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Breeding method for hypoxic resistant pseudobagrus ussuriensis pedigree

A Ussuri and pedigree technology, applied in the field of artificial breeding in aquaculture, can solve the problems of poor hypoxia tolerance, restricting the healthy development of the aquaculture industry, floating heads and even pan-ponds, etc., to speed up the cultivation process, clear the source of fry, The effect of good induction of labor

Active Publication Date: 2019-09-20
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with the yellow catfish of the same family, the low-oxygen tolerance of Ussuri catfish is poor, and the change of dissolved oxygen in the culture pond is easy to cause floating heads or even flooded ponds, which also restricts the healthy development of its aquaculture industry. Therefore, It is especially necessary to carry out the artificial breeding of its hypoxia-tolerant varieties

Method used

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  • Breeding method for hypoxic resistant pseudobagrus ussuriensis pedigree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Selection of breeding parents of Ussuri bream and induction of labor

[0028] The parent of Ussuri breamus used in the present invention has been cultivated and strengthened in the previous year. Select 20 female fish, weighing between 100 and 150 grams, with a swollen, soft and elastic abdomen, and the outline of the ovaries can be seen on the abdomen, without injury. There are 6 males, with long and pointed reproductive processes and red ends, strong physique, no injuries, and a weight of 250 grams to 310 grams. After the parents are selected, they are placed in a spawning pond with a diameter of 1.7 meters and raised temporarily for 3 days, and no feed is fed during the temporary raising.

[0029] The first injection of oxytocin: preparation of LRH-A 2 Oxytocic agent, 25 micrograms of LRH-A 2 Use 1 ml of 0.75% normal saline to dissolve, and then dilute the drug with 0.75% normal saline. The injection dose for each female fish is 5 μg / kg, and the injectio...

Embodiment 2

[0031] Example 2 Artificial insemination and hatching of Ussuri catfish

[0032] Under the condition of water temperature of 20-23°C, at 14:00 on May 27, 29 hours after induced labor, the female fish's abdomen was lightly pressed, and the eggs flowed out, indicating that the effect time was reached. Fish out the brood fish, dry the male fish, cut open the abdomen and take out the testes, cut them into pieces in a mortar and grind them, then add 100 ml of Hanks liquid; squeeze the eggs of each female fish into different petri dishes, and put 20 female fish in Eleven of them were able to successfully squeeze out the eggs, and then poured the diluted male semen into the petri dish containing the eggs, and quickly stirred them with feathers to fully contact the sperm and eggs. The ratio of female to male fish was 3:1. After stirring, let it stand for 1 minute, and then arrange the fertilized eggs in 11 petri dishes evenly on the sterilized polyethylene mesh with a size of 50×60 cm...

Embodiment 3

[0034] Embodiment 3 Cultivation of Ussuri pufferfish fry

[0035]The newly hatched juveniles do not need to be fed because they rely on the yolk sac to provide all the nutrients for early development. After 4 days of hatching, the yolk was basically exhausted, and the fry opened their mouths to feed. The newly hatched Artemia nauplii were used as the opening bait, and fed 4 times a day with an interval of 6 hours. The appropriate amount of feeding was some Artemia larvae that were still not ingested 30 minutes after feeding. After feeding for 6 days, the chopped red blood worms were used as bait to continue the cultivation. During the entire fry cultivation process, suck the dirt every 8 hours and replace about 1 / 3 of the water to keep the water quality fresh. After raising to a body length of 1.5 cm in the hatching bucket, replace the large cement pond or raise it in the pond.

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Abstract

The invention discloses a breeding method for a hypoxic resistant pseudobagrus ussuriensis pedigree. The method includes the steps of parent selection, spawning induction, insemination, incubation, fry breeding, hypoxia stress, survival rate calculation and the like. By selecting parents with well-developed gonads, a good oxytocic effect can be achieved under the action of oxytocin of appropriate proportions and doses; by conducting egg squeezing, insemination, egg laying, incubation, fry breeding and the other steps on each female fish individually, a pseudobagrus ussuriensis pedigree is built; finally, by effective hypoxia stress treatment, the hypoxic resistant pedigree is bred. Through the technologies of building the pseudobagrus ussuriensis pedigree and breeding the hypoxic resistant pedigree, technology support can be provided for artificial selection of pseudobagrus ussuriensis based on several pedigrees, and meanwhile, it is of great significance for speeding up the process of breeding hypoxic resistant pseudobagrus ussuriensis new varieties.

Description

technical field [0001] The invention belongs to the technical field of artificial breeding in aquaculture, and in particular relates to a breeding method for a low-oxygen tolerant lineage of Ussuri catfish. Background technique [0002] In aquaculture, traits such as growth and stress resistance have always been important factors affecting the yield and quality of aquatic products. How to obtain new species of aquatic animals with fast growth and strong stress resistance has always been the direction of efforts of the majority of aquatic practitioners. Selective breeding has always been the most commonly used and traditional method in aquatic animal breeding, and family can provide an important carrier for the maintenance and continuation of excellent traits, so family construction is one of the important techniques in this breeding method. [0003] Ussuriensis (Pseudobagrus ussuriensis) belongs to Siluriformes, Pseudoidae, and Pseudomonas, and is distributed in various wat...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 朱传坤潘正军王辉常国亮丁怀宇聂孝燕余祥胜陈文永
Owner HUAIYIN TEACHERS COLLEGE
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