Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition
A technology of vibrio alginolyticus and phage, which is applied in the direction of phage, virus/phage, application, etc. It can solve the problems affecting the treatment effect and resistance mutation, achieve long-term bactericidal effect, avoid anti-phage mutation, and host specificity strong effect
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Embodiment 1
[0060] Example 1 Isolation of Vibrio alginolyticus phage ValB1 MD
[0061] Take a seawater sample, filter it through a 0.22 μm sterile filter membrane, add it to Vibrio alginolyticus VIB 283 T bacteria solution in the exponential growth phase at a volume ratio of 10%, and cultivate it on a shaking table at 28°C. Samples were taken on 1d, 3d, 5d, and 7d respectively. After the samples were filtered and sterilized by a 0.22 μm sterile filter, they were diluted with different concentration gradients with SM Buffer, and then 1ml of the diluted filtrate was mixed with 1ml of logarithmic phase Vibrio alginolyticusVIB 283 T bacteria solution Mix and incubate on a shaker at 28°C for 30 minutes, then pour into melted and pre-cooled RO semi-solid at about 48°C, mix and pour on the RO solid plate, shake evenly until the semi-solid solidifies. Place the semi-solid plate in a constant temperature incubator at 28°C overnight, and if there are plaques, it is the phage that was initially isol...
Embodiment 2
[0062] Embodiment 2 Purification of Vibrio alginolyticus phage ValB1 MD
[0063] Soak the phage plaque obtained in Example 1 in SM Buffer overnight, then filter the soaking solution through a 0.22 μm sterile filter head and then use SM Buffer to gradiently dilute, make a double-layer plate according to the method described in Example 1, and obtain a double-layer plate Evenly dispersed plaques on a plate. Repeat the above operation 3-5 times until the size and shape of the plaques on the double-layer plate obtained for the last time are consistent. Purified phage Double layer plates see figure 1 .
Embodiment 3
[0064] Example 3 Isolation of Vibrio alginolyticus phage ValB1 HC
[0065] The specific implementation method is described in Example 1.
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