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Biological weed control compound and preparation method thereof

A technology of herbicides and compounding agents, applied in the direction of herbicides and algicides, botanical equipment and methods, biocides, etc., can solve the problems of unsatisfactory manual removal and mechanical control, and difficult chemical control, etc., to reduce biological Control cost, good weed control effect, strong host specific effect

Active Publication Date: 2013-06-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods have certain effects, due to the strong reproductive ability and stress resistance of the grass, manual removal and mechanical control are not ideal, and chemical control is also relatively difficult.
[0007] In terms of biological control research on crabgrass weeds, the inventor found that conidia and hyphae of a fungal strain of Curvularia can be used for weed control (patent No. CN01134002. Further research is needed on grass

Method used

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  • Biological weed control compound and preparation method thereof
  • Biological weed control compound and preparation method thereof
  • Biological weed control compound and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Conidia culture: Cultivate QZ-2000 in a medium for 7 days, which contains potato, glucose and agar; take small colonies and transfer them to wheat bran-sawdust or wheat bran solid medium Cultivate for 7 days, cut the bacteria into small pieces and spread them in a flat container with a thickness of 1cm. Place them under a black light for continuous irradiation for 48 hours, air-dry and crush, and then sieved to obtain spore powder; or inoculate small colonies into corn Shake culture in the powder-soy powder-sucrose liquid medium for 6 days. After filtering out the residual medium, rinse with sterile water to obtain mycelium. After crushing, the suspension of mycelium fragments is obtained, which is illuminated in a petri dish. 36h, get spores;

[0041] (2) Prepare chemical herbicide mother liquor: prepare the mother liquor of the chemical herbicide nicosulfuron preparation diluted one hundred times, take 1ml nicosulfuron preparation and add it to 100ml water, and mix w...

Embodiment 2

[0044] (1) Conidia culture: Cultivate QZ-2000 in a medium for 7 days, which contains potato, glucose and agar; take small colonies and transfer them to wheat bran-sawdust or wheat bran solid medium Cultivate for 7 days, cut the bacteria into small pieces and spread them in a flat container with a thickness of 1cm. Place them under a black light for continuous irradiation for 48 hours, air-dry and crush, and then sieved to obtain spore powder; or inoculate small colonies into corn Shake culture in the powder-soy powder-sucrose liquid medium for 6 days. After filtering out the residual medium, rinse with sterile water to obtain mycelium. After crushing, the suspension of mycelium fragments is obtained, which is illuminated in a petri dish. 36h, get spores;

[0045] (2) Prepare the mother liquor of chemical herbicide: prepare the mother liquor of the chemical herbicide oxyfluorfen preparation diluted one hundred times, take 1ml of oxyfluorfen preparation into 100ml water, and mix we...

Embodiment 3

[0048] (1) Conidia culture: Cultivate QZ-2000 in a medium for 7 days, which contains potato, glucose and agar; take small colonies and transfer them to wheat bran-sawdust or wheat bran solid medium Cultivate for 7 days, cut the bacteria into small pieces and spread them in a flat container with a thickness of 1cm. Place them under a black light for continuous irradiation for 48 hours, air-dry and crush, and then sieved to obtain spore powder; or inoculate small colonies into corn Shake culture in the powder-soy powder-sucrose liquid medium for 6 days. After filtering out the residual medium, rinse with sterile water to obtain mycelium. After crushing, the suspension of mycelium fragments is obtained, which is illuminated in a petri dish. 36h, get spores;

[0049] (2) Prepare the mother liquor of chemical herbicides: prepare the mother liquor of the chemical herbicide butachlor and oxadiazon preparation diluted one hundred times, take 1ml of oxyfluorfen preparation and add it to 1...

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Abstract

The invention discloses a weed control compound and a preparation method thereof. The weed control compound is mainly composed of conidium of Curvularia fungus strain QZ-2000 with preservation number CGMCC NO.0640 and a chemical herbicide, wherein the concentration of the conidium is 1-10g / L, and the concentration of the chemical herbicide is 1 / 20-1 / 3 of a conventional dosage. The invention adopts that the conidium is compounded with the chemical herbicide; on one hand, conidium dosage is lowered, biological prevention and cure cost is lowered, and the chemical herbicide performs the synergy when compound compared with the situation that only spore is adopted; on the other hand, lowers pollution of chemical herbicide is lowered.

Description

Technical field [0001] The invention relates to a herbicide compounding agent, in particular to a compounding agent composed of spores of a strain of Curvularia and a chemical herbicide. Background technique [0002] Weeds have always been considered as one of the biological disasters that seriously affect agriculture and the environment. For more than a century, chemical herbicides have been effectively used to control weeds in farmland. However, due to the large-scale application of chemical herbicides, many serious problems such as the formation of resistant weeds, the decline of soil and water quality, herbicide residues, water pollution, and the emergence of resistant (tolerant) weeds are becoming more and more serious. Moreover, the extensive development and use of chemical herbicides has not completely solved the problem of weed damage. With the increasing awareness of global environmental protection, the research on biological herbicides with high herbicidal activity an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N47/36A01N33/22A01N43/824A01N37/26A01P13/00
Inventor 强胜陆艳娜朱云枝高同军徐春凤陈世国
Owner NANJING AGRICULTURAL UNIVERSITY
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