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Preparation method of amino and polypeptide modified AIE polymer nanoparticles

A nanoparticle and polypeptide modification technology, applied in chemical instruments and methods, luminescent materials, etc., can solve problems such as limited applications

Active Publication Date: 2019-08-06
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, the surface of AIE polymer nanoparticles prepared by miniemulsion polymerization has not been modified with specific peptides, which limits its application in the field of selective cell imaging to a certain extent.

Method used

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  • Preparation method of amino and polypeptide modified AIE polymer nanoparticles
  • Preparation method of amino and polypeptide modified AIE polymer nanoparticles
  • Preparation method of amino and polypeptide modified AIE polymer nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Weigh 0.2g of O-50 emulsifier and 0.1g of 2-aminoethyl methacrylate hydrochloride, and dissolve them in 12.5g of deionized water to obtain an aqueous phase solution.

[0084] Weigh 0.01g of AIE-6 molecule and dissolve it in a mixed solution of 0.06g of n-hexadecane and 0.9g of methyl methacrylate to obtain an oil phase solution; add the water phase solution to the oil phase solution and pre-emulsify by stirring Finally, a coarse emulsion was obtained; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonication with a power of 400W for 9 minutes; Amidine hydrochloride, the temperature was raised to 65° C., and reacted for 6 hours under the protection of nitrogen to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h on the first day, ...

Embodiment 2

[0101] Weigh 0.5 g of Tween 20 and 0.15 g of 2-aminoethyl methacrylate hydrochloride, dissolve them in 15 g of deionized water, and obtain an aqueous phase solution.

[0102] Weigh 0.0075g of AIE-12 molecule and dissolve it in the mixed solution of 0.08g of n-hexadecane and 1.35g of styrene to obtain an oil phase solution; add the water phase solution to the oil phase solution and pre-emulsify it with stirring to obtain Coarse emulsion; place the container containing the coarse emulsion in an ice-water bath, and use an ultrasonic wave with a power of 200W for 20 minutes to obtain a stable monomer fine emulsion; pass nitrogen to remove oxygen, add 0.015g of azobisisobutylamidine hydrochloride salt, the temperature was raised to 60° C., and reacted for 24 hours under the protection of nitrogen to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1The dialysis bag was dialyzed for three days, and the water change frequency...

Embodiment 3

[0108] Weigh 0.3g of MOA-9 and 0.05g of 2-aminoethyl methacrylate hydrochloride, dissolve them in 15g of deionized water, and obtain an aqueous phase solution.

[0109] Weigh 0.05g of AIE-15 molecules and dissolve in a mixed solution of 0.08g n-hexadecane, 0.45g styrene and 0.5g methyl methacrylate to obtain an oil phase solution; add the water phase solution to the oil phase solution , after stirring and pre-emulsifying, a coarse emulsion was obtained; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonication with a power of 600W for 5 minutes; nitrogen and deoxygenation, adding 0.05g Azobisisobutylamidine hydrochloride, the temperature was raised to 65° C., and reacted for 3 hours under the protection of nitrogen to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change...

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Abstract

The invention relates to a preparation method of amino and polypeptide modified AIE polymer nanoparticles, the preparation method comprises the following steps of: 1) dissolving an emulsifier and an amino functional monomer in water to obtain an aqueous phase solution; 2) dissolving AIE molecules and an Austrian ripening effect inhibitor in a hydrophobic monomer to obtain an oil phase solution; 3)adding the aqueous phase solution into the oil phase solution, stirring and pre-emulsifying to obtain crude emulsion, and performing ultrasonic treatment to obtain monomer miniemulsion; introducing nitrogen to remove oxygen, adding a water-soluble initiator to react to prepare amino modified AIE polymer nanoparticle emulsion; 4) dissolving omega-maleimide alkyl acid and a carbodiimide condensingagent in an acidic pH buffer solution for activation to obtain an activated intermediate solution; 5) adding the activated intermediate solution into the emulsion prepared in the step 3) to react to prepare maleimide modified AIE polymer nanoparticle emulsion; and 6) adding an aqueous solution of a polypeptide containing cysteine sequence units at the end to the emulsion prepared in the step 5) for reaction to obtain the amino and polypeptide modified AIE polymer nanoparticles.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing surface-modified aggregation-induced emission (AIE) polymer nanoparticle. [0003] (2) Background technology [0004] Fluorescent nanoparticles are an important class of functional nanomaterials, which have the characteristics of good water dispersibility, high photostability, low cytotoxicity, and surface modification. They are important in many high-end fields such as cell imaging, disease diagnosis, and biosensing. application value. However, nanoparticles with traditional aggregation-induced quenching (ACQ) fluorescent molecules as functional components often have defects such as low fluorescence quantum yield and narrow fluorescence intensity control range, which limits their application value to a certain extent. In 2001, Academician Tang Benzhong's research group first reported a new type of fluorescent molecule with AIE effect, which emits strong fluorescence due to the restricted rotat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/14C08F220/34C08F212/08C08F220/18C08F220/60C08F2/44C08F2/30C08K5/315C08K5/5399C08K5/55C08K5/3415C08K5/3437C08K5/46C08F8/30C08F8/34C09K11/02C09K11/06
CPCC08F2/30C08F2/44C08F8/34C08F212/08C08F220/14C08K5/315C08K5/3415C08K5/3437C08K5/46C08K5/5399C08K5/55C09K11/02C09K11/06C08F220/34C08F8/30C08F220/1804C08F220/60
Inventor 曹志海胡亚新齐琪王思彦温礼馨裘乘锦梁小琴秘一芳
Owner ZHEJIANG SCI-TECH UNIV
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