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Compound with anticancer activity and preparation method thereof

A technology of anti-cancer activity and compounds, applied in the field of medicine, can solve the problems that hinder the development and utilization of medicinal value of grass dragon, and achieve the effect of high purity

Active Publication Date: 2021-08-17
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few researches on the chemical constituents and pharmacological effects of Aurora at home and abroad, which hinders the development and utilization of the medicinal value of Aurora.

Method used

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  • Compound with anticancer activity and preparation method thereof
  • Compound with anticancer activity and preparation method thereof
  • Compound with anticancer activity and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for extracting a compound with anticancer activity, comprising the following steps:

[0046] 1) using solvent extraction method to extract the medicinal material Herba Aurora, and obtain the extract of Aurora Herba;

[0047]2) adding water to the Herba Dragon extract for suspension, extracting with petroleum ether to obtain a petroleum ether extract and a water isolate; adding ethyl acetate to the water isolate for extraction to obtain an ethyl acetate extract;

[0048] 3) Adsorbing the petroleum ether extract and the ethyl acetate extract with a macroporous resin respectively, and performing gradient elution with an alcohol-water mixed solution as an eluent to obtain a first fraction and a second fraction;

[0049] 4) Adsorbing the first fraction and the second fraction with a silica gel chromatographic column respectively, and performing gradient elution with dichloromethane-methanol mixed solvent as the eluent to obtain the third fraction and the fourth frac...

Embodiment 2

[0052] A method for extracting a compound with anticancer activity, comprising the following steps:

[0053] 1) Use 80% ethanol solution to impregnate the grass dragon with a solid-liquid ratio of 1:8 for 2 days, and filter to obtain the grass dragon extract;

[0054] 2) adding water to the Herba Dragon extract for suspension, extracting with petroleum ether to obtain a petroleum ether extract and a water isolate; adding ethyl acetate to the water isolate for extraction to obtain an ethyl acetate extract;

[0055] 3) Adsorb the petroleum ether extract and ethyl acetate extract with a macroporous resin respectively, use ethanol:water with a volume ratio of 1:9 to start eluting, and gradually increase the ratio of ethanol until the volume ratio of ethanol:water is 95 : 5, collect the cut that contains the first flow fraction and the second flow fraction, obtain the first flow fraction and the second flow fraction;

[0056] 4) Adsorb the first fraction and the second fraction wi...

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Abstract

The present invention provides a compound with anticancer activity, which has the following general structural formula I: the R group is CH 3 Or H, wherein, the compound is extracted from Herba Aurora. The present invention also provides a preparation method of the compound, which includes the following steps: 1) using solvent extraction method to extract the medicinal material Aurora japonicus to obtain an extract of Aurora japonicus; 2) extracting with petroleum ether and ethyl acetate to obtain the first concentrate respectively and the second concentrate; 3) adsorb the petroleum ether extract and the ethyl acetate extract with a macroporous resin respectively to obtain the first flow fraction and the second fraction; 4) adsorb the first fraction and the second fraction with a silica gel chromatographic column respectively Two fractions, obtain the third fraction and the fourth fraction; 5) adopt preparative high performance liquid chromatography to purify the third fraction and the fourth fraction respectively, and obtain the R group as CH 3 compounds and compounds whose R group is H. The compound of the present invention has antitumor activity.

Description

technical field [0001] The present invention relates to the field of medicine. More specifically, the present invention relates to a compound with anticancer activity and its preparation method. Background technique [0002] Zhuang medicine Caolong (strong name: Gvahgya croak clip) is the dried whole herb of Ludwigia hyssopifolia (G.Don) Exell of the willow vegetable family. "Guangxi Zhuang Autonomous Region Zhuang Medicine Quality Standard" records that it has heat-clearing and poisonous properties, and can pass water. , the effect of reducing swelling and pain, used to treat diseases such as Ayimi (dysentery), infectious hepatitis, and stupid floating (edema). However, at present, there are few researches on the chemical constituents and pharmacological effects of Herba Astronaus at home and abroad, which hinders the development and utilization of Herba Dragon's medicinal value. Contents of the invention [0003] An object of the present invention is to solve at least ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D313/00A61P35/00A61K31/365
CPCA61P35/00C07D313/00
Inventor 卢汝梅张进燕霍丽妮韦建华黎云清
Owner GUANGXI UNIV OF CHINESE MEDICINE
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