provides a new fermentation bacterium source for the production and development of passion fruit fermented juice.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a strain of Lactobacillus plantarum ( Lactobacillus plantarum ) BXM2, the strain can be applied to ferment and produce passion fruit fermented juice. Background technique [0002] Passion fruit, also known as passion fruit, mainly has two kinds of purple and yellow. It contains 17 kinds of amino acids, multivitamins and carotenoids and other rich nutrients needed by the human body. Blood lipids, improve immunity and other multiple effects. Based on the above characteristics of passion fruit, passion fruit has become one of the most popular fruits. [0003] With the popularization of the e-commerce network era, the traditional fruit wholesale sales system has been greatly challenged, and the sales of fruits that are only transported to the wholesale market have been declining year by year. Because the fruit planting areas are basically located in remote mountainous are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/38C12R1/25
CPCA23L2/382C12N1/205C12R2001/25
Inventor 官雪芳林斌王琦钱蕾
Owner 厦门元之道生物科技有限公司
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