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Gas chromatography-mass spectrometry-based construction method for characteristic spectrum of Spina date seed oil and application thereof

A technology of sour jujube seed oil and gas chromatography, which is applied in the field of gas chromatography-mass spectrometry construction of characteristic map of sour jujube seed oil, can solve problems such as complex processing technology, and achieve the effects of accurate evaluation and simple sample pretreatment.

Active Publication Date: 2019-08-16
汉方萃取生物科技(海南)股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that the existing evaluation method for jujube kernel oil has complex processing techniques, and provides a method for constructing a characteristic map of jujube kernel oil by gas chromatography-mass spectrometry and its application

Method used

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  • Gas chromatography-mass spectrometry-based construction method for characteristic spectrum of Spina date seed oil and application thereof
  • Gas chromatography-mass spectrometry-based construction method for characteristic spectrum of Spina date seed oil and application thereof
  • Gas chromatography-mass spectrometry-based construction method for characteristic spectrum of Spina date seed oil and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Establishing the Jujube Seed Oil characteristic map, comprises the following steps:

[0024] Step (1) Collection of samples. The details are as follows: The wild jujube kernels used in the experiment were purchased from Hebei Anguo Medicinal Materials Market and Shanxi Zhendong Daodi Medicinal Materials Co., Ltd. It has been identified that Ziziphus jujube Mill.Var.spinose (Bunge) Hu ex H.F.Chou is the dry mature seed of Ziziphus jujube Mill.Var.spinose (Bunge) Hu ex H.F.Chou; Lizaoren is the dry mature seed of Ziziphus mauritiana Lam. A total of 13 batches of Jujube Seed samples were collected and preserved in the Modern Research Center of Traditional Chinese Medicine of Shanxi University.

[0025] Step (2) Suanzaoren oil extraction. The details are as follows: Take 2g of samples from each batch, place them in a Soxhlet extraction, add 90mL of petroleum ether (60-90°C), heat and reflux for 4 hours, recover the petroleum ether, and obtain Jujube Seed Oil and Jujube Se...

Embodiment 2

[0047] It is used for homogeneity evaluation and relative content determination of Jujube Seed Oil.

[0048] Characteristic spectrum can characterize the correlation between material complex composition and its quality, wherein similarity evaluation plays an important role, the jujube kernel oil similarity evaluation method that the present invention adopts is " traditional Chinese medicine chromatographic characteristic graph similarity evaluation system" recommended by National Pharmacopoeia Commission "Version 2.0 software, using the control characteristic map as a standard, calculated the similarity of 13 batches of jujube kernel oil characteristic maps, and the calculation results are shown in Table 3. It can be seen from the table that the similarities of the 13 batches of jujube seed oil samples are all between 0.996-1, which has a very high similarity, indicating that the homogeneity of the jujube kernel oil in different batches is better. Using the peak area normaliza...

Embodiment 3

[0050] Distinguish between Jujube Seed Oil and Jujube Seed Oil

[0051] The collection of samples is as follows: The jujube kernel used in the experiment was purchased from the medicinal material market in Anguo, Hebei, and it was identified as the dried and mature seed of Ziziphus mauritiana Lam., a plant of Rhamnaceae. A total of 7 jujube kernel samples were collected and preserved in the Modern Research Center of Traditional Chinese Medicine of Shanxi University.

[0052] According to the steps (2~6) in the embodiment 1, construct 7 batches of jujube kernel oil feature maps ( figure 2 ). Taking 13 batches of jujube seed oil and 7 batches of jujube kernel oil as the research objects, the original data matrix was composed of the chromatographic peak areas in the characteristic spectra of each sample, and the principal component analysis was carried out with SIMCA-P 13.0. The results are shown in Figure 5 . The cumulative variance contribution rate of the first two princi...

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Abstract

The invention discloses a gas chromatography-mass spectrometry-based construction method for a characteristic spectrum of Spina date seed oil and an application thereof, belongs to the technical fieldof detection, and aims at solving the problem that the existing methods for evaluating Spina date seed oil are complicated in treatment process. The method comprises the following steps of (1) carrying out petroleum ether Soxhlet extraction on Spina date seed powder to obtain Spina date seed oil, filtering the Spina date seed oil through a derived filter membrane to obtain a test sample; (2) measuring a chemical characteristic spectrum of the Spina date seed oil through a gas chromatography-mass spectrometry hyphenated technology; and (3) carrying out spectrum analysis to determine that the characteristic spectrum of the Spina date seed oil comprises 9 characteristic peaks. The method is concise in sample pretreatment, convenient and good in reproducibility, is capable of accurately reflecting certain important characteristic information of the Spina date seed oil, and can be used for the quality of the Spina date seed oil.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a construction method and application of a gas chromatography-mass spectrometry spectrum of jujube kernel oil, analysis and similarity evaluation of the characteristic spectrum of jujube kernel oil, and provides a new detection method for quality control of jujube kernel oil. Background technique [0002] Suanzaoren was first recorded in "Shen Nong's Materia Medica" in the late Eastern Han Dynasty, and was included in "Chinese Pharmacopoeia" in 1977. Since ancient times, it has been listed as a good medicine for calming the mind and calming the mind. It is the earliest food and Chinese medicinal material promulgated by the National Health and Family Planning Commission One of the traditional Chinese medicinal materials. Studies have shown that the main chemical components of Suanzaoren include secondary metabolites such as saponins, flavonoids, and alkaloids, and pr...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/047G01N2030/067
Inventor 闫艳申晨曦杜晨晖裴香萍张福生张丽增
Owner 汉方萃取生物科技(海南)股份有限公司
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