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Composite microbial agent preparation device and method for river pollution control

A compound microbial agent and preparation device technology, which is applied in the field of river pollution control, can solve the problems of long treatment time, complex components of polluted water, large dosage, etc., achieve efficient treatment and improve the effect of physiological activity function

Pending Publication Date: 2019-08-09
HUNAN AIRBLUER ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to people's misunderstanding and one-sided understanding, the bacteria injection method has not received due attention in the treatment of water environmental pollution. However, whether it is from the functional requirements or the principle analysis, the bacteria injection restoration technology is the most effective way to deal with eutrophic water bodies. One of the appropriate and effective means, it can be the most effective and rapid technical measure to deal with emergencies of water quality deterioration
[0004] Due to the complex composition of polluted water, it is impossible to completely solve the problem with a single microbial agent, and the treatment time is long and the amount is large

Method used

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  • Composite microbial agent preparation device and method for river pollution control
  • Composite microbial agent preparation device and method for river pollution control

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Experimental program
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Effect test

Embodiment 1

[0054] Such as figure 1 Shown, a kind of preparation device and method of composite microbial bacterial agent for river pollution control, its preparation device comprises slope culture device 1, shake flask culture device 2, primary seed small tank 3, primary batching tank 4, primary Small seed tank pH regulator 5; secondary seed tank 6, secondary batching tank 7, secondary seed tank pH regulator 8; fermentation tank 9, fermentation tank feed tank 10, fermentation tank pH regulator 11, fermentation tank ingredients Tank 12; liquid packaging device 13, inclined tube sedimentation tank 14, diaphragm pump 15, plate and frame filter press 16, double drum filter press 16, double drum dryer 17, pulverizer 18, solid packaging device 19; sterile filtration Back steam main pipe 20, air main pipe 21 after sterilization filtration.

[0055] The slope culture device 1 is a test tube slope, and there are 6 sets of slopes in total.

[0056] The shaking flask culture device 2 is a triangu...

Embodiment 2

[0077] A method for preparing a compound microbial agent preparation device for river pollution, comprising the steps of:

[0078] 1. Preparation method

[0079] a) Strains

[0080] Pseudomonas pseudoalcaligenes, Nitrobacter, Nitrosococcus, Bacillus subtilis, Clostridium, Lactobacillus curvatus.

[0081] b) Slant 1 strain activation

[0082] Slant 1 seed medium composition: beef extract 1%, peptone 1%, Nacl 0.5%, glucose 0.5%, agar 2% (above is the volume fraction), pH7.2~7.5. Sterilized at 0.1Mpa.30min, and kept at 30°C for 24h.

[0083] Streak inoculation of the preserved strains of the above 6 strains on the slant seed medium of the respective strain test tubes by streak inoculation, and culture at 30~32°C for 18~24h to activate the above 6 strains.

[0084] c) Shake flask 2 culture

[0085] Shake Flask 2 Seed Medium Composition: Glucose 3.0%, (NH 4 ) 2 SO 4 0.5%, K 2 HPO 4 0.2%, corn syrup 2~3%, peanut cake hydrolyzate 2~3%, MgSO 4 ·7H 2 O0.06~0.08%, urea 0.2% ...

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Abstract

The invention discloses a composite microbial agent preparation device and method for river pollution control. The preparation device comprises a slant culture device (1), a shake flask culture device(2), a first-stage seed small tank (3), a second-stage seed tank (6), a fermentation tank (9) and the like which are sequentially connected; the preparation device is designed for culturing and preparing a composite microbial agent; according to the method, by using the devices, large quantities of six different dominant strains are inoculated and cultured through a specific fermentation process,so that cultures of six different types jointly survive to form a powerful functional group, various antioxidant substances are generated, the mutual growth is promoted, the physiological activity function of the whole composite flora is improved accordingly, and the prepared composite microbial agent can efficiently treat organic nitrogen and phosphorus in polluted rivers.

Description

technical field [0001] The invention belongs to the field of river pollution control, and further refers to a compound microbial agent preparation device and method for river pollution control. Background technique [0002] In recent years, with the increasing development and utilization of environmental resources, on the one hand, pollutants containing nitrogen and phosphorus nutrients have been continuously discharged into lakes, reservoirs and rivers, increasing the nutrient load of water bodies. On the other hand, in order to increase the yield of crops, the amount of chemical fertilizers and livestock manure applied to farmland has increased year by year, and the nutrients entering the water body have continuously increased through the erosion and infiltration of rainwater. Due to the influence of these human factors, the problems of water pollution and eutrophication are becoming more and more serious. For the restoration of open water systems such as lakes and rivers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/36C12M1/34C12M1/24C12M1/04C12M1/02C12N1/20C12R1/01C12R1/125C12R1/145C12R1/225
CPCC12M23/08C12M23/58C12M27/02C12M29/06C12M41/32C12M41/26C12M41/12C12M47/12C12M47/14C12N1/20
Inventor 游建军曾睿胡志鑫张振兴杨攀唐传祥
Owner HUNAN AIRBLUER ENVIRONMENTAL PROTECTION TECH
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