A method for reducing heavy metal residues in Ganoderma lucidum polysaccharides by membrane method

A technology for Ganoderma lucidum polysaccharides and heavy metals, which is applied to the field of reducing heavy metal residues in Ganoderma lucidum polysaccharides by membrane method, can solve the problems of poor extraction effect, high maintenance cost, complicated pretreatment, etc., so as to reduce the steps of alcohol precipitation, improve production efficiency, The effect of reducing solvent consumption

Active Publication Date: 2021-09-24
华茂(湖州)保健品有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The document of CN101367883A discloses a method for extracting Ganoderma lucidum polysaccharide from the fermentation broth. First, the Ganoderma lucidum polysaccharide fermentation broth is pretreated, and some small molecular impurities are removed by ultrafiltration, and then removed by the pretreated cation exchange resin and anion exchange resin. Impurities, the eluent can be obtained after freeze-drying, but the ion exchange resin has problems such as short service life, complicated pretreatment, high maintenance cost, not suitable for large-scale extraction, and the extraction effect is not good

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for reducing heavy metal residues in Ganoderma lucidum polysaccharides by membrane method
  • A method for reducing heavy metal residues in Ganoderma lucidum polysaccharides by membrane method
  • A method for reducing heavy metal residues in Ganoderma lucidum polysaccharides by membrane method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: Filter the Ganoderma lucidum extract through a primary filter membrane (ceramic membrane, 20nm) to obtain retentate I and permeate;

[0033] Step 2: Filter the permeate obtained in step 1 through a secondary filter membrane (nanofiltration membrane, 2nm) to obtain retentate II;

[0034] Step 3: Combine the retentate I and the retentate II, concentrate and dry to obtain the Ganoderma lucidum polysaccharide product.

Embodiment 2

[0036] Step 1: Select Ganoderma lucidum, wash it with water, slice it, dry it naturally, and then grind it for the first time;

[0037] Step 2: Mix the first pulverized ganoderma lucidum with water (the ratio of solid to liquid is 1g:5ml), treat it with microwave for 5 minutes, then treat it with ultrasonic wave for 20 minutes, place it at room temperature, and then centrifuge to obtain the filtrate and filter residue;

[0038] Step 3: adjust the pH of the obtained filtrate to 4.0, add cellulase, pectinase, and amylase, hydrolyze at 36°C for 40 minutes, and centrifuge to obtain Ganoderma lucidum extract. The amount of cellulase used is 0.2 times the mass of the filtrate. The quality dosage of gelatinase is 0.05 times of the filtrate quality, and the quality dosage of amylase is 0.3 times of the filtrate quality;

[0039] Step 4: centrifuge the obtained Ganoderma lucidum extract at room temperature at high speed (10000r / min, 8min), and collect the supernatant a;

[0040] Step ...

Embodiment 3

[0045] Step 1: Select Ganoderma lucidum, wash it with water, slice it, dry it naturally, and then grind it for the first time;

[0046] Step 2: Mix the first crushed ganoderma lucidum with water (the ratio of solid to liquid is 1g: 20ml), treat it with microwave for 7 minutes, then treat it with ultrasonic wave for 30 minutes, put it at room temperature, and centrifuge to obtain the filtrate and filter residue;

[0047] Step 3: adjust the pH of the obtained filtrate to 4.0, add cellulase, pectinase, and amylase, hydrolyze at 37°C for 30 minutes, and centrifuge to obtain Ganoderma lucidum extract. The quality dosage of gelatinase is 0.02 times of the filtrate quality, and the quality dosage of amylase is 0.8 times of the filtrate quality;

[0048]Step 4: centrifuge the obtained Ganoderma lucidum extract at room temperature at high speed (10000r / min, 8min), and collect the supernatant a;

[0049] Step 5: Adjust the pH of the supernatant a to 4 with hydrochloric acid, raise the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

A membrane method for reducing heavy metal residues in Ganoderma lucidum polysaccharides, comprising the following steps: Step 1: Filtrating the Ganoderma lucidum extract through a primary filter membrane to obtain retentate I and permeate, the primary filter membrane having a pore size of Be the ceramic membrane of 10~20nm; Step 2: filter the permeate obtained in step 1 through a secondary filter membrane to obtain retentate II, and the secondary filter membrane is a nanofiltration membrane with a pore size of 0.5~2nm; Step 3: Combine the retentate I and the retentate II, concentrate and dry to obtain the Ganoderma lucidum polysaccharide product. The invention realizes the purification of the ganoderma lucidum polysaccharide through membrane filtration, and the process can simultaneously desalinate while reducing heavy metal residues, has simple process, simple equipment structure and convenient maintenance, and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of preparation of ganoderma lucidum polysaccharides, and in particular relates to a membrane method for reducing heavy metal residues in ganoderma lucidum polysaccharides. Background technique [0002] Ganoderma lucidum is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. So far, we have isolated more than 150 compounds from Ganoderma lucidum, which can be divided into polysaccharides, triterpenes, nucleosides, furans, alkaloids, amino acids, proteins, sterols, inorganic ions, organic germanium, etc. More than a dozen types of substances, polysaccharides have the functions of anti-tumor growth, reducing the side effects of chemotherapy, calming the nerves, improving the body's immunity, strengthening the heart, lowering blood fat and blood pressure, protecting the liver, lowering...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 盛云峰谷浩谢中品
Owner 华茂(湖州)保健品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap