Construction method for suckling mouse model infected by hepatitis B virus and application of method

Abstract

The invention discloses a construction method for a suckling mouse model infected by hepatitis B virus and application of the construction method. The construction method comprises the steps of extracting serum of a patient with high expression infection, eliminating spontaneous infection of HBV in an experimental mouse, injecting the serum of the patient into the mouse by a high-pressure hydrodynamic tail vein method, respectively taking blood through an intraocular canthus vein of the mouse at 7, 15 and 30 days after injection to detect the concentration of HBs Ag, HBe Ag and HBV DNA, and determining that modeling is successful when the result is positive. The method provided by the invention can be used for successfully infecting suckling mice, it detects that the successfully modeled suckling mouse can permanently have the expression of HBV within one month, meanwhile, body temperature rising and body weight changing of the suckling mouse cannot be caused, the cost is low, ethicalproblems do not exist, the suckling mouse model can be used as an animal model for researching chronic HBV infection, and the requirements of current scientific research are met.

Description

technical field [0001] The invention relates to the technical field of animal model preparation, in particular to a construction method and application of a suckling mouse model infected with hepatitis B virus. Background technique [0002] Hepatitis B is a disease that endangers the health of all human beings. It can be transmitted through blood, iatrogenic transmission, mother-to-child transmission and sexual transmission, and can be followed by liver fibrosis, cirrhosis, liver failure, and even hepatocellular carcinoma. It is estimated that about 2 billion people in the world have been infected with HBV, of which about 400 million people are chronic hepatitis B infections. Interferon α and nucleoside analogues are currently the main antiviral drugs, which can inhibit virus replication to a certain extent, reduce inflammation and necrosis of liver cells, but cannot completely remove covalently closed circular DNA (covalent-ly closed circular DNA, ccc DNA), so chronic hepa...

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Problems solved by technology

HBV transgenic mice are widely used in drug research and gene therapy to interfere with the replication of HBV, and can even be used to study the clearance of ccc DNA. However, the cost is high, the reproducibility is average, and the research on the life cycle of HBV is not yet clear; duck hepatitis B The model is mainly aimed at avian hepatotropic virus, which is quite different from human HBV in structure, and cannot reproduce the infection process of human HBV well; tree shrews are small mammals that have been found to be able to infect HBV in recent years, and the research has successfully passed the inoculation Serum infection of infected persons makes HBV replicate in vivo, but most of the tree shrews used for research are wild-type, which has disadvantages such as unclear genetic background, difficult cultivation, short and unstable expression of HBV; chimpanzees are other than humans that can be infected with HBV Primates are difficult to promote due to their high price and ethical problems

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