A high-stretch, high-viscosity, self-healing double-network hydrogel for tissue adhesion and its preparation method and application

Abstract

The present invention proposes a design strategy for a novel adhesive hydrogel material, and results in a highly stretchable, self-healing, and highly adhesive biocompatible hydrogel material. This type of material is based on the design principle of a double network structure. The first layer of covalently cross-linked network is composed of polymerizable polyethylene glycol-based polymers, and the second layer of non-covalent cross-linked network is formed by entanglement of ultra-high molecular weight hydrophilic polymers. Valence crosslink network. The grid size of the first network should be larger than the effective molecular size of the macromolecules constituting the second network, and the molecular weight of the polymers constituting the second network should be greater than its critical entanglement molecular weight, so that it can form effective molecular entanglement, Endows double network hydrogels with high stretchability, self-healing properties and high adhesiveness. This new type of adhesive hydrogel can quickly and repeatedly form stable bonds with the surface of various materials, and can quickly and repeatedly self-repair after being damaged by external forces. The material can be used as a cell culture carrier, a new type of suture-free dressing, and wearable sensor applications.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a high-stretch, high-viscosity, self-healing double-network hydrogel for tissue adhesion, a preparation method and application thereof. Background technique [0002] Hydrogel is a cross-linked hydrophilic polymer, which is composed of a porous gel network, rich in water, allowing the exchange of oxygen and nutrient metabolites, and has viscoelastic characteristics similar to human ECM, so it is widely used It can be used as a platform for studying three-dimensional cellular microenvironment and cell interaction, or as a substrate for constructing engineered tissues. However, most traditional hydrogels are composed of permanent covalent bonds or long-term stable strong physical bonds, and their mechanical characteristics are mainly elastic. There are still many problems in tissue engineering applications: 1) Covalent bonds or long-term stable strong physical bonds Th...

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Problems solved by technology

However, most traditional hydrogels are composed of permanent covalent bonds or long-term stable strong physical bonds, and their mechanical characteristics are mainly elastic, so there are still many problems in tissue engineering applications: 1) Covalent bonds or long-term stable strong physical bonds The pore size of the cross-linked gel network is small, which makes the mass transfer efficiency of the cells inside the gel low and tends to apoptosis; 2) The chemically cross-linked hydrogel network area is rigid, so it can be used as a three-dimensional extracellular matrix for three-dimensional cell culture 3) Traditional hydrogels do not form adhesion to the contacting tissue / matrix surface due to the hydrophilic surface, so they are usually used to avoid Non-specific adsorption of macromolecules is used to avoid the adhesion of substances on its surface; 4) Traditional single-network hydrogels often have poor mechanical properties and cannot meet the requirements of higher mechanical properties such as high strength and high toughness

The existing commercial tissue adhesives for clinical application mainly realize the adhesion with tissues through chemical crosslinking and curing reactions, such as (CA, Dermabond, US)) (S.C. Woodward, J.B. Herrmann, J.L. Cameron, G. Brandes, E.J. Pulaski, F. Leonard, Annals of Surgery 1965, 162, 113.) and Succinimide ester (L.C. Sehl, O.M. Trollsas, D.G. Wallace, D .Toman, F.A.Delustro, J.A.Schroeder, G.H.Chu, Google Patents, 2004) and other tissue adhesive products, therefore all have the problem of cytotoxicity

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