Method for identifying queshan black pig
An identification method and technology for black pigs, applied in biochemical equipment and methods, microbiological determination/testing, DNA/RNA fragments, etc., can solve the problem of similar morphological characteristics of black pig breeds
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Embodiment 1
[0049] In existing studies, the mitochondrial DNA structure of mammals is simple, stable, and follows maternal inheritance. It is often used as a genetic marker to study the origin, evolution, kinship, and population genetic structure of domestic animals. Based on this feature, the inventor amplified and compared the mitochondrial genomes of four local pigs in Henan Province (Yuxi black pig, Nanyang black pig, and Huainan pig) and found that at position 185 of the gene in the D-loop region , the Queshan black pig has a unique mutation g.185T mutation to C; in addition, at the 589th position of the D-loop region gene, the Queshan black pig has an inserted base G. Further experimental verification was carried out. The specific process is described as follows.
[0050] (1) Primer design
[0051] The inventor selected the pig D-loop region gene in NCBI (accession number: NC_000845.1), and designed a PCR primer for the upstream and downstream of the 185th base of the D-loop regio...
Embodiment 2
[0071] On the basis of Example 1, in order to verify the specificity and reliability of the mutation site, the inventor further downloaded nine other Chinese local pig breeds (Huaihua pig, Taoyuan pig, Laiwu pig, Jinhua pig, Jinhua pig) from NCBI. pigs, Meishan pigs, Wuzhishan pigs, Luchuan pigs, Min pigs, and Erhualian), three major Western commercial pig breeds (Duroc, Yorkshire, and Landrace pigs), two Southeast Asian local pig breeds (Moncai pigs, JNP pigs ) to compare the sequences one by one, the specific comparison results are as follows image 3 , Figure 4 shown. It can be seen from the figure that the D-loop regions g.185C and g.589G of Queshan black pigs are indeed breed-specific and can be used to identify Queshan black pigs.
[0072] It should be noted that, in NCBI, the specific accession numbers of the D-loop area of each pig breed: Huai pig MG250536.1, Taoyuan pig AM040653.1, Laiwu pig EF590174.1, Jinhua pig AB041477.1, Meishan pig AM040647.1, Wuzhishan pi...
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