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Method for extracting theaflavin from black tea

A theaflavin and black tea technology, applied in the field of plant extraction, can solve the problems of complex preparation and purification process, and achieve the effect of being suitable for large-scale production and simple process

Pending Publication Date: 2019-08-30
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical oxidation method can eliminate the constraints of various influencing factors, so that the reaction process is easy to control, and the controllability is strong. However, due to the addition of chemical oxidants, the preparation and purification process is more complicated than the direct extraction method.

Method used

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  • Method for extracting theaflavin from black tea
  • Method for extracting theaflavin from black tea
  • Method for extracting theaflavin from black tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Provide five different solid-liquid ratio parameters of black tea powder and distilled water, namely 1:20, 1:30, 1:40, 1:50, 1:60.

[0034] 2. Experimental procedure: Weigh 1.000g of ground black tea powder, add 20mL, 30mL, 40mL, 50mL, 60mL of distilled water respectively. Filter under reduced pressure and cool to room temperature; use a pipette to take 5 mL of the filtrate into a test tube, add 5 mL of IBMK to the test tube, shake and separate layers; use a pipette to take 2 mL of the upper ketone layer solution into a test tube, add 0.05 mol / L AlCl 3 : the mixed solution 6ml of dehydrated alcohol volume ratio 1:2, after mixing, seal with plastic wrap, place 15min to make it react completely; Take the mixed solution of IBMK and ethanol 1:2 as reference solution, use at wavelength 525nm The visible light spectrophotometer detects the absorbance, and the test results refer to Table 3 and figure 2 . From figure 2 It can be seen that the absorbance is maximum whe...

Embodiment 2

[0038] 1. Provide six different reflow temperature parameters, namely 50°C, 60°C, 70°C, 80°C, 90°C, 100°C.

[0039] 2. Experimental procedure: Weigh 1.000g of black tea powder, add 20mL of distilled water for extraction, extract at 50°C, 60°C, 70°C, 80°C, 90°C, and 100°C for 20 minutes, vacuum filter while hot, and cool to room temperature; take 5mL of the filtrate in a test tube, add 5mL of IBMK for extraction; take 2mL of the extracted ketone layer solution in a test tube, add 0.05mol / L AlCl 3: the mixed solution 6ml of dehydrated alcohol volume ratio 1:2, make it react 15min, take the mixed solution of IBMK and ethanol 1:2 as reference solution, detect its absorbance with visible light spectrophotometer at wavelength 525nm place, test result sees Table 4 and image 3 . From image 3 It can be seen that the absorbance is maximum when the reflux temperature is 100°C.

[0040] The impact of table 4 reflux temperature on theaflavin extraction

[0041]

Embodiment 3

[0043] 1. Provide six different reflux time parameters, namely 10min, 20min, 30min, 40min, 50min, 60min.

[0044] 2. Experimental process: Weigh 1.000g of black tea powder, add 20mL of distilled water for extraction, extract at 80°C for 10min, 20min, 30min, 40min, 50min, and 60min, filter under reduced pressure while hot, and cool to room temperature; take 5mL of filtrate In the test tube, add IBMK 5mL for extraction; take 2mL of the extracted ketone layer solution in the test tube, add 0.05mol / L AlCl 3 : the mixed solution 6ml of dehydrated alcohol volume ratio 1:2, make it react 15min; With the mixed solution of IBMK and ethanol 1:2 as reference solution, detect its absorbance with visible light spectrophotometer at wavelength 525nm place, test result sees Table 5 and Figure 4 . From Figure 4 It can be seen that the absorbance is maximum when the reflux time is 30 minutes.

[0045] The impact of table 5 reflux time on theaflavin extraction

[0046]

[0047] Further...

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Abstract

The invention provides a method for extracting theaflavin from black tea. The method comprises the steps: mixing black tea powder with distilled water so as to form a mixed liquid at a solid-liquid ratio of black tea powder to distilled water at (1:20)-(1:60), heating the mixed liquid for reflux extraction at a reflux temperature of 50-100 DEG C for a reflux period of time of 10-60 min, performingvacuum suction filtration on the hot product, then performing cooling to room temperature so as to obtain a filtrate, adding isomethylbutanone or 4-methyl-2-pentanone which is adopted as an extractant for extraction so as to obtain a ketone layer containing theaflavin, and removing the ketone layer so as to obtain the theaflavin.

Description

technical field [0001] The invention relates to a plant extraction method, in particular to a method for extracting theaflavins from black tea. Background technique [0002] Theaflavins are an important factor affecting the quality of black tea, and have a variety of pharmacological and health effects, mainly including anti-oxidation, anti-cancer and anti-cancer, lowering blood fat, preventing cardiovascular diseases, antibacterial and antiviral effects, etc. It has great potential for development. With the improvement of people's living standards and the popularization of the concept of green environmental protection, people are more and more respectful of green life, and the pharmacological functions of theaflavins have also attracted more and more attention. Theaflavins are a kind of natural active ingredients, which have broad application prospects in the fields of food, medicine and health care, so the extraction and preparation of theaflavins have become a research hot...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 应少明谢丹华黄剑华
Owner NINGDE NORMAL UNIV
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