Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition

A technology of vibrio alginolyticus and phage, which is applied in the direction of phage, virus/phage, application, etc. It can solve the problems affecting the treatment effect and resistance mutation, achieve long-term bactericidal effect, avoid anti-phage mutation, and host specificity strong effect

Active Publication Date: 2019-08-16
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, phage therapy also has certain limitations. For example, pathogenic bacteria can easily produce resistance mutations to a single phage infection, which affects its actual therapeutic effect.

Method used

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  • Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition
  • Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition
  • Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Example 1 Isolation of Vibrio alginolyticus phage ValB1 MD

[0061] Take a seawater sample, filter it through a 0.22 μm sterile filter membrane, add it to Vibrio alginolyticus VIB 283 T bacteria solution in the exponential growth phase at a volume ratio of 10%, and cultivate it on a shaking table at 28°C. Samples were taken on 1d, 3d, 5d, and 7d respectively. After the samples were filtered and sterilized by a 0.22 μm sterile filter, they were diluted with different concentration gradients with SM Buffer, and then 1ml of the diluted filtrate was mixed with 1ml of logarithmic phase Vibrio alginolyticusVIB 283 T bacteria solution Mix and incubate on a shaker at 28°C for 30 minutes, then pour into melted and pre-cooled RO semi-solid at about 48°C, mix and pour on the RO solid plate, shake evenly until the semi-solid solidifies. Place the semi-solid plate in a constant temperature incubator at 28°C overnight, and if there are plaques, it is the phage that was initially isol...

Embodiment 2

[0062] Embodiment 2 Purification of Vibrio alginolyticus phage ValB1 MD

[0063] Soak the phage plaque obtained in Example 1 in SM Buffer overnight, then filter the soaking solution through a 0.22 μm sterile filter head and then use SM Buffer to gradiently dilute, make a double-layer plate according to the method described in Example 1, and obtain a double-layer plate Evenly dispersed plaques on a plate. Repeat the above operation 3-5 times until the size and shape of the plaques on the double-layer plate obtained for the last time are consistent. Purified phage Double layer plates see figure 1 .

Embodiment 3

[0064] Example 3 Isolation of Vibrio alginolyticus phage ValB1 HC

[0065] The specific implementation method is described in Example 1.

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Abstract

The invention provides a vibrio alginolyticus bacteriophage, a bacteriophage composition and an application of the vibrio alginolyticus bacteriophage and the bacteriophage composition and belongs to the technical of microbial control. Two new strains of the vibrio alginolyticus bacteriophage are obtained by screening, and the new strains have strong host specificity and strong cracking and killingeffect on vibrio alginolyticus; meanwhile, the mixed use of the new strains can effectively prevent the vibrio alginolyticus from producing resistant mutation, so as to achieve the ideal pathogen removal effect, and the important application value in the prevention and control of vibrio alginolyticus infectious diseases in marine aquaculture animals is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to Vibrio alginolyticus phages and phage compositions and applications thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Vibrio alginolyticus is a common opportunistic pathogenic bacteria in aquaculture fishery. It can infect seawater fish and freshwater fish. It manifests as extensive skin damage and bacteremia in fish. In severe cases, it can develop muscle and multiple organ edema and further lead to the death of fish. my country is a large aquaculture country, and the infection of Vibrio alginolyticus causes huge economic losses to the aquacul...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K35/76A61P31/04A01N63/00A01P1/00C12R1/92
CPCA01N63/00A61K35/76A61P31/04C12N7/00C12N2795/10121C12N2795/10131C12N2795/10132
Inventor 张永雨李成成
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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