Detection method and detection kit for genotyping of human platelet alloantigen (HPA)

Abstract

The invention provides a detection method for genotyping of human platelet alloantigen (HPA). The detection method is characterized in that a target fragment is obtained by designing a specific primerand then using a single-tube multiplex PCR amplification reaction; a single base extension primer is designed and then single base extension is performed; then a target DNA sequence is accurately measured by mass spectrometry. The platelet alloantigen HPA-1 to 21 bw allele can be detected at one time. Then a mass spectrum is typed directly, and data are directly output. A whole process is closed,the time is short, and the cost is low. Through detecting the genotyping of the platelet alloantigen of a patient and a blood donor, platelets matching successfully can be found, and the problem of ineffective platelet infusion caused by immune factors is effectively avoided. A detection kit product is low in cost and more accurate, and the detection method is expected to be a routine clinical detection method to replace a serological detection method.

Description

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AI Technical Summary

Benefits of technology

In this patented technology, there are two ways we can test if someone's plasma contains certain substances called alpha hemoglobin (Hpa) or beta globin (Gp). These tests help us identify those who have an inherited abnormality from these molecules found on their own red cells. They also allow researchers to study how sick people respond differently than normal individuals during treatment with drugs like aspirin.

Problems solved by technology

This patented technical solution describes how certain types of proteins called alpha hemoglobin or beta globin help with immunity against bacterial disease like syphilis caused by gram negative bacillus parasites. These proteins were found only once during storage after being taken out of their usual environment - they could be mistaken for another virus if infected patients had symptoms similar thereto. By detecting this protein alone without performing complex procedures, there was no way to identify any new strains associated with them.

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