Plate fruiting method for morchella

A technology of Morchella and flat plate, applied in chemical instruments and methods, botanical equipment and methods, mushroom cultivation and other directions, can solve the problems of high labor intensity, poor yield stability, and no fruiting on flat plates, and achieves increased sclerotia. Number, simple operation, the effect of speeding up the formation time of primordium

Inactive Publication Date: 2019-08-16
四川天仁百益农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This characteristic of hickory chick cultivars makes the cultivation process labor-intensive and yield stability poor
[0004] At present, most of the morel mushrooms are cultivated in the field to produce mushrooms, and there is no method for flat plate fruiting

Method used

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  • Plate fruiting method for morchella
  • Plate fruiting method for morchella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Prepare 1 L of PDA medium, sterilize with high temperature and moist heat, add 50 mL of soil filtrate (1 g / mL) filtered by a cell strainer, add 25 mL of medium improver, and mix to obtain an improved PDA medium. Pour 20mL of the modified PDA medium into each plate, a total of 20 plates, and place them on the ultra-clean table to solidify naturally, as the test group;

[0048] (2) PDA medium is set as the control group: each plate is poured into 20mL PDA medium, totally 20 plates; wherein the plates used by the control group and the experimental group are exactly the same;

[0049] (3) picking an equivalent amount of Morchella importuna mycelium and inoculating them on PDA medium and improved PDA medium respectively;

[0050] (4) The experimental group and the control group were placed in a fungal incubator for dark culture, and the culture temperature was 18°C;

[0051] (5) Observe the growth of hyphae and the appearance time of sclerotia.

[0052] The improved me...

Embodiment 2

[0055] (1) Prepare 1 L of PDA medium, sterilize with high temperature and moist heat, add 50 mL of soil filtrate (1 g / mL) filtered by a cell strainer, add 25 mL of medium improver, and mix to obtain an improved PDA medium. Each petri dish is poured into the improved PDA culture medium 20mL after processing, altogether 40 petri dishes are placed on the ultra-clean platform to solidify naturally; each petri dish is inoculated with the mycelium of the same amount of Morchella spp. as in Example 1;

[0056] (2) Place 40 plates in a fungal incubator for dark cultivation;

[0057] Among them, 20 plates were set as the control group, and the culture temperature was set to 18°C;

[0058] The other 20 plates are set as the experimental group, and the set temperature is: 0~12h after inoculation, keep the temperature at 24°C; 12~36h, keep the temperature at 16°C; 36~72h, keep the temperature at 18°C; after 72h, keep the temperature at 16°C ;

[0059] (3) Observe the growth of mycelia, ...

Embodiment 3

[0063] (1) Prepare 1L of PDA medium, sterilize with high temperature and moist heat, add 50mL of soil filtrate (1g / mL) filtered by a cell filter, add 25mL of medium improver, pour 20mL of the improved PDA medium into each plate, and add 40 petri dishes are placed on the ultra-clean bench to solidify naturally; each petri dish is inoculated with the mycelia of the morel stalks of the same amount as in Example 1;

[0064] (2) Placed in a fungal incubator for cultivation, the cultivation temperature is 18°C;

[0065] Wherein, 20 plates were set as a control group, and the control group was cultured in the dark;

[0066] The other 20 plates were set as the experimental group, and the experimental group was also cultured in the dark, but it needed to be supplemented with light at key nodes. 12 hours after inoculation, 200 lux white light treatment for 1 hour; 36 hours after inoculation, 200 lux white light treatment for 1 hour; 72 hours after inoculation , 100 Lux illuminance whit...

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Abstract

The invention relates to a plate fruiting method for morchella and belongs to the field of fungus cultivation. The plate fruiting method for morchella comprises steps as follows: a, soil with high organic matter content, morchella stipe soil and water are mixed uniformly, the mixture is centrifuged, a liquid supernatant is taken, filtration is performed, and soil filtrate is obtained; b, a culturemedium modifier and the soil filtrate obtained in the step a are added to a PDA (potato dextrose agar) culture medium and mixed uniformly, and an efficient morchella culture medium is obtained, and the culture medium is cooled and solidified for later use; c, the efficient morchella culture medium is inoculated with morchella mycelia, culturing in a dark place is performed at the culturing temperature of 14-24 DEG C, and morchella fruiting bodies are obtained. By means of the method, the growth speed of the mycelia can be increased, sclerotium formation is accelerated, the number of sclerotiais increased, primordium formation is accelerated, and the number of primordia is increased.

Description

technical field [0001] The invention relates to a method for fruiting morel mushrooms on a plate, and belongs to the field of fungus cultivation. Background technique [0002] Morchella, which belongs to Ascomycota, Discomycetes, Pezizales, and Morchellaceae, is named for its cap with irregular depressions and folds, and its shape resembles morel. Morchella is rich in nutrition and delicious. As a world-renowned delicious edible and medicinal fungus, it is one of the most expensive wild mushrooms at present. [0003] In recent years, the artificial cultivation of hickory chick in China has developed rapidly, and the cultivation methods are various, and the cultivation scale is expanding year by year. The cultivation situation in recent years shows that Morchella is a kind of low-temperature and high-humidity bacteria. Most of the existing cultivars are black morels with caps from brown to black. They are saprophytic trophic types. The content requirements are relatively hi...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20B01D61/00
CPCA01G18/00A01G18/20B01D61/00
Inventor 彭天祥时小东庄国庆
Owner 四川天仁百益农业开发有限公司
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