Application of a compound probiotic agent for preparing products for treating ulcerative colitis
A technology for ulcerative colitis and compound probiotics, which is used in the preparation of products for the treatment of ulcerative colitis and the field of compound probiotics, can solve the problem of low remission rate of ulcerative colitis, low selectivity of flora, and damage to the intestinal tract of patients. It can improve the immune system of the human body, improve the balance system, and promote the repair of various functions of the body.
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[0079] The preparation of embodiment 1 compound probiotic bacterial agent
[0080] The following takes Lactobacillus casei Zhang (Lactobacillus casei Zhang) as an example to illustrate the preparation of bacterial powder.
[0081] (1) Activation and cultivation of bacteria
[0082] The cryopreserved Lactobacillus casei Zhang (Lactobacillus casei Zhang) was inoculated in MRS liquid medium, cultured at 37°C for 20 hours, and then subcultured three times to obtain activated strains;
[0083] Among them, the MRS liquid medium was prepared according to the following proportions: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80, 0.2g Add magnesium sulfate and 0.05g manganese sulfate to 1000mL distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15min.
[0084] (2) High-density fermentation
[0085] The activated Lactobacillus casei Zhang was inoculated into a...
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