Method for rapidly detecting content of EGCG in tea polyphenol
A detection method and tea polyphenol technology, applied in the field of analytical chemistry, can solve the problems of high detection cost, large solvent consumption, long detection time, etc., and achieve the effects of strong elution ability, improved selectivity, and easy degassing
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[0044] (1) Setting of ultra-high performance liquid chromatography conditions:
[0045] Using Agilent ZORBAX Eclipse Plus C 18 The chromatographic column (2.1×50 mm, 1.8 μm) uses methanol-1% acetic acid as the mobile phase, the flow rate is 0.55 mL / min, the column temperature is 35 °C, the detection wavelength is 276 nm, and the injection volume is 0.50 μL. The way of elution, the specific elution conditions are shown in the table below:
[0046]
[0047] (2) Preparation of reference substance solution: Precisely weigh EGCG, EC, EGC, GCG, ECG and caffeine reference substance 1.29, 1.02, 1.43, 1.10, 1.03, 0.25 mg respectively, add absolute ethanol to dissolve and dilute to 5mL, as the reference substance solution;
[0048] (3) Preparation of the test solution: Accurately weigh 4.14mg of tea polyphenols, add absolute ethanol to dissolve and dilute to 5mL, and use it as the test solution;
[0049] (4) System suitability test:
[0050] Take the test solution of tea polyphe...
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